A5101484708- Acute Ischemic Stroke Management
- Cerebrovascular and Carotid Artery Diseases
- Stroke Rehabilitation and Recovery
- Venous Thromboembolism Diagnosis and Management
- Pancreatic function and diabetes
- Traumatic Brain Injury and Neurovascular Disturbances
- Smart Grid Energy Management
- Microgrid Control and Optimization
- CRISPR and Genetic Engineering
- Acute Myeloid Leukemia Research
- RNA and protein synthesis mechanisms
- Antiplatelet Therapy and Cardiovascular Diseases
- RNA Research and Splicing
- Intracerebral and Subarachnoid Hemorrhage Research
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Frequency Control in Power Systems
- Control and Stability of Dynamical Systems
- Genomics and Phylogenetic Studies
- Diabetes and associated disorders
- Scoliosis diagnosis and treatment
- Heart Rate Variability and Autonomic Control
- Genomics and Chromatin Dynamics
- Insect symbiosis and bacterial influences
- Vector-borne infectious diseases
Hospital of the Brothers of St. John of God
2018-2025
Ludwig-Maximilians-Universität München
2012-2024
Population Health Research Institute
2024
McMaster University
2024
Medical University of Graz
2016-2023
Medical University of Vienna
2017-2023
Sigmund Freud University Vienna
2017-2023
St. John's Hospital
2022-2023
University of Bern
2023
St. Johns Hospital
2018-2022
During maternal-to-embryonic transition control of embryonic development gradually switches from maternal RNAs and proteins stored in the oocyte to gene products generated after genome activation (EGA). Detailed insight into onset transcription is obscured by presence transcripts. Using bovine model system, we established RNA sequencing a comprehensive catalogue transcripts germinal vesicle metaphase II oocytes, embryos at four-cell, eight-cell, 16-cell, blastocyst stages. These were...
Duchenne muscular dystrophy (DMD) is caused by mutations in the X-linked dystrophin gene. The absence of protein leads to progressive muscle weakness and wasting, disability death. To establish a tailored large animal model DMD, we deleted DMD exon 52 male pig cells gene targeting generated offspring nuclear transfer. pigs exhibit skeletal muscles, increased serum creatine kinase levels, dystrophic changes impaired mobility, maximum life span 3 months due respiratory impairment. Unlike human...
Coliform bacteria are the most common etiologic agents in severe mastitis of cows. Escherichia coli infections mostly restricted to a single udder quarter whereas neighboring quarters stay clinically inapparent, implicating presence systemic defense reaction. To address its underlying mechanism, we performed transcriptome study mammary tissue from inoculated with E. (6 h and 24 post infection), same animals, untreated control animals.After 6 13 probe sets differentially expressed genes (DEG)...
Mutations in the insulin (INS) gene may cause permanent neonatal diabetes mellitus (PNDM). Ins2 mutant mouse models provided important insights into disease mechanisms of PNDM but have limitations for translational research. To establish a large animal model PNDM, we generated INSC94Y transgenic pigs. A line expressing high levels mRNA (70–86% wild-type INS transcripts) exhibited elevated blood glucose soon after birth unaltered β-cell mass at age 8 days. At 4.5 months, pigs 41% reduced body...
Patients with ischaemic stroke or transient attack (TIA) are at high risk of recurrent and other cardiovascular diseases commonly suffer from reduced quality life. We aimed to determine whether the disease management programme STROKE-CARD can prevent improve life in these patients.In this pragmatic open-label two-centre randomised controlled trial blinded outcome assessment, we randomly assigned patients acute TIA (ABCD2 score ≥3) a 2:1 ratio receive care standard care. is by...
It is unclear whether IV thrombolysis (IVT) outperforms early dual antiplatelet therapy (DAPT) in the acute setting of mild ischemic stroke. The aim this study was to compare safety and efficacy IVT with that DAPT.
The aim of our study was to analyze endometrial gene expression profiles in ovariectomized cows treated with estradiol and/or progesterone by using microarray analysis. Clustering differentially expressed genes allowed separation into distinct hormone response patterns. These patterns could be classified independent and interdependent actions the steroid hormones estrogen progesterone. use external administration identified a set whose regulation depends on priming effect....
Ischemic strokes associated with atrial fibrillation (AF) are more severe than those of other cause. We aim to study potential sex effects in this context.In cross-sectional study, 74 425 adults acute ischemic stroke from the Austrian Stroke Unit Registry were included between March 2003 and January 2016. In 63 563 patients, data on National Institutes Health Scale admission unit, presence AF, vascular risk factors, comorbidities complete. Analysis was done by a multivariate regression...
Exploratory analysis of the phase 2 PACIFIC-Stroke (Program Anticoagulation via Inhibition FXIa by Oral Compound BAY 2433334-Non-Cardioembolic Stroke) randomized trial suggested that asundexian, an oral factor XIa inhibitor, prevents recurrent stroke and transient ischemic attacks in patients with atherosclerotic stroke. In this post hoc exploratory analysis, we hypothesized asundexian would be more effective enrolled large, multiple, or cortical acute infarcts on magnetic resonance imaging...
Diabetes is generally diagnosed too late. Therefore, biomarkers indicating early stages of β-cell dysfunction and mass reduction would facilitate timely counteraction. Transgenic pigs expressing a dominant-negative glucose-dependent insulinotropic polypeptide receptor (GIPRdn) reveal progressive deterioration glucose control mass, providing unique opportunity to study metabolic changes during the prediabetic period. Plasma samples from intravenous tolerance tests 2.5- 5-month-old GIPRdn...
