A5089096772- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Electrocatalysts for Energy Conversion
- Molecular Junctions and Nanostructures
- Metalloenzymes and iron-sulfur proteins
- Conducting polymers and applications
- Microbial Fuel Cells and Bioremediation
- Enzyme-mediated dye degradation
- Enzyme Catalysis and Immobilization
- Fuel Cells and Related Materials
- Photoreceptor and optogenetics research
- Microbial Metabolic Engineering and Bioproduction
- Advanced Nanomaterials in Catalysis
- Neuroscience and Neural Engineering
- CO2 Reduction Techniques and Catalysts
- Polymer Surface Interaction Studies
- Advanced Photocatalysis Techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Advanced battery technologies research
- Ammonia Synthesis and Nitrogen Reduction
- Nanocluster Synthesis and Applications
- ATP Synthase and ATPases Research
Instituto de Catálisis y Petroleoquímica
2016-2025
Institute of Catalysis and Petrochemistry
2022-2023
Universidad Autónoma de Madrid
2006-2020
Consejo Superior de Investigaciones Científicas
2006-2019
Robert Bosch (Germany)
2016
Clarkson University
2007-2016
Applus+ IDIADA (Spain)
2013
Dartmouth College
2011
State University of New York at Potsdam
2011
Materials Processing (United States)
2011
Abstract Nitrogenases are the only enzymes known to reduce molecular nitrogen (N 2 ) ammonia (NH 3 ). By using methyl viologen ( N , ′‐dimethyl‐4,4′‐bipyridinium) shuttle electrons nitrogenase, reduction NH can be mediated at an electrode surface. The coupling of this nitrogenase cathode with a bioanode that utilizes enzyme hydrogenase oxidize hydrogen (H results in enzymatic fuel cell (EFC) is able produce from H and while simultaneously producing electrical current. To demonstrate this,...
This paper presents an efficient strategy for the specific immobilization of fully functional proteins onto surface nanoparticles. Thioctic acid-derivatized gold clusters are used as a scaffold further stepwise modification, leading to cobalt(II)-terminated ligand shell. A histidine tag introduced by genetic engineering into protein is coordinated this transition metal ion. The has been demonstrated cases genetically engineered horseradish peroxidase and ferredoxin-NADP+ reductase,...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTBiocomputing Security System: Concatenated Enzyme-Based Logic Gates Operating as a Biomolecular Keypad LockGuinevere Strack, Maryna Ornatska, Marcos Pita, and Evgeny KatzView Author Information Department of Chemistry Science, Clarkson University, Potsdam, New York 13699-5810 Cite this: J. Am. Chem. Soc. 2008, 130, 13, 4234–4235Publication Date (Web):March 6, 2008Publication History Received29 December 2007Published online6 March...
Direct electron transfer (DET) reactions between redox enzymes and electrodes can be maximized by oriented immobilization of the enzyme molecules onto an electroactive surface modified with functionalized gold nanoparticles (AuNPs). Here, we present such strategy for obtaining a DET-based laccase (Lc) cathode O(2) electroreduction at low overpotentials. The stable nanostructured enzymatic electrode is based on step-by-step covalent attachment AuNPs Lc to porous graphite using diazonium salt...
RL5, a gene coding for novel polyphenol oxidase, was identified through activity screening of metagenome expression library from bovine rumen microflora. Characterization the recombinant protein produced in Escherichia coli revealed multipotent capacity to oxidize wide range substrates (syringaldazine > 2,6-dimethoxyphenol veratryl alcohol guaiacol tetramethylbenzidine 4-methoxybenzyl 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) >> phenol red) over an unusually broad pH 3.5...
A new signal-responsive interface with switchable/tunable redox properties based on a pH-responding polymer brush was studied. Poly(4-vinyl pyridine), P4VP, functionalized Os-complex units grafted to an indium tin oxide (ITO) conductive support in the form of brush. The modified electrode surface responsive changes pH value electrolyte solution: at acidic = 4.0 redox-polymer film demonstrated reversible electrochemical process, E° 0.29 V (vs Ag/AgCl), while neutral > 6, not electrochemically...
An enzyme-based biofuel cell with a pH-switchable oxygen electrode, controlled by enzyme logic operations processing in situ biochemical input signals, has been developed. Two Boolean gates (AND/OR) were assembled from systems to process signals and convert them logically into pH-changes of the solution. The cathode used was modified polymer-brush functionalized Os-complex redox species operating as relay units mediate electron transport between conductive support soluble laccase...
The logic network composed of three enzymes (alcohol dehydrogenase, glucose and oxidase) operating in concert as four concatenated gates (AND/OR), was designed to process different chemical input signals (NADH, acetaldehyde, glucose, oxygen). cascade biochemical reactions culminated pH changes controlled by the pattern applied signals. "successful" set inputs produced gluconic acid final product yielded an acidic medium, lowering a solution from its initial value 6−7 ca. 4. whole signal...
