A5082682293- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Plant-Microbe Interactions and Immunity
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Enzyme Production and Characterization
- Microbial Natural Products and Biosynthesis
- Plant Disease Resistance and Genetics
- Wastewater Treatment and Nitrogen Removal
- Agronomic Practices and Intercropping Systems
- CRISPR and Genetic Engineering
- Coastal wetland ecosystem dynamics
- Polysaccharides and Plant Cell Walls
- Viral Infectious Diseases and Gene Expression in Insects
- Diphtheria, Corynebacterium, and Tetanus
- Microbial metabolism and enzyme function
- Probiotics and Fermented Foods
Bielefeld University
2015-2024
Heinrich Heine University Düsseldorf
2021
Institut für biologische Forschung
2004-2014
Center for Systems Biology Dresden
2012
Estación Experimental del Zaidín
2007
Universidad Nacional de La Plata
1992-2007
Universidad de Sevilla
2007
Consejo Superior de Investigaciones Científicas
2007
Instituto de Biotecnología y Biología Molecular
2007
Elisabeth-Krankenhaus Essen
2007
The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain compounds by bacterial reduction dinitrogen (N 2 ) ammonium (NH 4 + ). We present here the annotated DNA sequence α-proteobacterium Sinorhizobium meliloti , symbiont alfalfa. tripartite 6.7-megabase (Mb) genome comprises a 3.65-Mb chromosome, and 1.35-Mb pSymA 1.68-Mb pSymB megaplasmids. Genome analysis indicates that all three elements contribute, in...
To detect plasmid-borne antibiotic-resistance genes in wastewater treatment plant (WWTP) bacteria, 192 resistance-gene-specific PCR primer pairs were designed and synthesized. Subsequent analyses on total plasmid DNA preparations obtained from bacteria of activated sludge or the WWTP's final effluents led to identification of, respectively, 140 123 different amplicons. The detected included aminoglycoside, beta-lactam, chloramphenicol, fluoroquinolone, macrolide, rifampicin, tetracycline,...
Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. becomes the dominant microbe oil-polluted waters. SK2 has streamlined genome with paucity mobile genetic elements energy generation–related genes, but plethora genes accounting for wide hydrocarbon substrate range efficient oil-degradation capabilities. The further specifies systems scavenging nutrients,...
The genus Sorangium synthesizes approximately half of the secondary metabolites isolated from myxobacteria, including anti-cancer metabolite epothilone. We report complete genome sequence model strain S. cellulosum So ce56, which produces several natural products and has morphological physiological properties typical genus. circular genome, comprising 13,033,779 base pairs, is largest bacterial sequenced to date. No global synteny with Myxococcus xanthus apparent, revealing an unanticipated...
The introduction of next generation sequencing approaches has caused a rapid increase in the number completely sequenced genomes. As one result this development, it is now feasible to analyze large groups related genomes comparative approach. A main task genomics identification orthologous genes different and classification as core or singletons.To support these studies EDGAR - "Efficient Database framework for Genome Analyses using BLAST score Ratios" was developed. designed automatically...
The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying infection strategies. Here, we present whole-genome sequence pepper-pathogenic strain 85-10, comprises 5.17-Mb circular chromosome four plasmids. genome high G+C content (64.75%) signatures extensive plasticity....
The whole-genome-sequenced rhizobacterium Bacillus amyloliquefaciens FZB42(T) (Chen et al., 2007) and other plant-associated strains of the genus described as belonging to species or subtilis are used commercially promote growth improve health crop plants. Previous investigations revealed that a group represented distinct ecotype related B. amyloliquefaciens; however, exact taxonomic position this remains elusive (Reva 2004). In present study, we demonstrated ability closely strain colonize...
Cyanobacteria forged two major evolutionary transitions with the invention of oxygenic photosynthesis and bestowal photosynthetic lifestyle upon eukaryotes through endosymbiosis. Information germane to understanding those is imprinted in cyanobacterial genomes, but deciphering it complicated by lateral gene transfer (LGT). Here, we report genome sequences for morphologically most complex true-branching cyanobacteria, Scytonema hofmanni PCC 7110, which 12,356 proteins gene-rich prokaryote...
Biogas production from renewable resources is attracting increased attention as an alternative energy source due to the limited availability of traditional fossil fuels. Many countries are promoting use sources for sustainable production. In this study, a metagenome production-scale biogas fermenter was analysed employing Roche's GS FLX Titanium technology and compared previous dataset obtained same community DNA sample that sequenced on platform. Taxonomic profiling based 16S rRNA-specific...
Sinorhizobium meliloti is an α-proteobacterium that forms agronomically important N 2 -fixing root nodules in legumes. We report here the complete sequence of largest constituent its genome, a 62.7% GC-rich 3,654,135-bp circular chromosome. Annotation allowed assignment function to 59% 3,341 predicted protein-coding ORFs, rest exhibiting partial, weak, or no similarity with any known sequence. Unexpectedly, level reiteration within this replicon low, only two genes duplicated more than 90%...
Abstract Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting various stress conditions. In this study, we focused on the overlapping genetic program activated by two commonly studied in addition to identifying AM-related genes. We thus applied 16,086 probe microarrays profile transcriptome of model legume Medicago truncatula during interactions with Glomus mosseae intraradices...
Analysis of the 1,683,333-nt sequence pSymB megaplasmid from symbiotic N 2 -fixing bacterium Sinorhizobium meliloti revealed that replicon has a high gene density with total 1,570 protein-coding regions, few insertion elements and regions duplicated elsewhere in genome. The only copies an essential arg-tRNA minCDE genes are located on pSymB. Almost 20% carries encoding solute uptake systems, most which were ATP-binding cassette family. Many previously unsuspected involved polysaccharide...
Abstract In this study, we describe a large-scale expression-profiling approach to identify genes differentially regulated during the symbiotic interaction between model legume Medicago truncatula and nitrogen-fixing bacterium Sinorhizobium meliloti. Macro- microarrays containing about 6,000 probes were generated on basis of three cDNA libraries dedicated study root interactions. The experiments performed wild-type mutant material led us set 756 either up- or down-regulated at different...
Azoarcus sp. strain BH72, a mutualistic endophyte of rice and other grasses, is agrobiotechnological interest because it supplies biologically fixed nitrogen to its host colonizes plants in remarkably high numbers without eliciting disease symptoms. The complete genome sequence 4,376,040-bp long contains 3,992 predicted protein-coding sequences. Genome comparison with the Azoarcus-related soil bacterium EbN1 revealed surprisingly low degree synteny. Coding sequences involved synthesis...