The prevalence of diabetes mellitus and associated complications is steadily increasing. As a resource for studying systemic consequences chronic insulin insufficiency hyperglycemia, we established comprehensive biobank long-term diabetic INSC94Y transgenic pigs, model mutant INS gene-induced youth (MIDY), wild-type (WT) littermates.Female MIDY pigs (n = 4) were maintained with suboptimal treatment 2 years, together female WT littermates 5). Plasma insulin, C-peptide glucagon levels...
Nuclear landscapes were studied during preimplantation development of bovine embryos, generated either by in vitro fertilization (IVF), or as cloned embryos somatic cell nuclear transfer (SCNT) fetal fibroblasts, using 3-dimensional confocal laser scanning microscopy (3D-CLSM) and structured illumination (3D-SIM). IVF SCNT embryonic nuclei compared with each other fibroblast nuclei. We demonstrate that reprogramming requires changes their similar to embryos. On the way toward 8-cell stage,...
Up to 30% of stroke patients initially presenting with non-disabling or mild deficits may experience poor functional outcome. Despite, intravenous thrombolysis remains controversial in this subgroup due its uncertain risk benefit ratio.We aimed analyze the real-world very low NIHSS.Data initial severity (NIHSS 0-5) including vascular factors, syndrome and etiology, early neurological deterioration, symptomatic intracerebral haemorrhage (sICH), outcome by modified Rankin Scale were extracted...
Introduction: Evidence regarding the benefit of endovascular therapy (EVT) in patients with acute ischemic stroke (AIS) due to isolated cervical internal carotid artery occlusion (c-ICA-O) is lacking. We assessed outcomes and safety EVT c-ICA-O. Methods: Retrospective multicenter cohort study an AIS c-ICA-O, within 24-h since last-seen-well. Comparisons were made between best medical (BMT). The primary outcome was 3-months modified Rankin Scale (mRS) ordinal shift. Secondary included 3-month...
Whether thrombectomy compared with best medical treatment (BMT) improves outcome in patients stroke and carotid artery dissection (CAD) is unknown. This was an international observational study based on prospective nationwide Austrian, German, Swiss registries. Patients large vessel occlusion (LVO) due to CAD were according modality (thrombectomy vs BMT including intravenous thrombolysis) admission severity NIH Stroke Scale (NIHSS) <6 NIHSS ≥6. The primary the favorable functional (modified...
The liver regulates the availability of insulin to other tissues and is first line response organ physiologically exposed higher concentrations than periphery. Basal during fasting inhibits hepatic gluconeogenesis glycogenolysis, whereas postprandial peaks stimulate glycogen synthesis. molecular consequences chronic deficiency for have not been studied systematically. We analyzed samples a genetically diabetic pig model (MIDY) wild-type (WT) littermate controls by RNA sequencing, proteomics,...
MALDI mass spectrometry is an established platform for high‐throughput genotyping of single nucleotide polymorphisms (SNPs). For many species and also specific ethnic groups, the number described SNPs far from sufficient. Here we present a method SNP discovery that can use existing platforms automation‐compatible. The based on in vitro RNA transcripts PCR products, be used to obtain highly informative sequence fingerprints by digestion with guanosine‐ ribonuclease T1. In these fingerprints,...
<h3>Objective:</h3> It is not clear whether risk scores for early stroke recurrence after TIA that have been mainly established in outpatient and emergency department settings are valid on the background of highly specialized unit care. <h3>Methods:</h3> ABCD2 ABCD3-I prospectively documented a cohort patients admitted to Austrian units within 24 hours symptom onset with or minor (NIH Stroke Scale score <4). <h3>Results:</h3> A total 5,237 met inclusion criteria, 3-month follow-up data...
Patients with ischaemic stroke or transient attack (TIA) are at high risk of future cardiovascular events. Despite compelling evidence about the efficacy secondary prevention, a substantial gap exists between factor management in real life and that recommended by international guidelines. Moreover, is leading cause disability morbidity which partly emerges from post-stroke complications. We designed block-randomised (2:1 ratio) open pragmatic trial [NCT02156778] blinded outcome assessment...
The present study demonstrates a major remodeling of the nuclear envelope and its underlying lamina during bovine preimplantation development. Up to onset embryonic genome activation (MGA) at 8-cell stage nuclei showed non-uniform distribution pore complexes (NPCs). NPCs were exclusively sites where DNA contacted lamina. Extended regions lamina, which not by DNA, lacked NPCs. In post-MGA whole was rather uniformly DNA. Accordingly, became distributed throughout entire envelope. These...
The glucagon-like peptide-1 receptor (GLP1R) agonist liraglutide improves glycemic control and reduces body weight of adult type 2 diabetic patients. However, efficacy safety in adolescents has not been systematically investigated. Furthermore, possible pro-proliferative effects GLP1R agonists on the endocrine exocrine pancreas need to be further evaluated. We studied adolescent pigs expressing a dominant-negative glucose-dependent insulinotropic polypeptide (GIPRdn) beta-cells, leading...
Changing definition of TIA from time to a tissue basis questions the validity well-established ABCD3-I risk score for recurrent ischemic cerebrovascular events. We analyzed patients with stroke mild neurological symptoms arriving < 24 h after symptom onset in phase where it is unclear, if event turns out be or minor stroke, prospective multi-center Austrian Stroke Unit Registry. Patients were retrospectively categorized according time-based (symptom duration below/above h) and tissue-based...