A5028178559- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Yersinia bacterium, plague, ectoparasites research
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Pneumonia and Respiratory Infections
- Algal biology and biofuel production
- Polysaccharides and Plant Cell Walls
- Oral microbiology and periodontitis research
- Bacterial Infections and Vaccines
- Bacillus and Francisella bacterial research
- Agronomic Practices and Intercropping Systems
- Coastal wetland ecosystem dynamics
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Microbial Community Ecology and Physiology
- Infant Nutrition and Health
- Antimicrobial Peptides and Activities
- Nematode management and characterization studies
- Streptococcal Infections and Treatments
- Phytoplasmas and Hemiptera pathogens
University of Georgia
2013-2025
SP Technology (South Korea)
2020
University of Silesia in Katowice
2006-2013
Universidade Federal do Paraná
2010
Gram-negative bacteria including Escherichia coli, Citrobacter rodentium, Salmonella typhimurium, and Shigella flexneri are sensed in an ill-defined manner by intracellular inflammasome complex that activates caspase-11. We show macrophages loaded with synthetic lipid A, E. coli lipopolysaccharide (LPS), or S. typhimurium LPS activate caspase-11 independently of the receptor Toll-like 4 (TLR4). Consistent A triggering noncanonical inflammasome, containing a divergent structure antagonized...
The high mortality of invasive fungal infections, and the limited number inefficacy antifungals necessitate development new agents with novel mechanisms targets. cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive. Here we report architecture walls pathogenic fungus Aspergillus fumigatus. Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization glycosyl linkage analysis, reveals that chitin...
Rhizobial surface polysaccharides are required for nodule formation on the roots of at least some legumes but mechanisms by which they act yet to be determined. As a first step investigate function exopolysaccharide (EPS) in determinate nodules, we isolated Mesorhizobium loti mutants affected various steps EPS biosynthesis and characterized their symbiotic phenotypes two Lotus spp. The wild-type M. R7A produced both high molecular weight lower (LMW) polysaccharide fractions whereas most...
Lipopolysaccharides (LPS) are among the known pathogen-associated molecular patterns (PAMPs). LPSs potent elicitors of PAMP-triggered immunity (PTI), and bacteria have evolved intricate mechanisms to dampen PTI. Here we demonstrate that Xylella fastidiosa (Xf), a hemibiotrophic plant pathogenic bacterium, possesses long chain O-antigen enables it delay initial recognition, thereby allowing effectively skirt elicitation innate establish itself in host. Lack modifies perception Xf hallmarks...
Bacteria can repurpose their own bacteriophage viruses (phage) to kill competing bacteria. Phage-derived elements are frequently strain specific in killing activity, although there is limited evidence that this specificity drives bacterial population dynamics. Here, we identified intact phage and derived a metapopulation of wild plant-associated Pseudomonas genomes. We discovered the most abundant viral cluster encodes remnant resembling tail called tailocin, which bacteria have co-opted...
Microalgae are a renewable and promising biomass for large-scale biofuel, food nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition organization as it can limit access to high-value molecules. Here we provide an atomic-level model non-crystalline water-insoluble glycoprotein-rich Chlamydomonas reinhardtii. Using in situ solid-state sensitivity-enhanced nuclear magnetic resonance, reveal unprecedented details protein carbohydrate...
ABSTRACT Francisella tularensis is a category A agent of biowarfare/biodefense. Little known about the regulation virulence gene expression in spp. Comparatively few regulatory factors exist , including those belonging to two-component systems (TCS). However, orphan members typical TCS can be identified. To determine if affect expression, encoding product with high similarity Salmonella PmrA response regulator (FTT1557c/FNU0663.2) was deleted novicida (a model organism for F. ). The pmrA...
Rhizobium leguminosarum is a soil bacterium with the ability to form nitrogen-fixing nodules on roots of leguminous plants. Soil-dwelling, free-living R. often encounters desiccation stress, which impacts its survival within soil. The mechanisms by bacteria resist effects stress have been described. However, role cell envelope in tolerance rhizobia relatively uncharacterized. Using transposon mutagenesis approach, mutant bv. viciae was isolated that highly sensitive desiccation. mutation...
Microalgae are photosynthetic organisms widely distributed in nature and serve as a sustainable source of bioproducts. Their carbohydrate components also promising candidates for bioenergy production bioremediation, but the structural characterization these heterogeneous polymers cells remains formidable problem. Here we present applicable protocol identifying quantifying glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl...
Significance Plant cell surface receptors perceive carbohydrate signaling molecules and hereby establish communication with surrounding microbes. Genetic studies have identified two different classes of lysin motif receptor kinases as gatekeepers that together trigger the symbiotic pathway in plants; however, no structural or functional data perception mechanisms switching these from resting state into activation is known. In this study, we use biology, biochemical, genetic approaches to...
