A5013221314- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Advanced Proteomics Techniques and Applications
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Scientific Computing and Data Management
- Mass Spectrometry Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Plant Virus Research Studies
- Lipid Membrane Structure and Behavior
- Metal Extraction and Bioleaching
- CRISPR and Genetic Engineering
- Amino Acid Enzymes and Metabolism
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
Max Planck Institute of Biochemistry
2016-2025
Universität Ulm
2024-2025
University of Freiburg
2023
Centre for European Economic Research
2001-2021
Max Planck Society
2006-2015
Max Planck Institute for Plasma Physics
2012
Ghent University
2007
Helmholtz Zentrum München
1999
Center for Environmental Health
1999
National Research Council Canada
1997
25 mM potassium phosphate buffer, pH 7.4, containing 200 KCl; EGTA, ethylene glycol bis(8-aminoethyl ether)-N,N,N'
The distribution of receptors for a neurotransmitter was investigated cytochemically the first time in central nervous system, at synapses established on cells ventral horn rat cervical spinal cord. Three monoclonal antibodies (mAb's) raised against glycine were used. Immunofluorescent staining already showed discontinuous labeling surface neurons, and immunoenzymatic electron microscopy further revealed that antigenic determinants confined to postsynaptic membrane concentrated level...
The glycine receptor of rat spinal cord is an oligomeric membrane glycoprotein molecular mass 250,000 daltons that contains three polypeptides 48,000, 58,000, and 93,000 daltons. Monoclonal antibodies (mAbs) were prepared against the affinity-purified protein by using 125I-labeled preparations for detection positive hybrids. From nine monoclonal obtained, six recognized denatured blotted to nitrocellulose paper. Two these bound more than one polypeptide: mAb GlyR 4a stained 48,000-...
Background Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, readily transformable, and has a relatively stable genome. This, in combination with its biochemical genetic tractability, made Hfx. key model organism, not only for the study of halophilicity, but also archaeal biology general. Methodology/Principal Findings We report here sequencing analysis genome DS2, type strain this species. The contains main 2.848 Mb chromosome, three smaller...
Abstract Archaea play indispensable roles in global biogeochemical cycles, yet many crucial cellular processes, including cell-shape determination, are poorly understood. Haloferax volcanii , a model haloarchaeon, forms rods and disks, depending on growth conditions. Here, we used combination of iterative proteomics, genetics, live-cell imaging to identify mutants that only form or disks. We compared the proteomes with wild-type cells across phases, thereby distinguishing between protein...
The square halophilic archaeon Haloquadratum walsbyi dominates NaCl-saturated and MgCl2 enriched aquatic ecosystems, which imposes a serious desiccation stress, caused by the extremely low water activity. genome sequence was analyzed physiological physical experiments were carried out in order to reveal how H. has specialized into its narrow hostile ecological niche found ways cope with stress. A rich repertoire of proteins involved phosphate metabolism, phototrophic growth extracellular...
The Protein Information Resource (PIR; http://wwwnbrf.georgetown.edu/pir/) supports research on molecular evolution, functional genomics, and computational biology by maintaining a comprehensive, non-redundant, well-organized freely available protein sequence database. Since 1988 the database has been maintained collaboratively PIR-International, an international association of data collection centers cooperating to develop this resource during period explosive growth in new computer...
Summary The halophilic γ‐proteobacterium Halomonas elongata DSM 2581 T thrives at high salinity by synthesizing and accumulating the compatible solute ectoine. Ectoine levels are highly regulated according to external salt but overall picture of its metabolism control is not well understood. Apart from critical role in cell adaptation environments, ectoine can be used as a stabilizer for enzymes protectant skin health care applications thus produced annually on scale tons an industrial...
The MIPS group (Martinsried Institute for Protein Sequences) at the Max-Planck-Institute Biochemistry, Martinsried near Munich, Germany, collects, processes and distributes protein sequence data within framework of tripartite association PIR-International Sequence Database (1,2). contributes nearly 50% input to Database. database is distributed on CD-ROM together with PATCHX, an exhaustive supplement unique, unverified sequences from external sources compiled by MIPS. Through its WWW server...
