A5047751070- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Bacterial Genetics and Biotechnology
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Coastal wetland ecosystem dynamics
- Plant Pathogenic Bacteria Studies
- Advanced biosensing and bioanalysis techniques
- Wastewater Treatment and Nitrogen Removal
- Nematode management and characterization studies
- Bacteriophages and microbial interactions
- Biofuel production and bioconversion
- Vibrio bacteria research studies
- CRISPR and Genetic Engineering
- Magnetic properties of thin films
- Microbial Metabolic Engineering and Bioproduction
- Polysaccharides and Plant Cell Walls
- RNA and protein synthesis mechanisms
- Characterization and Applications of Magnetic Nanoparticles
- Cell Image Analysis Techniques
- Bacterial Infections and Vaccines
- Bacterial biofilms and quorum sensing
- Agronomic Practices and Intercropping Systems
- Force Microscopy Techniques and Applications
Loewe Center for Synthetic Microbiology
2016-2025
Philipps University of Marburg
2016-2025
Heinrich Heine University Düsseldorf
2021
Kiel University
2020
Deltares
2018
University of Freiburg
2008-2013
Bielefeld University
2000-2010
GTx (United States)
2005-2008
University of Lausanne
2007
Laboratoire des Interactions Plantes Micro-Organismes
2004
The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain compounds by bacterial reduction dinitrogen (N 2 ) ammonium (NH 4 + ). We present here the annotated DNA sequence α-proteobacterium Sinorhizobium meliloti , symbiont alfalfa. tripartite 6.7-megabase (Mb) genome comprises a 3.65-Mb chromosome, and 1.35-Mb pSymA 1.68-Mb pSymB megaplasmids. Genome analysis indicates that all three elements contribute, in...
Fast and reliable detection of patients with severe heterogeneous illnesses is a major goal precision medicine1,2. Patients leukaemia can be identified using machine learning on the basis their blood transcriptomes3. However, there an increasing divide between what technically possible allowed, because privacy legislation4,5. Here, to facilitate integration any medical data from owner worldwide without violating laws, we introduce Swarm Learning-a decentralized machine-learning approach that...
The whole-genome-sequenced rhizobacterium Bacillus amyloliquefaciens FZB42(T) (Chen et al., 2007) and other plant-associated strains of the genus described as belonging to species or subtilis are used commercially promote growth improve health crop plants. Previous investigations revealed that a group represented distinct ecotype related B. amyloliquefaciens; however, exact taxonomic position this remains elusive (Reva 2004). In present study, we demonstrated ability closely strain colonize...
Abstract The extensive information capacity of DNA, coupled with decreasing costs for DNA synthesis and sequencing, makes an attractive alternative to traditional data storage. processes writing, storing, reading exhibit specific error profiles constraints sequences have adhere to. We present DNA-Aeon, a concatenated coding scheme It supports the generation variable-sized encoded user-defined Guanine-Cytosine (GC) content, homopolymer length limitation, avoidance undesired motifs. further...
Sinorhizobium meliloti is an α-proteobacterium that forms agronomically important N 2 -fixing root nodules in legumes. We report here the complete sequence of largest constituent its genome, a 62.7% GC-rich 3,654,135-bp circular chromosome. Annotation allowed assignment function to 59% 3,341 predicted protein-coding ORFs, rest exhibiting partial, weak, or no similarity with any known sequence. Unexpectedly, level reiteration within this replicon low, only two genes duplicated more than 90%...
Abstract Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting various stress conditions. In this study, we focused on the overlapping genetic program activated by two commonly studied in addition to identifying AM-related genes. We thus applied 16,086 probe microarrays profile transcriptome of model legume Medicago truncatula during interactions with Glomus mosseae intraradices...
Analysis of the 1,683,333-nt sequence pSymB megaplasmid from symbiotic N 2 -fixing bacterium Sinorhizobium meliloti revealed that replicon has a high gene density with total 1,570 protein-coding regions, few insertion elements and regions duplicated elsewhere in genome. The only copies an essential arg-tRNA minCDE genes are located on pSymB. Almost 20% carries encoding solute uptake systems, most which were ATP-binding cassette family. Many previously unsuspected involved polysaccharide...