A rapid and simple method was proposed for differentiation classification of eleven bacterial endotoxins based on surface enhanced Raman scattering (SERS) advanced machine learning algorithms.
Abstract Members of microbial communities can substantially overlap in substrate use. However, what enables functionally redundant microorganisms to coassemble or even stably coexist remains poorly understood. Here, we show that during unstable successional dynamics on complex, natural organic matter, bacteria by partitioning low-concentration substrates though they compete for one simple, dominant substrate. We allowed ocean self-assemble leachates the brown seaweed Fucus vesiculosus and...
The lipopolysaccharide (LPS) of the Gram-negative legume symbiont Rhizobium leguminosarum biovar viciae 3,841 contains several unique modifications, including addition a 27-hydroxyoctacosanoic acid (27OHC28 : 0), also termed very long chain fatty (VLCFA), attached at 2' position lipid A. A transposon mutant that lacks expression two putative 3-oxo-acyl [acyl-carrier protein] synthase II genes, fabF1 and fabF2, from VLCFA biosynthetic cluster, was isolated characterized. MS indicated lacked...
Receptors that distinguish the multitude of microbes surrounding plants in environment enable dynamic responses to biotic and abiotic conditions encountered. In this study, we identify characterise a glycan receptor kinase, EPR3a, closely related exopolysaccharide EPR3. Epr3a is up-regulated roots colonised by arbuscular mycorrhizal (AM) fungi able bind glucans with branching pattern characteristic surface-exposed fungal glucans. Expression studies cellular resolution show localised...
Abstract Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition responses to microbial associated molecular patterns in mammals plants. In legumes, EPS are monitored can either block or promote symbiosis with rhizobia depending on their composition. To establish a deeper understanding receptors involved recognition, we determined the structure Lotus japonicus ( ) exopolysaccharide receptor 3 (EPR3)...
Apolipoprotein A-I (apoA-I), the main protein component of high density lipoprotein (HDL), is well recognized for its antiatherogenic, antioxidant, and antiinflammatory properties. Here, we report a novel role apoA-I as host defense molecule that contributes to complement-mediated killing an important gastrointestinal pathogen, Gram-negative bacterium Yersinia enterocolitica. We specifically show C-terminal domain effector site providing bactericidal activity. Although presence...
The symbiosis between
ABSTRACT Paraburkholderia phymatum, a β-proteobacterium, forms nitrogen-fixing symbiosis with many species of the large legume genus Mimosa as well common bean ( Phaseolus vulgaris L.). are considered to have evolved nodulation independently from well-studied α-proteobacteria symbionts legumes. However, detailed mechanisms important for β-rhizobia-legume not yet been determined. In this manuscript, we sequenced genome P. phymatum MP20, strain isolated pudica nodules, and utilized transposon...
ABSTRACT Campylobacter jejuni is a common foodborne pathogen worldwide that associated with high rates of morbidity and mortality among infants in low- to middle-income countries (LMICs). Human milk provides an important source nutrients contains antimicrobial components for protection against infection. However, recent studies, including our own, have found significantly higher levels diarrheal stool samples collected from breastfed compared non-breastfed LMICs. We hypothesized C. has...
The lipooligosaccharide (LOS) of Neisseria meningitidis can be decorated with phosphoethanolamine (PEA) at the 4' position lipid A and O-3 O-6 positions inner core heptose II residue. biological role PEA modification in N. remains unclear. During course our studies to elucidate pathogenicity ST-2032 (invasive) meningococcal clonal group, disruption lptA, gene that encodes transferase for A, led a approximately 10-fold decrease adhesion four kinds human endothelial epithelial cell lines an...
Yersinia pestis, the causative agent of plague, autoaggregates within a few minutes cessation shaking when grown at 28 degrees C. To identify autoaggregation factor Y. we performed mariner-based transposon mutagenesis. Autoaggregation-defective mutants from three different pools were identified, each with insertion position gene encoding phosphoglucomutase (pgmA; y1258). Targeted deletion pgmA in pestis KIM5 also resulted loss autoaggregation. Given previously defined role for antimicrobial...
Rhizobial lipopolysaccharide (LPS) is required to establish an effective symbiosis with its host plant. An exo5 mutant of Rhizobium leguminosarum RBL5523, strain RBL5808, defective in UDP-glucose (Glc) dehydrogenase that converts UDP-Glc UDP-glucuronic acid (GlcA). This unable synthesize either UDP-GlcA or UDP-galacturonic (GalA) and extracellular capsular polysaccharides, lacks GalA LPS (Laus MC, Logman TJ, van Brussel AAN, Carlson RW, Azadi P, Gao MY, Kijne JW. 2004. Involvement production...