A5006267287- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Fungal and yeast genetics research
- Enzyme Structure and Function
- Carbohydrate Chemistry and Synthesis
- Enzyme-mediated dye degradation
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- Digestive system and related health
- Microbial Metabolism and Applications
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Plant tissue culture and regeneration
- Probiotics and Fermented Foods
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Plant nutrient uptake and metabolism
- Fermentation and Sensory Analysis
- Advanced Cellulose Research Studies
Instituto de Agroquímica y Tecnología de Alimentos
2016-2025
Consejo Superior de Investigaciones Científicas
2013-2024
Unidades Centrales Científico-Técnicas
2013
Universidad Autónoma Metropolitana
2005
Instituto de Química Física Blas Cabrera
1994
Centro de Biología Molecular Severo Ochoa
1986
Universidad Autónoma de Madrid
1986
Carlsberg Laboratory
1984
National Renewable Energy Laboratory
1983
Universidad de Sevilla
1981-1982
Carbohydrate Active Enzymes -- Amylolytic Enzymes: Types, Structures and Specificities The Use of Starch Processing in the Food Industry Cellulases For Biomass Conversion Textile Xylanases: Molecular Properties Applications Microbial Xylanolytic Esterases Structural Biochemical Pectinases ?-L-rhamnosidases: Old New Insights Application Glycosidases Transglycosidases Synthesis Oligosaccharides Peptidases An Introduction to Merops Database Cysteine Proteases Subtilisin Aspartic Used Cheese...
RL5, a gene coding for novel polyphenol oxidase, was identified through activity screening of metagenome expression library from bovine rumen microflora. Characterization the recombinant protein produced in Escherichia coli revealed multipotent capacity to oxidize wide range substrates (syringaldazine > 2,6-dimethoxyphenol veratryl alcohol guaiacol tetramethylbenzidine 4-methoxybenzyl 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) >> phenol red) over an unusually broad pH 3.5...
A complete saccharification of plant polymers is the critical step in efficient production bio-alcohols. Beta-glucosidases acting degradation intermediate gluco-oligosaccharides produced by cellulases limit yield final product.
Microbial communities from cow rumen are known for their ability to degrade diverse plant polymers at high rates. In this work, we identified 15 hydrolases through an activity-centred metagenome analysis of a fibre-adherent microbial community dairy rumen. Among them, 7 glycosyl (GHs) and 1 feruloyl esterase were successfully cloned, expressed, purified characterised. The most striking result was protein GH family 43 (GHF43), hereinafter designated as R_09-02, which had characteristics very...
The guts and casts of earthworms contain microbial assemblages that process large amounts organic polymeric substrates from plant litter soil; however, the enzymatic potential these communities remains largely unexplored. In present work, we retrieved carbohydrate-modifying enzymes through activity screening metagenomic fosmid libraries cellulose-depleting established with fresh two earthworm species, Aporrectodea caliginosa Lumbricus terrestris, as inocula. Eight glycosyl hydrolases (GHs)...
Efficient enzymatic synthesis of tailor-made prebiotic fructo-oligosaccharides (FOS) used in functional food formulation is a relevant biotechnological objective. We have engineered the Saccharomyces cerevisiae invertase (Suc2) to improve its transferase activity and identify determinants for product specificity. Amino acid replacement (W19Y, N21S, N24S) within conserved motif (β-fructosidase) specifically increased 6-kestose up 10-fold. Mutants with lower substrate (sucrose) affinity...
The β-glucosidase encoded by the<i>bglA</i> gene from <i>Paenibacillus polymyxa</i> has a half-life time of 15 min at 35 °C and no detectable activity 55 °C. We have isolated random mutations that enhance the thermoresistance enzyme. Following directed evolution strategy, we combined some to obtain with 12 65 °C, in range resistance thermophilic enzymes. No significant alteration kinetic parameters enzyme was observed. One mutants screening for thermoresistant had same denaturation as wild...
The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, activities. Here, we present comprehensive biochemical analyses of seven distinct metagenome Lake Arreo, evaporite karstic lake in Spain (42°46'N, 2°59'W; altitude, 655 m). Together with oligonucleotide usage patterns BLASTP analysis, our study esterases/lipases mined Arreo suggests that its sediment contains moderately halophilic cold-adapted proteobacteria containing DNA...
Worldwide, there are various feedstocks such as straws, corn stover, sugarcane bagasse, sweet sorghum bagasse (SSB), grasses, leaves, whey permeate, household organic waste, and food waste (FW) that can be converted to valuable biofuels butanol. For the present studies, an economic analysis was performed compare butanol production from three (SSB; FW; yellow top presscake, YTP or YT) using a standard process advanced integrated design. The total plant capacity set at 170,000–171,000 metric...
Grx5 defines a family of yeast monothiol glutaredoxins that also includes Grx3 and Grx4. All three proteins display significant sequence homology with found from bacteria to humans. is involved in iron/sulfur cluster assembly at the mitochondria, but function Grx4 unknown. Three-dimensional modeling based on known dithiol glutaredoxin structures predicted thioredoxin fold structure for Grx5. Positionally conserved amino acids this were replaced Grx5, effect biological protein has been...
Paenibacillus barcinonensis is a soil bacterium bearing complex set of enzymes for xylan degradation, including several secreted and Xyn10B, one the few intracellular xylanases reported to date. The crystal structure Xyn10B has been determined by x-ray analysis. enzyme folds into typical (β/α)8 barrel family 10 glycosyl hydrolases (GH10), with additional secondary elements within β/α motifs. One these loops -L7- located at β7 C terminus, was essential xylanase activity as its partial...