A5049217323- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Anesthesia and Neurotoxicity Research
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Glioma Diagnosis and Treatment
- Axon Guidance and Neuronal Signaling
- Epilepsy research and treatment
- Hedgehog Signaling Pathway Studies
- Memory and Neural Mechanisms
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Transcranial Magnetic Stimulation Studies
- Cancer Cells and Metastasis
- Asthma and respiratory diseases
- RNA regulation and disease
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Planarian Biology and Electrostimulation
Inserm
2011-2025
Institut Cellule Souche et Cerveau
2018-2025
Université Claude Bernard Lyon 1
2018-2025
Grenoble Institute of Neurosciences
2014-2022
Université Grenoble Alpes
2011-2022
Ludwig-Maximilians-Universität München
2010-2015
Université Joseph Fourier
2005-2015
Centre Hospitalier Universitaire de Grenoble
2008-2015
Zero to Three
2011
CEA Grenoble
2008-2011
Astroglia from the postnatal cerebral cortex can be reprogrammed in vitro to generate neurons following forced expression of neurogenic transcription factors, thus opening new avenues towards a potential use endogenous astroglia for brain repair. However, previous attempts astroglia-derived failed establish functional synapses, severe limitation neurogenesis. It remained therefore also unknown whether derived could directed distinct neuronal subtype identities by selective fate determinants....
The adult cerebral cortex lacks the capacity to replace degenerated neurons following traumatic injury. Conversion of nonneuronal cells into induced has been proposed as an innovative strategy toward brain repair. Here, we show that retrovirus-mediated expression transcription factors Sox2 and Ascl1, but strikingly also alone, can induce conversion genetically fate-mapped NG2 glia doublecortin (DCX)(+) in mouse stab wound injury vivo. In contrast, lentiviral unlesioned failed convert...
Mesio-temporal lobe epilepsy (MTLE) is often accompanied by granule cell dispersion (GCD), a widening of the layer. The molecular determinants GCD are poorly understood. Here, we used an animal model to study whether results from increased dentate neurogenesis associated with abnormal migration newly generated cells. Adult mice were given intrahippocampal injections kainate (KA) known induce focal epileptic seizures and GCD, comparable changes observed in human MTLE. Ipsilateral...
Purpose: Neuroinflammation appears as a prominent feature of the mesiotemporal lobe epilepsy syndrome (MTLE) that is observed in human patients and animal models. However, precise temporal relationship its development during epileptogenesis remains to be determined. The aim present study was investigate (1) time course spatial distribution neuronal death associated with seizure development, (2) microglia astrocyte activation, (3) kinetics induction mRNAs from neuroinflammatory-related...
Reprogramming brain-resident glial cells into clinically relevant induced neurons (iNs) is an emerging strategy toward replacing lost and restoring brain functions. A fundamental question now whether iNs can promote functional recovery in pathological contexts. We addressed this the context of therapy-resistant mesial temporal lobe epilepsy (MTLE), which associated with hippocampal seizures degeneration GABAergic interneurons. Using a MTLE mouse model, we show that retrovirus-driven...
The ventricular-subventricular zone (V-SVZ) is the largest neurogenic region of postnatal forebrain, containing neural stem cells (NSCs) that emerge from both embryonic pallium and subpallium. Despite this dual origin, glutamatergic neurogenesis declines rapidly after birth, while GABAergic persists throughout life. We performed single-cell RNA sequencing dorsal V-SVZ for unraveling mechanisms leading to pallial lineage germinal activity silencing. show NSCs enter a state deep quiescence,...
ABSTRACT A major challenge to study the regenerative potential of injured brain is limited access this organ in vivo . To address this, we developed an innovative gliosis model that, although established vitro , originates from a genuine injury The relies on reactive glia acquiring enhanced adhesion, facilitating their rapid adaptation conditions, where it faithfully recapitulates key features injury. These include secretome associated with pathways, degenerative responses like neuronal...
Severe and medication-resistant psychiatric diseases, such as major depressive disorder, bipolar disorder or schizophrenia, can be effectively rapidly treated by electroconvulsive therapy (ECT). Despite extensive long-standing clinical use, the neurobiological mechanisms underlying curative action of ECT remain incompletely understood.Unravel biological basis stimulation (ECS) efficacy, animal equivalent ECT.Using MAP6 KO mouse, a genetic model that constitutively exhibits features relevant...
The largest diversity of neural lineages generated from the subventricular zone (SVZ) occurs early after birth and is regulated in a spatiotemporal manner depending on expression specific transcriptional cues. Transcriptomics fate-mapping approaches were employed to explore relationship between regional transcription factors by stem cells (NSCs) specification distinct lineages. Our results support an priming NSCs for genesis defined cell types their spatial location SVZ identify HOPX as...
In the subependymal zone and dentate gyrus of adult brain rodents, neural stem cells with glial properties generate new neurons in a life-long process. The identification progenitors outside neurogenic niches, oligodendrocyte precursors healthy brain, reactive astrocytes after cortical injury led to idea using these as endogenous cell source for repair cerebral cortex. Recently, our group showed that proliferating astroglia from cortex can be reprogrammed into capable action potential firing...
Summary: Purpose: Unilateral intrahippocampal injection of kainic acid (KA) in adult mice induces the progressive dispersion dentate granule cells, one characteristic pathologic changes mesial temporal lobe epilepsy. However, little is known about mechanisms that trigger this dispersion. In study, possible involvement glutamatergic and γ‐aminobutyric (GABA)ergic neurotransmissions development cell (GCD) was examined model. Methods: Antagonists N ‐methyl‐ d ‐aspartate (NMDA) receptor (MK‐801)...
Granule cell dispersion (GCD) appears as a characteristic morphological feature of the mesial temporal lobe epilepsy (MTLE). It has been suggested that this phenomenon could be due to an increased neurogenesis in dentate gyrus. However, hypothesis is still debated and recent clinical experimental studies have shown rather decreased MTLE. To further determine role neural astroglial generation GCD we examined consequences aging irradiation, which are known reduce progenitor cells, mouse model...
Germinal activity persists throughout life within the ventricular-subventricular zone (V-SVZ) of postnatal forebrain due to presence neural stem cells (NSCs). Accumulating evidence points a recruitment for these following early brain injuries and suggests their amenability manipulations. We used chronic hypoxia as rodent model injury investigate reactivation cortical progenitors at times. Our results reveal an increased proliferation production glutamatergic dorsal V-SVZ. Fate mapping V-SVZ...
Absence epilepsy belongs to genetic epilepsies and is characterized by recurrent generalized seizures that are concomitant with alterations of consciousness associated cognitive comorbidities. Little known about the mechanisms leading occurrence epileptic (i.e. epileptogenesis) and, in particular, it remains an open question as whether neuronal hypersynchronization, a key feature seizure initiation, could result from aberrant structural connectivity within networks endowing them properties....