A5033027758- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Vector-borne infectious diseases
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- Bacterial Infections and Vaccines
- Viral Infections and Vectors
- Bacterial biofilms and quorum sensing
- Syphilis Diagnosis and Treatment
- Infective Endocarditis Diagnosis and Management
- Tuberculosis Research and Epidemiology
- Respiratory viral infections research
- Microbial infections and disease research
- Orthopedic Infections and Treatments
- Genomics and Phylogenetic Studies
- Vector-Borne Animal Diseases
- Virology and Viral Diseases
- Reproductive tract infections research
- Bartonella species infections research
- Plant Pathogenic Bacteria Studies
- Yersinia bacterium, plague, ectoparasites research
- Pneumocystis jirovecii pneumonia detection and treatment
- Vibrio bacteria research studies
National Institute for Public Health and the Environment
2015-2024
Netherlands Food and Consumer Product Safety Authority
2021
Wilhelmina Children's Hospital
2010
University Medical Center Groningen
2010
University of Würzburg
2010
Amsterdam UMC Location University of Amsterdam
2010
Institute of Environmental Science and Research
2010
Newron Pharmaceuticals (Italy)
2010
Novartis (Italy)
2010
University Medical Center Utrecht
2010
Widespread use of DNA restriction fragment length polymorphism (RFLP) to differentiate strains Mycobacterium tuberculosis monitor the transmission has been hampered by need culture this slow-growing organism and level technical sophistication needed for RFLP typing. We have developed a simple method which allows simultaneous detection typing M. in clinical specimens reduces time between suspicion disease from 1 or several months 3 days. The is based on chromosomal DR locus, contains variable...
Summary Using in silico analysis we studied a novel family of repetitive DNA sequences that is present among both domains the prokaryotes (Archaea and Bacteria), but absent from eukaryotes or viruses. This characterized by direct repeats, varying size 21 to 37 bp, interspaced similarly sized non‐repetitive sequences. To appreciate their characteri‐stic structure, will refer this as clustered regularly short palindromic repeats (CRISPR). In most species with two more CRISPR loci, these loci...
ABSTRACT In this study, we developed a simple and fast typing procedure for 37 mucosotropic human papillomavirus (HPV) types using nonradioactive reverse line blotting (RLB) general primer (GP5+/6+) PCR products. This system has the advantages not only that in format, up to 42 products can be simultaneously typed per membrane day, but also after stripping, membranes easily rehybridized at least 15 times without loss of signal. RLB appeared highly specific, its sensitivity was identical...
ABSTRACT A reverse line blot (RLB) assay was developed for the identification of cattle carrying different species Theileria and Babesia simultaneously. We included annulata , T. parva mutans taurotragi velifera in assay, as well parasites belonging to sergenti-T. buffeli-T. orientalis group. The were bovis B. bigemina divergens . employs one set primers specific amplification rRNA gene V4 hypervariable regions all species. PCR products obtained from blood samples hybridized a membrane onto...
A sensitive and specific PCR hybridization assay was developed for the simultaneous detection identification of Ehrlichia Borrelia burgdorferi sensu lato. In separate assays 16S rRNA gene species 23S-5S spacer region B. lato were amplified labeled by PCR. These products used in a reverse line blot which oligonucleotide probes are covalently linked to membrane parallel lines. Hybridization samples with on this enabled Ehrlichia, burgdorferi, Bartonella 40 different samples. The application...
A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR genes coding for carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of encoded various species Enterobacteriaceae (e.g., Klebsiella pneumoniae, Escherichia coli Enterobacter cloacae), but also non-fermenters Pseudomonas aeruginosa...
ABSTRACT The implementation of routine whole-genome sequencing (WGS) promises to transform our ability monitor the emergence and spread bacterial pathogens. Here we combined WGS data from 308 invasive Staphylococcus aureus isolates corresponding a pan-European population snapshot, with epidemiological resistance data. Geospatial visualization is made possible by generic software tool designed for public health purposes that available at project URL (...
In an attempt to characterize unusual mycobacterial strain isolated from a 2-year-old Somali patient with lymphadenitis, we applied various molecular methods not previously used for the taxonomic classification of mycobacteria. This isolate, designated So93, did differ Mycobacterium tuberculosis in biochemical tests and its 16S rRNA sequence, but produced smooth glossy colonies, which is highly exceptional this species. phenotype was unstable switched nonreversibly rough colony morphology...
The continuous presence of bacteria or their degraded antigens in the synovium may be involved pathogenesis rheumatoid arthritis (RA). aim this study was to determine bacterial nucleic acids and cell wall constituents joints patients with RA other forms arthritis.Joint samples were obtained from (n = 26), septic 2), inflammatory osteoarthritis 5), gout 6), joint trauma 1). Universal 16S-ribosomal RNA primers used detect DNA these samples, using stringent regimens for sample collection...
We developed a rapid and reliable method for the identification Borrelia burgdorferi sensu lato species in ticks. used DNA sequence polymorphism of spacer region between 5S 23S rRNA genes, which has been shown to be able discriminate eight genomic groups B. (D. Postic, M. Assous, P. A. D. Grimont, G. Baranton, Int. J. Syst. Bacteriol. 44:743-752, 1994). Spacer was amplified by PCR then hybridized five membrane-bound oligonucleotides. The oligonucleotides were specific stricto, garinii,...
