A5066150732- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Neurofibromatosis and Schwannoma Cases
- Bacterial Genetics and Biotechnology
- Estrogen and related hormone effects
- RNA modifications and cancer
- Neuroblastoma Research and Treatments
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Virus-based gene therapy research
- Sarcoma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Cells and Metastasis
- Carcinogens and Genotoxicity Assessment
- NF-κB Signaling Pathways
- Testicular diseases and treatments
- Cancer-related gene regulation
- Urologic and reproductive health conditions
- Monoclonal and Polyclonal Antibodies Research
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2014-2023
Institute of Genetics
2017
FZI Research Center for Information Technology
2000-2016
Leibniz Association
2009-2015
Institute of Cytology
2014
St Petersburg University
2014
BP (United Kingdom)
2013
Karlsruhe Institute of Technology
1995-2010
State Museum of Natural History Karlsruhe
1993-2007
Ludwig Cancer Research
2003
Genomic clones coding for human fibroblast collagenase were isolated. By constructing and transfecting mutants with 5' 3' deletion mutations of the control region gene into or murine cells, we delimited a 32-base-pair sequence at positions -73 to -42 which is required induction transcription by tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. The DNA element behaves as 12-O-tetradecanoyl-phorbol-13-acetate-inducible enhancer: it mediates stimulation heterologous herpes simplex virus...
UV irradiation of human and murine cells enhances the transcription several genes. Here we report on primary target relevant absorption, pathways leading to gene activation, elements receiving UV-induced signal in immunodeficiency virus type 1 (HIV-1) long terminal repeat, coding for collagenase, cellular oncogene fos. In order induce expression radiation needs be absorbed by DNA cause damage kind that cannot repaired from patients with xeroderma pigmentosum group A. activation three genes...
The neurofibromatosis-2 ( NF2 ) gene encodes merlin, an ezrin-radixin-moesin-(ERM)-related protein that functions as a tumor suppressor. We found merlin mediates contact inhibition of growth through signals from the extracellular matrix. At high cell density, becomes hypo-phosphorylated and inhibits in response to hyaluronate (HA), mucopolysaccharide surrounds cells. Merlin's growth-inhibitory activity depends on specific interaction with cytoplasmic tail CD44, transmembrane HA receptor. low...
The tyrosine kinase receptor c-Met and its ligand HGF/SF, ezrin, splice variants of CD44 have independently been identified as tumor metastasis-associated proteins. We now show that these proteins cooperate. A isoform containing variant exon v6 sequences is strictly required for activation by HGF/SF in rat human carcinoma cells, established cell lines well primary keratinocytes. CD44v6-deficient cells were unable to activate unless they transfected with a CD44v6-bearing isoform. Antibodies...
A comparison of the cDNA-derived amino acid sequences human stromelysin and collagenase with N-terminal purified enzymes reveals that these metalloproteinases are highly conserved they secreted as proenzymes. putative zinc-binding site was identified by its homology zinc-chelating sequence thermolysin. These permitted identification of: transin, a protein induced in rat fibroblasts either exposed to growth factors or transformed oncogenic viruses, homologue stromelysin, XHF1, after treatment...
A variant of the glycoprotein CD44 (CD44v) that shares sequences with variants causally involved in metastasis formation is transiently expressed on B and T lymphocytes macrophages after antigenic stimulation postnatal period. Antibodies to hinder vivo activation both cells. The observation a protein domain required for lymphatic spread tumor cells can catalyze an essential step process lymphocyte supports idea metastasizing mimic behavior.
A recently described splice variant of CD44 expressed in metastasizing cell lines rat tumors has been shown to confer metastatic potential a non-metastasizing pancreatic carcinoma line and sarcoma cells. Homologues this as well several other variants are also at the RNA level human from lung, breast, colon, immortalized keratinocytes. Using antibodies raised against bacterial fusion protein encoded by sequences, we studied expression glycoproteins normal tissues colorectal neoplasia....
A splice variant of CD44 (CD44v) originally discovered on metastases a rat pancreatic adenocarcinoma (BSp73ASML) has been shown by transfection to confer metastatic behavior nonmetastatic tumor cells (Günthert U., M. Hofmann, W. Rudy, S. Reber, Zöller, I. Haussmann, Matzku, A. Wenzel, H. Ponta, and P. Herrlich. 1991. Cell. 65:13). monoclonal antibody (mAb), 1.1ASML, the metastasis-specific domain CD44v molecule retards growth lymph node lung line BSp73ASML, can efficiently prevent formation...
A recently described splice variant of CD44 expressed in metastasizing cell lines rat tumors, has been shown to confer metastatic potential nonmetastasizing pancreatic carcinoma and sarcoma lines. Using antibodies raised against a bacterial fusion protein encoded by sequences, we have explored the expression glycoproteins on human lymphoid cells tissues non-Hodgkin's lymphomas. Normal lymphohematopoietic express barely detectable low levels glycoproteins, whereas T lymphocytes, upon...