A5048476222- Entomopathogenic Microorganisms in Pest Control
- Microbial Natural Products and Biosynthesis
- Insect symbiosis and bacterial influences
- Nematode management and characterization studies
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Insect Resistance and Genetics
- Chemical Synthesis and Analysis
- Mosquito-borne diseases and control
- Insect Pest Control Strategies
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Microbial Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Insect and Arachnid Ecology and Behavior
- Enzyme Catalysis and Immobilization
- Advanced Proteomics Techniques and Applications
- Plant-Microbe Interactions and Immunity
- Protist diversity and phylogeny
- Studies on Chitinases and Chitosanases
- Enzyme function and inhibition
- Marine Sponges and Natural Products
- Inorganic Fluorides and Related Compounds
- Machine Learning in Bioinformatics
Max Planck Institute for Terrestrial Microbiology
2022-2024
Adnan Menderes University
2023
Bridge University
2023
Goethe University Frankfurt
2011-2021
Merck (Germany)
2014
Saarland University
2007-2009
The genus Sorangium synthesizes approximately half of the secondary metabolites isolated from myxobacteria, including anti-cancer metabolite epothilone. We report complete genome sequence model strain S. cellulosum So ce56, which produces several natural products and has morphological physiological properties typical genus. circular genome, comprising 13,033,779 base pairs, is largest bacterial sequenced to date. No global synteny with Myxococcus xanthus apparent, revealing an unanticipated...
Members of the genus Xenorhabdus are entomopathogenic bacteria that associate with nematodes. The nematode-bacteria pair infects and kills insects, both partners contributing to insect pathogenesis providing nutrition nematode from available insect-derived nutrients. provides protection predators, access nutrients, a mechanism dispersal. bacterial Photorhabdus also nematodes kill genera provide similar services their different hosts through unique physiological metabolic mechanisms. We...
Abstract Exchange of the native promoter to arabinose‐inducible P BAD was established in entomopathogenic bacteria silence and/or activate gene clusters involved natural product biosynthesis. This allowed “on‐demand” production GameXPeptides, xenoamicins, and blue pigment indigoidine. The for novel “mevalagmapeptides” highly toxic xenorhabdins were identified by this approach.
Natural products (NPs) from microorganisms have been important sources for discovering new therapeutic and chemical entities. While their corresponding biosynthetic gene clusters (BGCs) can be easily identified by gene-sequence-similarity-based bioinformatics strategies, the actual access to these NPs structure elucidation bioactivity testing remains difficult. Deletion of encoding RNA chaperone, Hfq, results in strains losing production most NPs. By exchanging native promoter a desired BGC...
Non-Ribosomal Peptides (NRPs) represent a biomedically important class of natural products that include multitude antibiotics and other clinically used drugs. NRPs are not directly encoded in the genome but instead produced by metabolic pathways biosynthetic gene clusters (BGCs). Since existing mining tools predict many putative synthesized given BGC, it remains unclear which these correct how to identify post-assembly modifications amino acids blind mode, without knowing exist sample. To...
Parasitic nematodes infect different species of animals and plants. Root-knot are members the genus Meloidogyne, which is distributed worldwide parasitizes numerous plants, including vegetables, fruits, crops. To reduce global burden nematode infections, only a few chemical therapeutic classes currently available. The majority nematicides prohibited due to their harmful effects on environment public health. This study was intended identify new nematicidal natural products (NPs) from...
Janthinobacteria commonly form biofilms on eukaryotic hosts and are known to synthesize antibacterial antifungal compounds. Janthinobacterium sp. HH01 was recently isolated from an aquatic environment its genome sequence established. The consists of a single chromosome reveals size 7.10 Mb, being the largest janthinobacterial so far known. Approximately 80% 5,980 coding sequences (CDSs) present in could be assigned putative functions. encodes wealth secretory functions several large clusters...
Abstract Natural products have been proven to be important starting points for the development of new drugs. Bacteria in genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites compete with other organisms. Our study is first comprehensive screening anti-protozoal activity supernatants containing produced by 5 22 species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica Trypanosoma...
Abstract An E. coli strain with deletions in five transaminases (Δ aspC Δ ilvE tyrB avtA ybfQ ) was constructed to be unable degrade several amino acids. This used as an expression host for the analysis of acid configuration nonribosomally synthesized peptides, including novel peptide “xenotetrapeptide” from Xenorhabdus nematophila , by using a combination labeling experiments and mass spectrometry. Additionally, number D ‐amino acids produced assigned following simple cultivation 2 O.
Abstract Our study aimed to identify the novel acaricidal compound in Xenorhabdus szentirmaii and X. nematophila using easyPACId approach (easy Promoter Activated Compound Identification). We determined (1) effects of cell-free supernatant (CFS) obtained from mutant strains against T. urticae females , (2) CFS bioactive strain (pCEP_kan_XNC1_1711) different biological stages urticae, predatory mites, Phytoseiulus persimilis Neoseiulus californicus (3) extracted on (4) cytotoxicity active...
Abstract Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes known to produce array natural products with various activities including larvicidal activity. In this study, the effects produced four one bacteria ovipositional...
