Jochen Graw
A5064155313- Connexins and lens biology
- Retinal Development and Disorders
- Intraocular Surgery and Lenses
- Glaucoma and retinal disorders
- Ocular Disorders and Treatments
- Yersinia bacterium, plague, ectoparasites research
- Biochemical effects in animals
- Hemophilia Treatment and Research
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Heat shock proteins research
- Animal Genetics and Reproduction
- Aldose Reductase and Taurine
- RNA regulation and disease
- Retinal Diseases and Treatments
- Developmental Biology and Gene Regulation
- Corneal Surgery and Treatments
- melanin and skin pigmentation
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Bacterial Genetics and Biotechnology
- Calpain Protease Function and Regulation
- Effects of Radiation Exposure
Helmholtz Zentrum München
2013-2022
Institute of Groundwater Ecology
1999-2022
Martin Luther University Halle-Wittenberg
2020
München Klinik
2019
Center for Environmental Health
2005-2013
Institute of Developmental Physiology
2008-2012
University of Ferrara
2011
Max Planck Institute of Psychiatry
2011
Imperial College London
2011
Amt für Umwelt
2001-2008
MENX is a recessive multiple endocrine neoplasia-like syndrome in the rat. The tumor spectrum overlaps those of human neoplasia (MEN) types 1 and 2. We mapped MenX locus to distal part rat chromosome 4, excluding homologs genes responsible for MEN syndromes ( RET MEN1 ) with an component VHL NF1 ). report fine mapping disease identification homozygous frameshift mutation Cdkn1b , encoding cyclin-dependent kinase inhibitor p27 Kip1 . As consequence mutation, MENX-affected rats show dramatic...
The mouse inbred line C57BL/6J is widely used in genetics and its genome has been incorporated into many genetic reference populations. More recently large initiatives such as the International Knockout Mouse Consortium (IKMC) are using C57BL/6N strain to generate null alleles for all genes. Hence both strains now studies. Here we perform a comprehensive genomic phenotypic analysis of two identify differences that may influence their underlying mechanisms.We undertake sequence comparisons...
Aging is a major risk factor for large number of disorders and functional impairments. Therapeutic targeting the aging process may therefore represent an innovative strategy in quest novel broadly effective treatments against age-related diseases. The recent report lifespan extension mice treated with FDA-approved mTOR inhibitor rapamycin represented first demonstration pharmacological maximal mammals. Longevity effects may, however, be due to rapamycin’s on specific life-limiting...
The mesencephalic dopamine (mesDA) system is involved in the control of movement and behavior. expression Pitx3 brain restricted to mesDA gene induced relatively late, at E11.5, a time when tyrosine hydroxylase (Th) initiated. We show here that, Pitx3-deficient aphakia(ak) mouse mutant, malformed. Owing developmental failure neurons lateral field midbrain,mesDA are not found SNc projections caudate putamen selectively lost. However, expressed all animals. Therefore, react specifically loss...
The transcription factor Pax6 plays a key role during development of various organs, including the brain where it affects cell fate, proliferation and patterning. To understand how coordinates these diverse effects at molecular level, we examined distinct DNA-binding domains Pax6, homeodomain (HD), paired domain (PD) its splice variant (5a), using loss- gain-of-function approaches. Here show that PD is necessary for regulation neurogenesis, patterning since aspects are severely affected in...
Mutations in the insulin (INS) gene may cause permanent neonatal diabetes mellitus (PNDM). Ins2 mutant mouse models provided important insights into disease mechanisms of PNDM but have limitations for translational research. To establish a large animal model PNDM, we generated INSC94Y transgenic pigs. A line expressing high levels mRNA (70–86% wild-type INS transcripts) exhibited elevated blood glucose soon after birth unaltered β-cell mass at age 8 days. At 4.5 months, pigs 41% reduced body...
Dietary restriction regimes extend lifespan in various animal models. Here we show that longevity male C57BL/6J mice subjected to every-other-day feeding is associated with a delayed onset of neoplastic disease naturally limits these animals. We compare more than 200 phenotypes over 20 tissues aged animals fed lifelong or ad libitum access food diet determine whether molecular, cellular, physiological and histopathological aging features develop slowly controls. also analyze the effects on...
ADAR2, an RNA editing enzyme that converts specific adenosines to inosines in certain pre-mRNAs, often leading amino acid substitutions the encoded proteins, is mainly expressed brain. Of all ADAR2-mediated edits, a single one pre-mRNA of AMPA receptor subunit GluA2 essential for survival. Hence, early postnatal death mice lacking ADAR2 averted when critical edit engineered into both encoding Gria2 alleles. Adar2−/−/Gria2R/R display normal appearance and life span, but general phenotypic...
Animal welfare requires the adequate housing of animals to ensure health and well-being. The application environmental enrichment is a way improve well-being laboratory animals. However, it important know whether these items can be incorporated in experimental mouse husbandry without creating divide between past future results. Previous small-scale studies have been inconsistent throughout literature, not yet completely understood how might endanger comparability results scientific...
Abstract Current concepts regarding the biology of aging are primarily based on studies aimed at identifying factors regulating lifespan. However, lifespan as a sole proxy measure for can be limited value because it may restricted by specific pathologies. Here, we employ large-scale phenotyping to analyze hundreds markers in male C57BL/6J mice. For each phenotype, establish lifetime profiles determine when age-dependent change is first detectable relative young adult baseline. We examine key...