The plant growth promoting model bacterium FZB42T was proposed as the type strain of B. amyloliquefaciens subsp. plantarum (Borriss et al., Int J Syst Evol Microbiol. 2011, vol. 61, 1786-801), but has been recently recognized being synonymous to Bacillus velezensis due phylogenomic analysis (Dunlap al. 2016, 66, 1212-1217). However, until now, majority publications consider plant-associated close relatives FZB42 still ´B. amyloliquefaciens´. Here, we reinvestigated taxonomic status and...

The whole-genome-sequenced rhizobacterium Bacillus amyloliquefaciens FZB42(T) (Chen et al., 2007) and other plant-associated strains of the genus described as belonging to species or subtilis are used commercially promote growth improve health crop plants. Previous investigations revealed that a group represented distinct ecotype related B. amyloliquefaciens; however, exact taxonomic position this remains elusive (Reva 2004). In present study, we demonstrated ability closely strain colonize...

Crown gall disease (CGD), which is caused by the bacterium Agrobacterium, a common plant that often results in significant economic losses. Biocontrol offers an environmentally friendly and sustainable method to control CGD. In this study, we investigated biocontrol effect mechanism of CGD Japanese cherry trees B. velezensis FZB42, prototype strain growth promoting rhizobacteria (PGPR). We found FZB42 mutants unable produce one or combination four antimicrobial compounds (surfactin,...

Plants have developed a wide-range of adaptations to overcome nutrient limitation, including changes the quantity and composition carbon-containing compounds released by roots. Root-associated bacteria are largely influenced these which can be perceived as signals or substrates. Here, we evaluate effect root exudates collected from maize plants grown under nitrogen (N), phosphate (P), iron (Fe) potassium (K) deficiencies on transcriptome plant growth promoting rhizobacterium (PGPR) Bacillus...

Plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR exudates, we investigated changes the transcriptome a exudates.Bacillus amyloliquefaciens FZB42 well-studied PGPR. obtain comprehensive overview gene expression response...

Soybean root rot caused by the oomycete Phytophthora sojae is a serious soilborne disease threatening soybean production in China. Bacillus velezensis FZB42 model strain for Gram-positive plant growth-promoting rhizobacteria and able to produce multiple antibiotics. In this study, we demonstrated that B. can efficiently antagonize P. sojae. The underlying mechanism inhibition was then investigated. mutants deficient synthesis of lipopeptides (bacillomycin D fengycin), known have antifungal...

Crown gall disease (CGD), caused by Agrobacterium tumefaciens, is a common plant that leads to significant economic losses. Biological control sustainable and scalable method for managing CGD. In this study, we isolated three Bacillus strains from the rhizosphere soil of healthy cherry trees investigated their biocontrol activities underlying mechanisms against CGD trees. The results demonstrate can effectively inhibit growth pathogenic A. tumefaciens strain XYT58 in vitro under different...

Postharvest tomato fruits are cold-sensitive, and easy to develop chilling injury (CI) at cold storage. Information on physiological genetic characteristics closely related CI is necessary because it significant realize development regular find sensitive indicators reflecting cultivar tolerance, which the basics prevent CI. In this study, we used 2 cultivars (Lichun Santiam) differing in tolerance analyze ion leakage, malondialdehyde (MDA), proline, catalase (CAT), peroxidase (POD)...

Abstract Grapevine (Vitis vinifera L.,) is among the world’s leading fruit crops. The production of grapes severely affected by many diseases including gray mold, caused necrotrophic fungus Botrytis cinerea. Although all Vitis species can be hosts for B. cinerea, V. are particularly susceptible. Accordingly, this disease poses a significant threat to grape industry and causes substantial economic losses. Development resistant cultivars has progressed from incidental selection farmers,...

Pine wilt disease (PWD) caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, is one of most devastating diseases Pinus spp.The PWN was therefore listed as dangerous forest pests in China meriting quarantine.Virulence closely linked with spread PWD.However, main factors responsible for virulence PWNs are still unclear.Recently epiphytic bacteria carried have drawn much attention.But little known about relationship between endophytic and B. xylophilus.In this research, ten...

