A5009607061- Neurofibromatosis and Schwannoma Cases
- Stellar, planetary, and galactic studies
- Astronomy and Astrophysical Research
- Neuroblastoma Research and Treatments
- Hippo pathway signaling and YAP/TAZ
- Axon Guidance and Neuronal Signaling
- Astronomical Observations and Instrumentation
- Meningioma and schwannoma management
- Proteoglycans and glycosaminoglycans research
- Signaling Pathways in Disease
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Astrophysics and Star Formation Studies
- Galaxies: Formation, Evolution, Phenomena
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Peptidase Inhibition and Analysis
- History and Developments in Astronomy
- Immune cells in cancer
- Protein Tyrosine Phosphatases
- Hereditary Neurological Disorders
- Bone Tumor Diagnosis and Treatments
- Neuroscience and Neuropharmacology Research
- Spectroscopy and Laser Applications
- Melanoma and MAPK Pathways
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2016-2025
Friedrich Schiller University Jena
2020-2024
Jena University Hospital
2019
University of California, San Francisco
2016
Leibniz Association
2008-2015
Case Western Reserve University
2000-2014
Newcastle University
2010-2013
National Institutes of Health
2010
National Center on Birth Defects and Developmental Disabilities
2010
FZI Research Center for Information Technology
2000-2007
The neurofibromatosis-2 ( NF2 ) gene encodes merlin, an ezrin-radixin-moesin-(ERM)-related protein that functions as a tumor suppressor. We found merlin mediates contact inhibition of growth through signals from the extracellular matrix. At high cell density, becomes hypo-phosphorylated and inhibits in response to hyaluronate (HA), mucopolysaccharide surrounds cells. Merlin's growth-inhibitory activity depends on specific interaction with cytoplasmic tail CD44, transmembrane HA receptor. low...
Abstract The small G-protein Ras is a tightly controlled regulator of cell fate. Prolonged or persistent arrest in the activated GTP-loaded state by mutation as lung cancer Ras–GTPase-activating protein neurofibromatosis type 1 promotes tumorigenesis. We now show that tumor-suppressor merlin (mutated 2) also controls activity. Systematic analysis growth factor signaling located step interference to activation and Rac. Merlin independently uncouples both Rac from signals. In case Ras, acts...
In several types of cells, the activation receptor tyrosine kinase c-Met by its ligand hepatocyte growth factor (HGF) requires coreceptor CD44v6. The CD44 extracellular domain is necessary for autophosphorylation, whereas intracellular required signal transduction. We have already shown that cytoplasmic tail recruits ezrin, radixin and moesin (ERM) proteins to complex CD44v6, c-Met, HGF. now defined function ERM step they promote in signaling cascade. association absolutely mediate...
In childhood B-cell precursor acute lymphoblastic leukemia, cytogenetics is important in diagnosis and as an indicator of response to therapy, thus playing a key role risk stratification patients for treatment. Little known the relationship between different cytogenetic subtypes leukemia recently reported copy number abnormalities affecting significant associated genes. consecutive series 1427 patients, we have determined incidence type using multiplex ligation-dependent probe amplification....
Abstract The regenerative capacity of peripheral nerves declines during aging, contributing to the development neuropathies, limiting organism function. Changes in Schwann cells prompt failures instructing maintenance and regeneration aging nerves; molecular mechanisms which have yet be delineated. Here, we identified an altered inflammatory environment leading a defective cell response, as underlying mechanism impaired nerve aging. Chronic inflammation was detected intact uninjured old...
Loss of the Merlin tumor suppressor and activation Hippo signaling pathway play major roles in control cell proliferation tumorigenesis. We have identified completely novel for effector Yes-associated protein (YAP) Schwann (SC) plasticity peripheral nerve repair after injury. Injury to nervous system (PNS) causes a dramatic shift SC molecular phenotype generation repair-competent SCs, which direct functional repair. find that loss these cells catastrophic failure axonal regeneration...
Abstract Recent genomic studies have shown that copy number abnormalities (CNA) of genes involved in lymphoid differentiation and cell cycle control are common B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL). We evaluated Multiplex Ligation‐dependent Probe Amplification (MLPA) on 43 BCP‐ALL patients for the detection most deletions among these compared results to those obtained by fluorescence situ hybridization (FISH) quantitative PCR (qPCR). There was good correlation between...
Schwannomas are predominantly benign nerve sheath neoplasms caused by Nf2 gene inactivation. Presently, treatment options mainly limited to surgical tumor resection due the lack of effective pharmacological drugs. Although mechanistic understanding function has advanced, it so far been primarily restricted Schwann cell-intrinsic events. Extracellular cues determining cell behavior with regard schwannoma development remain unknown. Here we show pro-tumourigenic microenvironmental effects on...
We analyze a sample of tens thousands spectra halo turnoff stars, obtained with the optical spectrographs Sloan Digital Sky Survey (SDSS), to characterize stellar population "in situ" out distance few kpc from Sun. In this paper we describe derivation atmospheric parameters. also derive overall metallicity distribution based on F-type stars observed as flux calibrators for Baryonic Oscillations Spectroscopic (BOSS). Our analysis is an automated method that determines set parameters model...
We have investigated the immunohistochemical expression of p53 protein in 96 cases non‐Hodgkin's lymphoma using a panel five antibodies. Positive neoplastic cells were found 30 (31.2%) cases, which could be divided into two groups according to their patterns reactivity with different antibodies; i.e. those positive both polyclonal and monoclonal antibodies, stained only by antibodies PAb1801 and/or PAb240. Positivity was nuclear all but six cytoplasmic staining found. In view hypothesis...
Inactivation of the tumor suppressor NF2/merlin underlies neurofibromatosis type 2 (NF2) and some sporadic tumors. Previous studies have established that merlin mediates contact inhibition proliferation; however, exact mechanisms remain obscure multiple pathways been implicated. We previously reported inhibits Ras Rac activity during inhibition, but how regulates has remained elusive. Here we demonstrate can directly interact with both p120RasGAP (also named RasGAP). While does not increase...
Background & Aims: Dysregulation of metabolism plays an important role in the development and progression cancers, while underlying mechanisms remain largely unknown.This study aims to explore regulation relevance glycolysis chemoresistance gastric cancer.Methods: Biochemical differences between chemoresistant chemosensitive cancer cells were determined by profiling, microarray gene expression, PCR or western blotting.Cancer cell growth vitro vivo analyzed viability, apoptosis nude mice...
Neurofibromatosis Type 2 (NF2) is an autosomal dominant genetic syndrome caused by mutations in the NF2 tumor suppressor gene resulting multiple schwannomas and meningiomas. There are no FDA approved therapies for these tumors their relentless progression results high rates of morbidity mortality. Through a combination throughput screens, preclinical vivo modeling, evaluation kinome en masse , we identified actionable drug targets efficacious experimental therapeutics treatment related These...
Osteosarcoma is the most common type of pediatric bone tumor. Despite great advances in chemotherapy during past decades, survival rates osteosarcoma patients remain unsatisfactory. Drug resistance one main reasons, leading to treatment failure and poor prognosis. Previous reports correlated expression cluster differentiation 44 (CD44) with drug patients, however underlying mechanisms are poorly defined. Here, we investigated role CD44 regulation chemoresistance, using cells isolated from...