A5065091579- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Peptides and Activities
- Microbial Metabolism and Applications
- Streptococcal Infections and Treatments
- Biochemical and Structural Characterization
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumocystis jirovecii pneumonia detection and treatment
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Infections and bacterial resistance
- Infectious Diseases and Mycology
- Escherichia coli research studies
- Cancer therapeutics and mechanisms
- Bacteriophages and microbial interactions
- Sphingolipid Metabolism and Signaling
- Plant Pathogenic Bacteria Studies
- Diagnosis and treatment of tuberculosis
- Urinary Tract Infections Management
- Pneumonia and Respiratory Infections
Shanghai Pulmonary Hospital
2017-2025
Tongji University
2017-2025
Wenzhou Medical University
2015-2025
First Affiliated Hospital of Wenzhou Medical University
2015-2025
Nanchang University
2015-2024
Fudan University
2010-2022
Center for Discovery
2022
Hackensack Meridian Health
2022
Ningbo First Hospital
2022
Zhejiang University
2013-2019
Since its discovery in the early 2000s, methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) has become a rapidly emerging cause of human infections, most often associated with livestock exposure. We applied whole-genome sequence typing to characterize diverse collection CC398 isolates (n = 89), including MRSA and methicillin-susceptible S. (MSSA) from animals humans spanning 19 countries four continents. identified 4,238 single nucleotide polymorphisms (SNPs) among...
Genetics is a key means to study bacterial physiology. However, the highly desirable scarless genetic manipulation often time-consuming and laborious for major human pathogen K. pneumoniae . We developed CRISPR-Cas9-mediated genome-editing method cytidine base-editing system, enabling rapid, efficient, iterative genome editing in both industrial clinically isolated strains. applied tools dissecting drug resistance mechanism of hypermucoviscous carbapenem-resistant strain, elucidating that...
Hypervirulent and multidrug resistant Klebsiella pneumoniae strains pose a significant threat to the public health. In present study, 21 carbapenem-resistant K. isolates (CRKP) were determined by string test as hypermucoviscous (HMKP), with prevalence of 15.0% (21/140) among CRKP, 1.1% (21/1838) all isolates. Among them, 7 (33.3%), 1 (4.76%) isolate belonged capsular serotype K20 K2 respectively, while 13 (61.9%, 13/21) weren't successfully typed serotyping. All carbapenemase-producers...
A distinctive syndrome caused by hypermucoviscous Klebsiella pneumoniae (HMKP) including pyogenic liver abscess (PLA) is now becoming a globally emerging disease. In the present study, 22.8% (84/369) of K. clinical isolates associated with various types invasive infections were identified as HMKP, 45.2% PLA. Multivariate regression analysis showed that male patients 41-50 years, PLA, diabetes mellitus, and hypertension independent risk factors for HMKP infections. K2 (42.9%, 36/84) was most...
Background: Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide. A major cause for failure cancer therapy development chemoresistance. Although progress has been made in study mechanisms underlying cells resistance, little known about role microRNAs (miRNAs) resistance. Methods and Results: Fifteen miRNAs, including 6 up-regulated miRNAs (> 2.0-fold) 9 down-regulated (< 0.5-fold) were differentially expressed 5-fluorouracil-resistant their parental...
Carbapenem-resistant and hypervirulent Klebsiella pneumoniae strains have emerged recently. These are both multidrug resistant may also be highly transmissible able to cause severe infections in the hospital community.
The aim of this study was to investigate the genomic epidemiology MRSA in China identify predominant lineages and their associated phenotypic characteristics. In study, we conducted whole-genome sequencing on 565 isolates from 7 provinces municipalities between 2014 2020. were subjected MLST, spa typing, SCCmec analysis virulence determinants antimicrobial susceptibility testing. Among tested, clonal complex (CC) 59 (31.2%), CC5 (23.4%) CC8 (13.63%) major lineages, structure dominated by...
Mobile plasmids play a key role in spurring the global dissemination of multidrug-resistant (MDR) K. pneumoniae, while plasmid curing has been recognized as promising strategy to combat antimicrobial resistance. Here we exploited pneumoniae native CRISPR system cure high-risk IncFII plasmids.We examined matched protospacers 725 completely sequenced from genomes. Then, re-engineered CRISPR-Cas3 and deliver via conjugation. Plasmid killing efficiency G. mellonella infection model were applied...
The emergence of antibiotic-resistant and biofilm-producing
Background Long noncoding RNAs (lncRNAs) have been shown to be involved in the development and progression of lung cancer. However, roles lncRNAs cancer are not well understood. Methodology/Principal Findings We used a high-throughput microarray compare lncRNA messenger RNA (mRNA) expression profiles adenocarcinoma normal tissue (NT) samples. Several candidate adenocarcinoma-associated were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis....
To evaluate the diagnostic performance of Xpert MTB/RIF Ultra for EPTB (Extrapulmonary Tuberculosis) patients on different types extrapulmonary specimens from anatomic sites.Patients with suspected were prospectively included, collected and subjected to culture, assays in accordance relevant guidelines.A total 225 cases included which contained 200 (43 culture-positive EPTB, 157 culture-negative diagnosed based pathological results a satisfied response anti-TB treatment) 25 non-EPTB cases....
This study reportedaimed to design a method, called the simplified carbapenem inactivation method (sCIM), for simple and accurate detection of carbapenemase-producing gram-negative bacilli. is based on modified (mCIM) with improvement experimental procedures: Instead incubating antibiotic disk in organism culture media, be test was smeared directly onto disk. carbapenemase phenotypes. ForWe evaluatinged sensitivity specificity A total 196 Enterobacteriaceae, 73 Acinetobacter baumannii, 158...
Staphylococcus aureus sequence type 9 (ST9) is the main LA-MRSA clone spreading in Asian continent. It can colonize and cause mild to severe infections both animal humans.
Klebsiella pneumoniae is one of the most common Gram-negative bacilli isolated from bloodstream infections worldwide, and recently an increased rate carbapenem resistance has been reported in this pathogen. This study aims to describe genomic characteristics carbapenem-resistant K. (CRKP) patients with bacteremia China. We analyzed 147 isolates attended 12 referral hospitals China between April 2015 November 2018. conducted a phenotypic susceptibility evaluation whole genome sequence...
Previous studies have shown that the increased prevalent ST764 clone in China, Japan, and other Asian areas. However, knowledge of genetic features virulence characteristics methicillin-resistant Staphylococcus aureus (MRSA) China is still limited. In this study, we identified 52 ST764-SCCmec type II isolates collected from five cities between 2014 2021. Whole genome sequencing showed most common staphylococcal protein A (spa) types were t002 (55.78%) t1084 (40.38%). Virulence assays...
Fluoroquinolones (FQ) are essential for the treatment of multidrug-resistant tuberculosis (MDR-TB). The FQ resistance (FQ-R) rate in MDR-TB China and its risk factors remain poorly understood. We conducted a retrospective, population-based genomic epidemiology study patients Shanghai, China, from 2009 to 2018. A cluster was defined as strains with genetic distances ≤ 12 single nucleotide polymorphisms. transmitted FQ-R same resistance-conferring mutations shared by ≥ 2 cluster. used...
Abstract Background Staphylococcus aureus , particularly methicillin-resistant S. (MRSA), is an important cause of pyogenic skin and soft tissue infections (SSTIs). The aim present study to investigate the molecular characteristic isolates isolated from pus samples patients with purulent in Wenzhou, China. Methods Between December 2002 June 2008, a total 111 nonduplicate were collected SSTIs teaching hospital All tested confirmed as using Staph SPA agglutination kit, Gram's stain Vitek-60...