Here for the first time, we detail self-contained (wireless and self-powered) biodevices with wireless signal transmission. Specifically, demonstrate operation of self-sustained carbohydrate oxygen sensitive biodevices, consisting a electronic unit, radio transmitter separate sensing bioelectrodes, supplied electrical energy from combined multi-enzyme fuel cell generating sufficient current at required voltage to power electronics. A carbohydrate/oxygen enzymatic was assembled by comparing...
After uptake by U87 MG and A375 cancer cells, cobaltabisdicarbollide [COSAN]- distributes between membrane nucleus presents no relevant cytotoxicity against both cell lines even for long incubation times. The of Na[COSAN] was also tested towards one normal line, the V79 fibroblasts, in order to ascertain noncytotoxic profile compound. As cell's contains DNA, interaction double-stranded calf thymus DNA (CT-dsDNA) has been investigated. There is a strong molecules forming nanohybrid...
The pH-responsive nanoparticles were coupled with information-processing enzyme-based systems to yield "smart" signal-responsive hybrid built-in Boolean logic. enzyme performed AND/OR logic operations, transducing biochemical input signals into reversible structural changes (signal-directed self-assembly) of the nanoparticle assemblies, thus resulting in processing and amplification signals. system mimics biological effective complex information, self-assembled structures nanoparticles....
We develop an approach aimed at optimizing the parameters of a network biochemical logic gates for reduction "analog" noise buildup. Experiments three coupled enzymatic AND are reported, illustrating our procedure. Specifically, starch, one controlled inputs, is converted to maltose by beta-amylase. With use phosphate (another input), phosphorylase then produces glucose. Finally, nicotinamide adenine dinucleotide (NAD(+)), third input, reduced under action glucose dehydrogenase yield...
Biochemical systems that demonstrate the Boolean logic operations AND, OR, XOR, and InhibA were developed by using soluble compounds, which represent chemical "devices", enzymes glucose oxidase (GOx), dehydrogenase (GDH), alcohol (AlcDH), microperoxidase-11 (MP-11), operated as input signals activated gates. The used materials immobilized biocatalysts. studied are proposed to be a step towards construction of "smart" signal-responsive with built-in logic.
A pH-responsive mixed polyelectrolyte brush from tethered polyacrylic acid (PAA) and poly(2-vinylpyridine) (P2VP) (PAA:P2VP = 69:31 by weight) was prepared used for selective gating transport of anions cations across the thin film. An ITO glass electrode modified with polymer to study switchable permeability triggered changes in pH aqueous environment presence two soluble redox probes: [Fe(CN)(6)](4-) [Ru(NH(3))(6)](3+). The responsive behavior also investigated using situ ellipsometric...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTBiochemically Controlled Bioelectrocatalytic InterfaceTsz Kin Tam, Jian Zhou, Marcos Pita, Maryna Ornatska, Sergiy Minko, and Evgeny KatzView Author Information Department of Chemistry Biomolecular Science NanoBio Laboratory (NABLAB), Clarkson University, Potsdam, New York 13699-5810[email protected]; [email protected]Cite this: J. Am. Chem. Soc. 2008, 130, 33, 10888–10889Publication Date (Web):July 24, 2008Publication History Received10 June...
Biofuel cells with a switchable power release controlled by biochemical signals, which can be logically processed biomolecular computing systems, have been designed. The properties of the biofuel were based on polymer-brush-modified electrodes activity dependent solution pH value. changes generated in situ biocatalytic reactions allowed reversible activation-inactivation bioelectrocatalytic interfaces-thus affecting entire cells. Boolean logic operations performed either enzyme- or...
The logic gates NAND/NOR were mimicked by enzyme biocatalyzed reactions activated sucrose, maltose and phosphate. subunits performing AND/OR Boolean operations designed using phosphorylase cooperative work of invertase/amyloglucosidase, respectively. Glucose produced as the output signal from was applied input for INVERTER gate composed alcohol dehydrogenase, glucose oxidase, microperoxidase-11, ethanol NAD+, which generated final in form NADH inverting 0 to 1 or 0. amplified a...
A new strategy for oriented covalent immobilization of Trametes hirsuta laccase on gold electrodes is presented. The based the surface modification with a mixed monolayer an aromatic diazonium salt derivative and 6-mercapto-1-hexanol further use as scaffold enzyme's linkage. This offers variety advantages such high stability laccase-friendly support morphology, which turns it into suitable metal–enzyme interface. Conditions aiming at optimum orientation direct electron transfer (DET) via T1...