A5085113673- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Axon Guidance and Neuronal Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Anesthesia and Neurotoxicity Research
- Congenital heart defects research
- Microtubule and mitosis dynamics
- Genetics and Neurodevelopmental Disorders
- Nerve injury and regeneration
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Hedgehog Signaling Pathway Studies
- Wnt/β-catenin signaling in development and cancer
- Genomics and Chromatin Dynamics
- Immune cells in cancer
- RNA modifications and cancer
Ludwig-Maximilians-Universität München
2016-2025
Helmholtz Zentrum München
2016-2025
Munich Cluster for Systems Neurology
2016-2025
Urologische Klinik München
2021-2025
University Hospital Carl Gustav Carus
2024
TU Dresden
2024
Institute of Bioinformatics and Systems Biology
2018-2022
Genomics (United Kingdom)
2011-2021
Max Planck Institute of Biochemistry
2019-2020
Center for Environmental Health
2013-2018
The developing central nervous system of vertebrates contains an abundant cell type designated radial glial cells. These cells are known as guiding cables for migrating neurons, while their role precursor is less clear. Since express a variety astroglial characteristics and differentiate astrocytes after completing guidance function, they have been considered part the lineage. Using fluorescence-activated sorting, we show here that also neuronal precursors only later, neurogenesis, do shift...
Reactive gliosis is the universal reaction to brain injury, but precise origin and subsequent fate of glial cells reacting injury are unknown. Astrocytes react by hypertrophy up-regulation glial-fibrillary acidic protein (GFAP). Whereas mature astrocytes do not normally divide, a subpopulation reactive GFAP(+) does so, prompting question whether proliferating arise from endogenous progenitors or that start proliferate in response injury. Here we show genetic mapping cell type-specific viral...
Abstract Little is known about how neurons in the different layers of mammalian cerebral cortex are specified at molecular level. Expression two homologues Drosophila homeobox Cut gene, Cux‐1 and Cux‐2 , strikingly specific to pyramidal upper (II–IV) murine cortex, suggesting that they may define identity these neurons. An antibody against labels nucleus most postmitotic layer but does not label parvoalbumin‐positive cortical interneurons derive from medial ganglionic eminence. represent...
Despite their abundance, still little is known about the rather frequent, constantly proliferating progenitors spread throughout adult mouse brain parenchyma. The majority of these express basic-helix-loop-helix transcription factor Olig2, and number further increases after injury. Here, we examine progeny this progenitor population by genetic fate mapping using tamoxifen-inducible Cre-recombination in Olig2 locus to turn on permanent reporter gene expression brain. Consistent with NG2(+)...
Astroglia from the postnatal cerebral cortex can be reprogrammed in vitro to generate neurons following forced expression of neurogenic transcription factors, thus opening new avenues towards a potential use endogenous astroglia for brain repair. However, previous attempts astroglia-derived failed establish functional synapses, severe limitation neurogenesis. It remained therefore also unknown whether derived could directed distinct neuronal subtype identities by selective fate determinants....
Astrocytes are thought to play a variety of key roles in the adult brain, such as their participation synaptic transmission, wound healing upon brain injury, and neurogenesis. However, elucidate these functions vivo has been difficult because lack astrocyte-specific gene targeting. Here we show that inducible form Cre (CreERT2) expressed locus glutamate transporter (GLAST) allows precisely timed deletion astrocytes well radial glial cells at earlier developmental stages. Moreover, postnatal...
New neurons in the adult dentate gyrus are widely held to incorporate into hippocampal circuitry via a stereotypical sequence of morphological and physiological transitions, yet molecular control over this process remains unclear. We studied role brain-derived neurotrophic factor (BDNF)/TrkB signaling neurogenesis by deleting full-length TrkB Cre expression within progenitors TrkB(lox/lox) mice. By 4 weeks after deletion, growth dendrites spines is reduced adult-born demonstrating that...
Despite the presence of neural stem cells and ongoing neurogenesis in some regions adult mammalian brain, neurons are not replaced most brain after injury. With aim to unravel factors contributing failure injured cerebral cortex, we examined expression cell fate determinants acute injuries, such as stab wound or focal ischemia, a model chronic amyloid deposition. Although none neurogenic factors, Pax6, Mash1, Ngn2, was detected parenchyma, observed strong up-regulation bHLH transcription...
With the exception of astroglia-like cells in neurogenic niches telencephalic subependymal or hippocampal subgranular zone, astroglia all other regions adult mouse brain do not normally generate neurons. Previous studies have shown, however, that early postnatal cortical culture can be reprogrammed to adopt a neuronal fate after forced expression Pax6, transcription factor (TF) required for proper specification during embryonic corticogenesis. Here we show also proneural genes neurogenin-2...