Abstract In this study, we describe a large-scale expression-profiling approach to identify genes differentially regulated during the symbiotic interaction between model legume Medicago truncatula and nitrogen-fixing bacterium Sinorhizobium meliloti. Macro- microarrays containing about 6,000 probes were generated on basis of three cDNA libraries dedicated study root interactions. The experiments performed wild-type mutant material led us set 756 either up- or down-regulated at different...
Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or the rhizosphere of plants and symbiotic within host plant cells, where bacteria ultimately differentiate into nitrogen-fixing organelle-like called bacteroids. As step toward understanding physiology S. its forms transition two, gene expression profiles were determined under two sets biological conditions: growth oxic versus microoxic conditions, state. Data acquisition was based on...
ABSTRACT Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It composed of polymerized pentasaccharide repeating units which are assembled sequential addition glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose on a polyprenol phosphate carrier (L. Ielpi, R. O. Couso, M. A. Dankert, J. Bacteriol. 175:2490–2500, 1993). A cluster 12 genes in region designated xpsI or gum has been...
Plants have developed a wide-range of adaptations to overcome nutrient limitation, including changes the quantity and composition carbon-containing compounds released by roots. Root-associated bacteria are largely influenced these which can be perceived as signals or substrates. Here, we evaluate effect root exudates collected from maize plants grown under nitrogen (N), phosphate (P), iron (Fe) potassium (K) deficiencies on transcriptome plant growth promoting rhizobacterium (PGPR) Bacillus...
Summary To investigate regulatory processes and protective mechanisms leading to desiccation tolerance (DT) in seeds, 16086‐element microarrays were used monitor changes the transcriptome of desiccation‐sensitive 3‐mm‐long radicles Medicago truncatula seeds at different time points during incubation a polyethylene glycol (PEG) solution −1.7 MPa, resulting gradual re‐establishment DT. Gene profiling was also performed on embryos before after acquisition DT maturation. More than 1300 genes...
Plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR exudates, we investigated changes the transcriptome a exudates.Bacillus amyloliquefaciens FZB42 well-studied PGPR. obtain comprehensive overview gene expression response...
Abstract Background Sinorhizobium meliloti is a soil-dwelling α-proteobacterium that possesses large, tripartite genome and engages in nitrogen fixing symbiosis with its plant hosts. Although much known about this important model organism, global characterization of genetic regulatory circuits has been hampered by lack information transcription promoters. Results Using an RNAseq approach RNA populations representing 16 different growth stress conditions, we comprehensively mapped S. start...
Differential RNA-Seq (dRNA-Seq) is a recently developed method of performing primary transcriptome analyses that allows for the genome-wide mapping transcriptional start sites (TSSs) and identification novel transcripts. Although transcriptomes diverse bacterial species have been characterized by dRNA-Seq, analysis archaeal still rather limited. Therefore, we used dRNA-Seq to characterize model archaeon Haloferax volcanii. Three independent cultures Hfx. volcanii grown under optimal...
ABSTRACT In this work, DNA microarrays were used to investigate genome-wide transcriptional responses of Sinorhizobium meliloti a sudden increase in external osmolarity elicited by addition either NaCl or sucrose exponentially growing cultures. A time course the response within first 4 h after osmotic shock was established. We found that there general redundancy differentially expressed genes addition. Both kinds stress resulted induction large number having unknown functions and repression...
Quorum sensing, a population density-dependent mechanism for bacterial communication and gene regulation, plays crucial role in the symbiosis between alfalfa its symbiont Sinorhizobium meliloti. The Sin system, one of three quorum sensing systems present S. meliloti, controls production symbiotically active exopolysaccharide EPS II. Based on DNA microarray data, system also seems to regulate multitude meliloti genes, including genes that participate low-molecular-weight succinoglycan...