Bordetella pertussis, B. bronchiseptica, parapertussis(hu), and parapertussis(ov) are closely related respiratory pathogens that infect mammalian species. pertussis parapertussis(hu) exclusively human cause whooping cough, or a disease has resurged despite vaccination. Although it most often infects animals, infrequently bronchiseptica is isolated from humans, these infections thought to be zoonotic. assumed have evolved bronchiseptica-like ancestor independently. To determine the...
ABSTRACT The direct repeat region in Mycobacterium tuberculosis complex strains is composed of multiple variant repeats (DVRs), each which a 36-bp (DR) plus nonrepetitive spacer sequence similar size. It has been shown previously that clinical isolates show extensive polymorphism the DR by variable presence DVRs, and this used epidemiology tuberculosis. In an attempt to better understand evolutionary scenario leading polymorphic loci improve strain differentiation spoligotyping, we...
ABSTRACT Three molecular typing methods were used to study the relationships among 184 Campylobacter strains isolated from humans, cattle, and chickens. All genotyped by amplified fragment length polymorphism (AFLP) analysis, multilocus sequence (MLST), analysis of a genomic region with short tandem repeats designated clustered regularly interspaced palindromic (CRISPRs). MLST AFLP yielded more than 100 different profiles patterns, respectively. These multiple-locus resulted in similar...
ABSTRACT The presence and distribution of Ehrlichia spp. Borrelia burgdorferi sensu lato was demonstrated among ixodid ticks collected in the Baltic regions Russia, where Lyme borreliosis is endemic. A total 3,426 Ixodes ricinus 1,267 persulcatus specimens were collected, dark-field microscopy showed that 265 (11.5%) I. 333 (26.3%) positive. From these samples, 472 dark-field-positive 159 dark-field-negative subjected to PCR subsequent reverse line blot hybridization. Fifty-four (8.6%)...
The diagnostic value of the detection immunoglobulin G (IgG) and IgM by Bartonella henselae-based indirect fluorescence assay (IFA) enzyme-linked immunoassay (EIA) for diagnosis cat scratch disease (CSD) was evaluated. IFA performed either with B. henselae that cocultivated a few hours Vero cells or noncocultivated as antigen. Additionally, performance PCR hybridization based on 16S rRNA gene determined compared those serologic assays. study group consisted 45 patients suspected suffering...
The X region of the protein A gene Staphylococcus aureus contains a highly polymorphic sequence which is composed repeats 24 bp. We used amplification by PCR to investigate whether this could be discriminate between epidemic and nonepidemic methicillin-resistant S. (MRSA) strains. Most MRSA strains (24 33) harbored more than seven repeats, while most (10 14) contained or fewer repeats. It conceivable that longer results in better exposition Fc-binding A, thereby facilitating colonization...
Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) is required to study the routes and rates transmission this pathogen. Currently available techniques are either resource-intensive or have limited discriminatory ability. Multiple-locus variable number tandem repeat analysis (MLVA) may provide an alternative high throughput molecular tool with epidemiological resolution.A new MLVA scheme for S. was validated using 1681 isolates collected from Dutch patients 100 pigs. 8...
A gene bank of Mycobacterium bovis BCG DNA in Escherichia coli was constructed by cloning Sau3A-cleaved mycobacterium fragments into the lambda vector EMBL3. The expression mycobacterial antigens analyzed Western blotting with hyperimmune rabbit sera. Among 770 clones tested, several were found that produced various low amounts, concentrations generally close to detection limit. One particular clone chosen for further investigation. This a 64-kilodalton (kDa) antigen. By placing promoter PL...
Abstract In the Netherlands, 7-valent pneumococcal conjugate vaccine (PCV-7) was implemented in a 3+1-dose schedule national immunization program for infants born after April 1, 2006. To assess vaccine's effectiveness, we compared disease incidence before and implementation (June 2004–June 2006 June 2006–June 2008, respectively). We serotyped 2,552 invasive isolates from throughout covering 25% of country's population. Clinical characteristics were extracted hospital records. After 2006,...
>Staphylococcus aureus is one of the most important human pathogens and meticillin-resistant S. (MRSA) presents a major cause healthcare- community-acquired infections. This study investigated spatial temporal changes causing bacteraemia in Europe over five-year interval explored possibility integrating pathogen-based typing data with epidemiological clinical information at European level. Between January 2011 July 2011, 350 laboratories serving 453 hospitals 25 countries collected 3,753...
Carbapenem-hydrolysing enzymes belonging to the OXA-48-like group are encoded by bla OXA-48 -like alleles and abundant among Enterobacterales in Netherlands. Therefore, objective here was investigate characteristics, gene content diversity of carrying plasmids chromosomes Escherichia coli Klebsiella pneumoniae collected Dutch national surveillance from 2014 2019 comparison with genome sequences 29 countries. A combination short-read sequencing long-read enabled reconstruction 47 132 complete...
Restriction endonuclease analysis of the PCR-amplified 16S-23S rRNA gene spacer region was used to investigate prevalence Bartonella henselae variants in samples from cat-scratch disease (CSD) patients. Analysis PCR fragments 27 DNA-positive Dutch patients with CSD AluI revealed two restriction fragment length polymorphism (RFLP) patterns, patterns A and B. Twenty yielded pattern A, 7 Three North American were shown contain RFLP To be able detect differentiate DNA clinical material more...