A5052627096- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Listeria monocytogenes in Food Safety
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Food Safety and Hygiene
- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Meat and Animal Product Quality
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Antifungal resistance and susceptibility
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- Antibiotic Use and Resistance
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- Metal Extraction and Bioleaching
- Microbial Metabolism and Applications
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- RNA modifications and cancer
Technical University of Denmark
2008-2024
University of Copenhagen
2009-2019
Food Research Institute
2018
Antimicrobial resistance (AMR) is a serious threat to global public health, but obtaining representative data on AMR for healthy human populations difficult. Here, we use metagenomic analysis of untreated sewage characterize the bacterial resistome from 79 sites in 60 countries. We find systematic differences abundance and diversity genes between Europe/North-America/Oceania Africa/Asia/South-America. taxonomy only explains minor part variation that observe. no evidence cross-selection...
Background and aimPlasmid-mediated colistin resistance mechanisms have been identified worldwide in the past years. A multiplex polymerase chain reaction (PCR) protocol for detection of all currently known transferable genes (mcr-1 to mcr-5, variants) Enterobacteriaceae was developed surveillance or research purposes. Methods: We designed four new primer pairs amplify mcr-1, mcr-2, mcr-3 mcr-4 gene products used originally described primers mcr-5 obtain a stepwise separation ca 200 bp...
Abstract Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic genes (ARGs) essential develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing analyse ARGs in 757 sewage samples from 243 cities 101 countries, collected 2016 2019. We find regional patterns resistomes, these differ between subsets corresponding drug classes are partly driven by taxonomic variation....
In August 2017, an outbreak of six listeriosis cases in Denmark was traced to cold-smoked salmon, using epidemiological investigations and whole-genome sequencing (WGS) analyses. Exchange genome sequences allowed identification France a food isolate from salmon-derived product human 2016 within the same cgMLST cluster as Danish isolates (L2-SL8-ST8-CT771). The salmon came third European Union country. WGS can rapidly link across Europe.
Abstract The clinical use of the antibiotic erythromycin (ery) is hampered owing to spread resistance genes that are mostly mutating rRNA around ery binding site at entrance protein exit tunnel. Additional effective mechanisms include deletion or insertion mutations in ribosomal uL22, which lead alterations tunnel shape, located 16 Å away from drug’s site. We determined cryo-EM structures Staphylococcus aureus 70S ribosome, and its bound complex with a two amino acid mutation ß hairpin loop,...
Abstract Background Arcobacter spp. have in recent years received increasing interest as potential emerging enteropathogens and zoonotic agents. They are associated with various animals including poultry can be isolated from meat products. The possibilities of persistence cross-contamination slaughterhouses during processing not well established. We evaluated the occurrence a Danish slaughterhouse determined sensitivity isolates to sodium hypochlorite, commonly used biocide. Results...
To characterize the genetic basis of azithromycin resistance in Escherichia coli and Salmonella collected within EU harmonized antimicrobial (AMR) surveillance programme 2014-18 Danish AMR 2016-19.
ABSTRACT Fermented sausages, although presumed safe for consumption, sometimes cause serious bacterial infections in humans that may be deadly. Not much is known about why and when this the case. We tested hypothesis residual veterinary antibiotics meat can disrupt fermentation process, giving pathogenic bacteria a chance to survive multiply. found six commercially available starter cultures were susceptible commonly used antibiotics, namely, oxytetracycline, penicillin, erythromycin. In...
Abstract In Denmark, outbreaks of salmonella with more than 20 cases have become rare. November 2018, an outbreak monophasic Salmonella Typhimurium was detected and investigation initiated the aim identifying source controlling outbreak. Outbreak were defined based on core genome multilocus sequence types. We conducted hypothesis-generating interviews, a matched case-control study, food sampling trace-back investigations. identified 49 distributed across Denmark. univariable analyses...
We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat salt stress of Escherichia coli Salmonella spp. in minced meat reproducibly induced dnaK otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the is applicable studies bacterial gene expression matrix.
Several Proficiency Test (PT) or External Quality Assessment (EQA) schemes are currently available for assessing the ability of laboratories to detect and characterize enteropathogenic bacteria, but they usually targeting one sector, covering either public health, food safety animal health. In addition sector-specific PTs/EQAs detection, cross-sectoral panels would be useful assessment capacity foodborne pathogens in a One Health (OH) perspective further improving interpretation surveillance...
Campylobacter cross-contamination of Danish broiler flocks at slaughterhouses was investigated using data from two national surveillance components and ad-hoc sampling. The animal level (AL) food safety (FS) 2018 were compared. AL component contained results PCR on pools cloacal swabs 3,012 processed (S1-S2), while the FS regarded culture testing leg skins 999/3,012 flocks. monthly "apparent" (AP) "true" flock prevalence (TP) estimated. Agreement between measured in percentage weighted-Kappa...
Listeria monocytogenes is an important human pathogen with a high mortality rate. Consumption of contaminated ready-to-eat food the main mode transmission to humans. Disinfectant-tolerant L. have emerged, which are believed increased persistence potential. Elucidating mechanisms disinfectant tolerance has been focus previous studies using pure cultures. A limitation such approach difficulty identify strains reduced susceptibility due inter-strain variation and need screen large numbers...
This study was conducted to evaluate the performance of a screening protocol detect and isolate mcr-positive Escherichia coli Salmonella spp. from animal caecal content meat samples. We used multicentre approach involving 12 laboratories nine European countries. All participants applied same methodology combining multiplex PCR performed on DNA extracted pre-enrichment step, followed by selective culture step three commercially available chromogenic agar plates. The test panel composed two...
Extended-spectrum β-lactamase- (ESBL) and AmpC- β-lactamase-producing Enterobacterales are widely distributed emerging in both human animal reservoirs worldwide. A growing concern has emerged Europe following the appearance of carbapenemase-producing Escherichia coli (E. coli) primary production food animals. In 2013, European Commission (EC) issued Implementing Decision on monitoring reporting antimicrobial resistance zoonotic commensal bacteria. The Union Reference Laboratory for...
Here, we report on the emergence and spread of multidrug-resistant NDM-1-producing P. aeruginosa isolates from patients hospitalized in Attica region, Greece, 2022 to provide data their resistome, virulome, genetic environment blaNDM-1, molecular epidemiology. A total 17 carbapenem-resistant identified as NDM-producers by immunochromatography at hospital level were sent Central Public Health Laboratory, frame laboratory surveillance pathogens, for further characterization. The initial...
The European Food Safety Authority (EFSA) advised to prioritize monitoring carbapenemase producing Enterobacteriaceae (CPE) in food animals. Therefore, this study evaluated the performance of different commercially available selective agars for detection CPE using spiked pig caecal and turkey meat samples proposed EFSA cultivation protocol. Eleven laboratories from nine countries received eight (four four samples). For each matrix, three contained approximately 100 CFU/g CPE, one sample...