A5073692406- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Use and Resistance
- SARS-CoV-2 and COVID-19 Research
- Mycobacterium research and diagnosis
- Viral Infections and Vectors
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Animal Virus Infections Studies
- Microbial Metabolism and Applications
- Ocular Infections and Treatments
- Rabies epidemiology and control
- COVID-19 Clinical Research Studies
- Zoonotic diseases and public health
- Brucella: diagnosis, epidemiology, treatment
- Milk Quality and Mastitis in Dairy Cows
- Gut microbiota and health
- Leptospirosis research and findings
- Veterinary medicine and infectious diseases
- Nail Diseases and Treatments
- Animal Behavior and Welfare Studies
Utrecht University
2016-2025
European Society of Clinical Microbiology and Infectious Diseases
2024
Wageningen University & Research
2008-2023
Tokai University
2023
Erasmus MC
2023
Ministry of Agriculture, Nature and Food Quality
2021
Netherlands Food and Consumer Product Safety Authority
2021
National Institute for Public Health and the Environment
2008-2011
National Institute Of Veterinary Epidemiology And Disease Informatics
2008-2009
Extended-spectrum-β-lactamase (ESBL)/AmpC producing Enterobacteriaceae have been reported worldwide amongst isolates obtained from humans, food-producing animals, companion and environmental sources. However, data on prevalence of fecal carriage ESBL/AmpC in healthy animals is limited. This pilot study describes the encoding genes cats dogs, dogs with diarrhea. Twenty samples each group were cultured MacConkey agar supplemented 1 mg/L cefotaxime LB-enrichment broth cefotaxime, which was...
The close contact between household pets and people offers favourable conditions for bacterial transmission. In this article, the aetiology, prevalence, transmission, impact on human health preventative measures are summarized selected zoonoses transmissible by pets. Six representing distinct transmission routes were arbitrarily based available information incidence severity of pet-associated disease caused zoonotic bacteria: bite infections cat scratch (physical injuries), psittacosis...
We compared the prevalence of human and animal methicillin-resistant Staphylococcus aureus (MRSA) at pig farms in The Netherlands, related this to individual farm-level characteristics. More than half investigated (28/50) had MRSA pigs or stable dust about one third (15/50) person(s) were identified as carriers. Human carriage was found only on with MRSA-positive dust. strains samples same spa-type all not typable by pulsed-field gel electrophoresis (NT-MRSA). Multivariate analyses showed...
Livestock-associated MRSA has been found in various animals, livestock farmers and retail meat. This study aimed to determine the prevalence determinants of nasal carriage pig slaughterhouse workers. Three large slaughterhouses The Netherlands were studied 2008 using human environmental samples. overall employees was 5.6% (14/249) (95% CI 3.4-9.2) working with live pigs single most important factor for being positive (OR 38.2, P<0.0001). At start day only samples from lairages (10/12),...
Animals like mink, cats and dogs are susceptible to SARS-CoV-2 infection. In the Netherlands, 69 out of 127 mink farms were infected with between April November 2020 all on culled after infection prevent further spread virus. On some farms, (feral) present. This study provides insight into prevalence SARS-CoV-2-positive in 10 their possible role transmission Throat rectal swabs 101 (12 domestic 89 feral cats) 13 tested for using PCR. Serological assays performed serum samples from 62 adult...
There is a growing concern regarding the increase of antimicrobial resistant bacteria in companion animals. Yet, there are no studies comparing resistance levels these organisms European countries. The aim this study was to investigate geographical and temporal trends causing urinary tract infection (UTI) animals Europe. susceptibility 22 256 isolated from dogs cats with UTI determined. Samples were collected between 2008 2013 16 laboratories 14 prevalence most common determined for each...
Antimicrobial resistance (AMR) should be tackled through a One Health approach, as stated in the World Organization Global Action Plan on AMR. We describe landscape of AMR surveillance European Union/European Economic Area (EU/EEA) and underline gap regarding veterinary medicine. Current efforts are limited help to practitioners policymakers seeking improve antimicrobial stewardship animal health. propose establish Resistance Surveillance network Veterinary medicine (EARS-Vet) report...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect many animal species, including minks, cats, and dogs. To gain insights into SARS-CoV-2 infections in cats dogs, we developed validated a set of serologic assays, ELISA virus neutralization. Evaluation samples from animals before they acquired disease roaming SARS-CoV-2-positive mink farms confirmed the suitability these assays for specific antibody detection. Furthermore, our findings exclude nucleocapsid protein as an...
Several domestic and wild animal species are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Reported (sero)prevalence in dogs cats vary largely depending on the target population, test characteristics, geographical location time period. This research assessed prevalence of SARS-CoV-2-positive (PCR- and/or antibody positive) two different populations. Dogs living a household with at least one confirmed COVID-19-positive person (household (HH) study; 156...