Natronomonas pharaonis is an extremely haloalkaliphilic archaeon that was isolated from salt-saturated lakes of pH 11. We sequenced its 2.6-Mb GC-rich chromosome and two plasmids (131 23 kb). Genome analysis suggests it adapted to cope with severe ammonia heavy metal deficiencies arise at high values. A degree nutritional self-sufficiency predicted confirmed by growth in a minimal medium containing leucine but no other amino acids or vitamins. Genes for complex III analog the respiratory...
A large-scale purification procedure was developed to isolate the glycine receptor of pig spinal cord by affinity chromatography on aminostrychnine agarose. After an overall about 10 000-fold, preparations contained three major polypeptides Mr 48 000, 58 and 93 000. Photoaffinity labeling with [3H]strychnine showed that binding site is associated 000 and, a much lesser extent, polypeptides. [3H]Strychnine purified exhibited dissociation constant KD 13.8 nM inhibited agonists glycine,...
Differential RNA-Seq (dRNA-Seq) is a recently developed method of performing primary transcriptome analyses that allows for the genome-wide mapping transcriptional start sites (TSSs) and identification novel transcripts. Although transcriptomes diverse bacterial species have been characterized by dRNA-Seq, analysis archaeal still rather limited. Therefore, we used dRNA-Seq to characterize model archaeon Haloferax volcanii. Three independent cultures Hfx. volcanii grown under optimal...
Background Haloquadratum walsbyi commonly dominates the microbial flora of hypersaline waters. Its cells are extremely fragile squares requiring >14%(w/v) salt for growth, properties that should limit its dispersal and promote geographical isolation divergence. To assess this, genome sequences two isolates recovered from sites at near maximum distance on Earth, were compared. Principal Findings Both chromosomes 3.1 MB in size, 84% each sequence was highly similar to other (98.6% identity),...
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system provides adaptive and heritable immunity against foreign genetic elements in most archaea many bacteria. Although this is widespread diverse with subtypes, only a few species have been investigated to elucidate the precise mechanisms for defense of viruses or plasmids. Approximately 90% all sequenced encode CRISPR/Cas systems, but their molecular details so far examined three archaeal...
The Munich Information Center for Protein Sequences (MIPS-GSF), Martinsried near Munich, Germany, develops and maintains genome oriented databases. It is commonplace that the amount of sequence data available increases rapidly, but not capacity qualified manual annotation at Therefore, our strategy aims to cope with stream by comprehensive application analysis tools sequences complete genomes, systematic classification protein active support functional genomics projects. This report...
Characterization of protein N-terminal peptides supports the quality assessment data derived from genomic sequences (e.g., correct assignment start codons) and hints to in vivo modifications such as acetylation removal initiator methionine. The current work represents first large-scale identification prokaryotes, two halophilic euryarchaeota Halobacterium salinarum Natronomonas pharaonis. Two methods were used that specifically allow characterization peptides: combined fractional diagonal...
Cell surfaces are critical for diverse functions across all domains of life, from cell-cell communication and nutrient uptake to cell stability surface attachment. While certain aspects the mechanisms supporting biosynthesis archaeal unique, likely due important differences in compositions between domains, others shared with bacteria or eukaryotes both. Based on recent studies completed a phylogenetically array archaea, wide variety habitats, here we discuss advances characterization...
The subcellular organization of biochemical processes in space and time is still one the most mysterious topics archaeal cell biology. Despite fact that haloarchaea largely rely on covalent lipid anchoring to coat envelope, little known about how cells coordinate de novo synthesis insertion this proteinaceous layer throughout cycle. Here, we report identification two novel contributors ArtA-dependent lipid-mediated protein surface, HvPssA HvPssD. ArtA, HvPssA, HvPssD, as well SLG, showed...
Abstract The identification of 114 integral membrane proteins from Halobacterium salinarum was achieved using liquid chromatography/tandem mass spectrometric (LC/MS/MS) techniques, representing 20% the predicted alpha‐helical transmembrane genome. For this experiment, a preparation with only minor contamination by soluble prepared. From number peripheral were identified classical two dimensional gel electrophoresis (2‐DE) approach, but largely failed very few being identified. By use...