A5081594456- Fungal and yeast genetics research
- Fermentation and Sensory Analysis
- Yeasts and Rust Fungi Studies
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Telomeres, Telomerase, and Senescence
- Chromosomal and Genetic Variations
- Biofuel production and bioconversion
- Horticultural and Viticultural Research
- Microbial Metabolic Engineering and Bioproduction
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Evolution and Genetic Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Wine Industry and Tourism
- Wheat and Barley Genetics and Pathology
- Microbial Community Ecology and Physiology
- Diabetes and associated disorders
University of Leicester
2014-2024
Clemson University
2024
Translational Therapeutics (United States)
2023
Leicester College
2023
University of Nebraska at Omaha
2021
Queen's Medical Centre
2006-2015
University of Nottingham
2006-2015
Genomics (United Kingdom)
2012-2014
Institut thématique Génétique, génomique et bioinformatique
2012
Cardiff University
2010
Abstract The Saccharomyces cerevisiae SGS1 gene is homologous to Escherichia coli RecQ and the human BLM WRN proteins that are defective in cancer-prone disorder Bloom's syndrome premature aging Werner's syndrome, respectively. While recQ mutants deficient conjugational recombination DNA repair, cell lines show hyperrecombination. both exhibit chromosomal instability. sgslΔ strains mitotic hyperrecombination, as do cells. This was manifested an increase frequency of interchromosomal...
A fundamental goal in biology is to achieve a mechanistic understanding of how and what extent ecological variation imposes selection for distinct traits favors the fixation specific genetic variants. Key such an detailed mapping natural genomic phenomic space bridging gap that separates these worlds. Here we chart high-resolution map trait one most important model organisms, budding yeast Saccharomyces cerevisiae, its closest wild relatives trace basis timing major phenotype changing events...
Algorithms for generating the exact distribution of a finite sample drawn from population in Hardy-Weinberg equilibrium are given multiple alleles. The sampling is derived analogously to Fisher's 2 X and equivalent Levene's conditional populations. algorithms presented fast computationally allow quick alternatives standard methods requiring corrections approximations. Computation time on order few seconds three-allele examples up minutes four-allele an IBM 3081 machine.
Subtelomeric regions are often under-represented in genome sequences of eukaryotes. One the best known examples use telomere proximity for adaptive purposes bloodstream expression sites (BESs) African trypanosome Trypanosoma brucei. To enhance our understanding BES structure and function host adaptation immune evasion, repertoire from Lister 427 strain T. brucei were independently tagged sequenced. BESs polymorphic size but reveal a surprisingly conserved architecture context extensive...
The question of how genetic variation in a population influences phenotypic and evolution is major importance modern biology.Yet much still unknown about the relative functional different forms genome they are shaped by evolutionary processes.Here we address these questions level sequencing 42 strains from budding yeast Saccharomyces cerevisiae its closest S. paradoxus.We find that content variation, form presence or absence as well copy number material, higher within than paradoxus, despite...
One approach to understanding the genetic basis of traits is study their pattern inheritance among offspring phenotypically different parents. Previously, such analysis has been limited by low mapping resolution, high labor costs, and large sample size requirements for detecting modest effects. Here, we present a novel map trait loci using artificial selection. First, generated populations 10–100 million haploid diploid segregants crossing two budding yeast strains heat tolerance up 12...
Using the biological species definition, yeasts of genus Saccharomyces sensu stricto comprise six and one natural hybrid. Previous work has shown that reproductive isolation between is due primarily to sequence divergence acted upon by mismatch repair system not major gene differences or chromosomal rearrangements. Sequence through also been cause partial among populations within a species. We have surveyed variation in measured meiotic sterility hybrids. This allows us determine necessary...
On the basis of genetic analysis, molecular karyotyping and sequence analyses 18S rRNA internal transcribed spacer (ITS) region, three new Saccharomyces species are described, cariocanus (with type strain NCYC 2890T), kudriavzevii 2889T) mikatae 2888T). Genetic did not confirm previously observed conspecificity paradoxus S. cariocanus. The latter exhibits postzygotic isolation from representative strains all known geographical populations paradoxus: European, Far-East Asian, North American Hawaiian.
The complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII reveals that it contains 269 predicted or known genes (300 base pairs larger). Fifty-nine these (22 percent) were previously identified. Of the 210 novel genes, 65 are to encode proteins similar other function. Sixteen appear be relatively recently duplicated. On average, there is one gene approximately every 2 kilobases. Although coding density and composition across not uniform, no regular pattern variation apparent.
Abstract Yeast chromosome ends are composed of several different repeated elements. Among six clones from two strains Saccharomyces cerevisiae, at least seven sequence families were found. These included the previously identified Y' and X Some highly variable in copy number location between S. while other elements appear constant location. Three specific to cerevisiae not found its evolutionarily close relative, paradoxus. Two sequences both None those described here is (by low stringency...
Abstract Phenotypic variation arising from populations adapting to different niches has a complex underlying genetic architecture. A major challenge in modern biology is identify the causative variants driving phenotypic variation. Recently, baker’s yeast, Saccharomyces cerevisiae emerged as powerful model for dissecting traits. However, past studies using laboratory strain were unable reveal complete architecture of polygenic Here, we present linkage study 576 recombinant strains obtained...