Abstract Surface‐enhanced Raman scattering (SERS) is widely used in all kinds of detection due to its ultrahigh sensitivity and selectivity. Micromotors, when as SERS sensors, or the so‐called “hotspots on fly”, can combine both controlled mobility sensing capacity, are ideal for versatile situ detection. In this work, mobile sensors successfully fabricated by growing gold nanospikes onto magnetic microsphere surfaces. These micromotors act normal characterized trace thiram, a highly toxic...

Colonization studies previously performed with a green-fluorescent-protein, GFP, labeled derivative of Bacillus amyloliquefaciens FZB42 revealed that the bacterium behaved different in colonizing surfaces plant roots species (Fan et al., 2012). In order to extend these and elucidate which genes are crucial for root colonization, we applied targeted mutant strains Arabidopsis seedlings. The fates colonization impaired synthesis alternative sigma factors, non-ribosomal lipopeptides...

Survival and infection of pathogenic bacteria, such as Staphylococcus aureus (S. aureus), pose a serious threat to human health. Efficient methods for recognizing quantifying low levels bacteria are imperiously needed. Herein, we introduce metal-organic framework (MOF)-based fluorescence resonance energy transfer (FRET) nanoprobe ratiometric detection S. aureus. The utilizes blue-emitting 7-hydroxycoumarin-4-acetic acid (HCAA) encapsulated inside zirconium (Zr)-based MOFs the donor...

Bacillus amyloliquefaciens ssp. plantarum FZB42 represents the prototype of Gram-positive plant growth promoting and biocontrol bacteria. In this study, we applied transposon mutagenesis to generate a library, which was screened for genes involved in multicellular behavior biofilm formation on roots as prerequisite activity. Transposon insertion sites were determined by rescue-cloning followed DNA sequencing. As B. subtilis, global transcriptional regulator DegU identified an activator...

Bacillus velezensis is one of the most widely applied bacteria in biofertilizers China and Europe. Additionally, molecular mechanisms plant growth promotion disease suppression by representative model strains are well established, such as B. SQR9 FZB42.

Plant-parasitic nematodes significantly threaten agriculture and forestry, causing various diseases. They cause annual losses of up to 178 billion dollars worldwide due their parasitism. Nematophagous fungi (NF) are valuable in controlling or reducing parasitic nematode diseases by killing through predatory behavior. This article summarizes the strategic approaches adopted NF capture, poison, consume for food. classified based on attacking strategies, including trapping, endoparasitism,...

The interfacial contact between the hole transport layer (HTL) and perovskite plays a critical role in determining power conversion efficiency (PCE) of solar cells (PSCs). Herein, to address limitations commercial NiOx nanoparticles realize low temperature fabrication compact film, chemical bath deposition (CBD) approach is employed strategically modified. By introducing an amino-alcohol ligand triisopropanolamine (TPA) into precursor, process effectively controlled. TPA binds strongly with...

Abstract Interfacial energy loss is a critical challenge in achieving high‐efficiency organic solar cells (OSCs), primarily due to mismatched levels and inefficient charge collection. Herein, bifunctional interface engineering strategy proposed, employing an ethanol/o‐difluorobenzene (EtOH/o‐DFB) dual‐solvent system for phosphotungstic acid (HPWO) processing. During film formation, o‐DFB regulates HPWO crystallization by suppressing excessive aggregation, while enabling situ ITO fluorination...

Bacillus amyloliquefaciens subsp. plantarum FZB42 is a representative of Gram-positive plant-growth-promoting rhizobacteria (PGPR) that inhabit plant root environments. In order to better understand the molecular mechanisms bacteria-plant symbiosis, we have systematically analyzed primary transcriptome strain grown under rhizosphere-mimicking conditions using differential RNA sequencing (dRNA-seq). Our analysis revealed 4,877 transcription start sites for protein-coding genes, identified...