ABSTRACT A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity were shown by evaluating the test on a large strain collection ( n = 1,307). We recommend this accurate, rapid, inexpensive diagnosis methicillin-resistant S. (MRSA) CC398 in hospitals farms.
Methicillin-resistant Staphylococcus aureus clonal complex (CC) 398 has emerged from pigs to cause human infections in Europe and North America. We used a new 62-strain S. microarray (SAM-62) compare genomes of isolates three geographical areas (Belgium, Denmark, Netherlands) understand how CC398 colonizes different mammalian hosts. The core 44 pig 32 humans did not vary. However, mobile genetic element (MGE) distribution was variable including SCCmec. φ3 bacteriophage specificity genes...
Methicillin-resistant Staphylococcus pseudintermedius (MRSP), which is often multidrug resistant (MDR), has recently emerged as a threat to canine health worldwide. Knowledge of the temporal distribution specific MRSP lineages, their antimicrobial resistance phenotypes, and association with clinical conditions may help us understand emergence spread in dogs. The aim this study was determine yearly proportions lineages antimicrobial-resistant phenotypes Netherlands examine possible...
Canine atopic dermatitis is a genetically predisposed inflammatory and pruritic allergic skin disease that often complicated by (secondary) bacterial fungal (yeast) infections. High-throughput DNA sequencing was used to characterize the composition of microbiome (bacteria fungi) inhabiting specific sites in healthy dogs with (AD) before after topical antimicrobial treatment. Skin samples were collected from six control three spontaneously affected AD swabbing at (non-) predilection before,...
Antimicrobial stewardship guidelines (ASGs) represent an important tool to help veterinarians optimize their antimicrobial use with the objective of decreasing resistance. The aim this study was map and qualitatively assess ASGs for in cats dogs Europe. Country representatives European Network Optimization Veterinary Treatment (ENOVAT) were asked identify published countries. All collated updated since January 2010 containing recommendations on therapy at least three conditions affecting...
The monitoring of antimicrobial resistance (AMR) in bacterial pathogens animals is not currently coordinated at European level. To fill this gap, experts the Union Joint Action on Antimicrobial Resistance and Healthcare Associated Infections (EU-JAMRAI) recommended building Surveillance network Veterinary medicine (EARS-Vet). In study, we (i) identified national systems for AMR (both companion food-producing) among 27 countries affiliated to EU-JAMRAI, (ii) described their structures...
Since the detection of MRSA CC398 in pigs 2004, it has emerged livestock worldwide. been found people contact with and thus become a public health issue. Data from large-scale longitudinal study two Danish four Dutch pig herds were used to quantify transmission rates within identify factors affecting between pigs. Sows their offspring sampled at varying intervals during production cycle. Overall prevalence sows increased 33% before farrowing 77% weaning. piglets was > 60% entire period. The...
ABSTRACT A longitudinal study was performed to (i) investigate the continuity of shedding extended-spectrum-beta-lactamase (ESBL)-producing Enterobacteriaceae in dogs without clinical signs, (ii) identify dominant plasmid-mediated ESBL genes, and (iii) quantify ESBL-producing feces. Fecal samples from 38 were collected monthly for 6 months. Additional 7 included on a weekly basis weeks. Numbers CFU per gram feces non-wild-type determined by using MacConkey agar supplemented with 1 mg/liter...
Close contact between pets and owners provides the opportunity for transmission of antimicrobial resistant organisms like extended-spectrum beta-lactamase (ESBL)/AmpC (AmpC)-producing Enterobacteriaceae, posing a risk to public health.To investigate whether raw feed is factor household cats shed ESBL-producing cohort study was designed. Additionally, non-raw commercial pet food products were screened presence Enterobacteriaceae.Weekly fecal samples 17 in control group 19 exposed collected...
In the Netherlands, 97 human leptospirosis cases were notified in 2014. This represents a 4.6-fold increase autochthonous (n = 60) compared with annual average between 2010 and 2013. Most had symptom onset June November. marked humans coincided an of dogs. 2014, 13 dogs reported, two to six annually from The majority 20) linked recreational exposure, e.g. swimming or fishing, followed by occupational exposure 15). About sixty per cent 37) most likely attributable surface water contact,...
Staphylococcus aureus is a contagious, opportunistic pathogen that causes clinical or subclinical mastitis in dairy cattle. The genetic background and antimicrobial resistance of isolates from Ethiopian farms has not been studied. Therefore, the aim this study was to characterize S. hand milked cows, by spa, MLST virulence factor typing, assessment susceptibility. A total 79 intramammary infections PCR used detect lukM-lukF' pvl genes encoding bovine human associated bi-component...
ABSTRACT Fifteen percent of all methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) human carriers detected in The Netherlands had not been direct contact with pigs or veal calves. To ensure low MRSA prevalence, it is important to investigate the likely origin this unknown (MUO). Recently, was shown that CC398 strains originating from humans and animals differ presence specific mobile genetic elements (MGEs). We hypothesized determining these MGEs MUO isolates...