Cell differentiation is widespread during the development of multicellular organisms, but rarely observed in prokaryotes. One example prokaryotic gram-negative bacterium Myxococcus xanthus. In response to starvation, this gliding initiates a complex developmental programme that results formation spore-filled fruiting bodies. How cells metabolically support necessary cellular from rod-shaped vegetative into spherical spores unknown. Here, we present evidence intracellular lipid bodies provide...
Two new and five known oxazoles were identified from two different Pseudomonas strains in addition to the pyrones pseudopyronine A B. Labeling experiments confirmed their structures gave initial evidence for a novel biosynthesis pathway of these natural oxazoles. In order confirm structure, they synthesized, which also allowed tests bioactivity. Additionally, bioactivities synthesis intermediates investigated revealing interesting biological activities several compounds despite overall...
Summary Xenorhabdus doucetiae , the bacterial symbiont of entomopathogenic nematode Steinernema diaprepesi produces several different fatty acid amides. Their biosynthesis has been studied using a combination analysis gene deletions and promoter exchanges in X. heterologous expression candidate genes E. coli . While decarboxylase is required for formation all observed phenylethylamides tryptamides, acyltransferase XrdE encoded xenorhabdin cluster responsible short chain acyl Additionally,...
The easyPACId (easy Promoter Activation and Compound Identification) approach is focused on the targeted activation of natural product biosynthetic gene clusters (BGCs) encoding non-ribosomal peptide synthetases (NRPS), polyketide synthases (PKS), NRPS-PKS hybrids, or other BGC classes. It was applied to entomopathogenic bacteria genera Xenorhabdus Photorhabdus by exchanging promoter desired BGCs against L-arabinose inducible PBAD in ∆hfq mutants respective strains. crude (culture) extracts...
Abstract Natural products (NPs) from microorganisms have been important sources for discovering new therapeutic and chemical entities. While their corresponding biosynthetic gene clusters (BGCs) can be easily identified by gene‐sequence‐similarity‐based bioinformatics strategies, the actual access to these NPs structure elucidation bioactivity testing remains difficult. Deletion of encoding RNA chaperone, Hfq, results in strains losing production most NPs. By exchanging native promoter a...
Abstract Natural products have been proven to be important starting points for the development of new drugs. Bacteria in genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites compete with other organisms. Our study is first comprehensive screening anti-protozoal activity supernatants containing produced by 5 22 species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica Trypanosoma...
Abstract Viral diseases like yellow fever, dengue, zika have an alarming impact on public health affecting millions of people around the globe. These can be transmitted by Aedes albopictus – a species originally endemic to South Asia but now widely distributed in several countries outside Asia. Xenorhabdus and Photorhabdus spp., are enteric Gram-negative bacterial symbionts insect-preying nematodes known produce array natural products (NP) with various activities including larvicidal...
The fatty acid (FA) profiles of myxobacteria contain FA species with double bonds at the Delta(5) and Delta(11) positions, latter being rather unusual among bacteria. Despite this knowledge, mechanism for introduction these has never been described before in myxobacteria. Searches candidate genes genome model organism Myxococcus xanthus revealed 16 genes, which have annotated as desaturases. However, due to redundant substrate specificity, functional analyses enzymes by construction...
Xenorhabdins (XRDs) are produced by Xenorhabdus species and members of the dithiopyrrolone (DTP) class natural products that have potent antibacterial, antifungal anticancer activity. The amide moiety their DTP core can be methylated or not to fine-tune bioactivity properties. However, enzyme responsible for N-methylation remained elusive. Here, we identified characterized methyltransferase XrdM is encoded nearly 600 kb away from XRD gene cluster using proteomic analysis, candidate...
Abstract Dithiolopyrrolone (DTP) natural products are produced by several different bacteria and have potent antibacterial, antifungal anticancer activities. While the amide of their DTP core can be methylated to fine‐tune bioactivity, enzyme responsible for N ‐methylation has remained elusive in most taxa. Here, we identified methyltransferase XrdM that is xenorhabdin (XRD) methylation Xenorhabdus doucetiae but encoded outside XRD gene cluster. turned out isofunctional with recently...
Abstract Dithiolopyrrolone (DTP) natural products are produced by several different bacteria and have potent antibacterial, antifungal anticancer activities. While the amide of their DTP core can be methylated to fine‐tune bioactivity, enzyme responsible for N ‐methylation has remained elusive in most taxa. Here, we identified methyltransferase XrdM that is xenorhabdin (XRD) methylation Xenorhabdus doucetiae but encoded outside XRD gene cluster. turned out isofunctional with recently...
Die gezielte Aktivierung von Biosynthesegenclustern in Δhfq-Mutanten verschiedener Proteobakterien vereinfacht die Isolierung und biologische Testung der dadurch exklusiv produzierten Naturstoffe, wie E. Bode et al. ihrem Forschungsartikel auf S. 19133 zeigen. easyPACId (“Easy promoter activated compound identification”) aktiviert auch stille Gencluster, durch geschlossenen Päckchen am Adventskalender symbolisiert sind, ermöglicht so Bestimmung Struktur biologischer Aktivität entsprechenden...