A5059189176- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Microbial bioremediation and biosurfactants
- Enzyme Production and Characterization
- Gut microbiota and health
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Methane Hydrates and Related Phenomena
- Metabolomics and Mass Spectrometry Studies
- TiO2 Photocatalysis and Solar Cells
- Advanced Proteomics Techniques and Applications
- Diet and metabolism studies
- Advanced Photocatalysis Techniques
- Protein Structure and Dynamics
- Metal Extraction and Bioleaching
- Nanoparticles: synthesis and applications
- Clostridium difficile and Clostridium perfringens research
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- biodegradable polymer synthesis and properties
- Analytical Chemistry and Chromatography
- Carbohydrate Chemistry and Synthesis
- Microplastics and Plastic Pollution
Instituto de Catálisis y Petroleoquímica
2016-2025
Consejo Superior de Investigaciones Científicas
2016-2025
Unidades Centrales Científico-Técnicas
2022-2025
Universidade Estadual de Maringá
2023
Institute of Catalysis and Petrochemistry
2013-2022
Marie Curie
2008-2022
Universidad San Pablo CEU
2015-2018
Universidad de Oviedo
2015
Helmholtz Centre for Environmental Research
2015
Universidad de Navarra
2015
<h3>Objective</h3> Antibiotic (AB) usage strongly affects microbial intestinal metabolism and thereby impacts human health. Understanding this process the underlying mechanisms remains a major research goal. Accordingly, we conducted first comparative omic investigation of gut communities in faecal samples taken at multiple time points from an individual subjected to β-lactam therapy. <h3>Methods</h3> The total (16S rDNA) active rRNA) microbiota, metagenome, metatranscriptome (mRNAs),...
Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. becomes the dominant microbe oil-polluted waters. SK2 has streamlined genome with paucity mobile genetic elements energy generation–related genes, but plethora genes accounting for wide hydrocarbon substrate range efficient oil-degradation capabilities. The further specifies systems scavenging nutrients,...
Titania (TiO₂)-based nanocomposites subjected to light excitation are remarkably effective in eliciting microbial death. However, the mechanism by which these materials induce death and effects that they have on microbes poorly understood. Here, we assess low dose radical-mediated TiO₂ photocatalytic action of such evaluate genome/proteome-wide expression profiles Pseudomonas aeruginosa PAO1 cells after two minutes intervention. The results indicate impact gene-wide flux distribution...
Biodegradation of synthetic polymers, in particular polyethylene terephthalate (PET), is great importance, since environmental pollution with PET and other plastics has become a severe global problem. Here, we report on the polyester degrading ability novel carboxylic ester hydrolase identified genome marine hydrocarbonoclastic bacterium Pseudomonas aestusnigri VGXO14 T . The enzyme, designated PE-H, belongs to type IIa family hydrolytic enzymes as indicated by amino acid sequence homology....
Summary Recent research has disclosed a tight connection between obesity, metabolic gut microbial activities and host health. Obtaining complete understanding of this relationship remains major goal. Here, we conducted comparative metagenomic metaproteomic investigation communities in faecal samples taken from an obese lean adolescent. By analysing the diversity 16S rDNA amplicons (10% operational phylogenetic units being common), 22 Mbp consensus metagenome sequences (∼ 70% common)...
A metagenome expression library of bulk DNA extracted from the rumen content a dairy cow was established in phage lambda vector and activity-based screening employed to explore functional diversity microbial flora. Twenty-two clones specifying distinct hydrolytic activities (12 esterases, nine endo-beta-1,4-glucanases one cyclodextrinase) were identified characterized. Sequence analysis retrieved enzymes revealed that eight (36%) entirely new formed deep-branched phylogenetic lineages with...
RL5, a gene coding for novel polyphenol oxidase, was identified through activity screening of metagenome expression library from bovine rumen microflora. Characterization the recombinant protein produced in Escherichia coli revealed multipotent capacity to oxidize wide range substrates (syringaldazine > 2,6-dimethoxyphenol veratryl alcohol guaiacol tetramethylbenzidine 4-methoxybenzyl 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) >> phenol red) over an unusually broad pH 3.5...
Experimental data exists for only a vanishingly small fraction of sequenced microbial genes. This community page discusses the progress made by COMBREX project to address this important issue using both computational and experimental resources.