A5087508483- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Zebrafish Biomedical Research Applications
- 3D Printing in Biomedical Research
- Cancer Treatment and Pharmacology
- Lung Cancer Treatments and Mutations
- Gene Regulatory Network Analysis
- CAR-T cell therapy research
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Immunotherapy and Immune Responses
- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
ETH Zurich
2015-2024
Board of the Swiss Federal Institutes of Technology
2016-2024
Helmholtz Zentrum München
2010-2019
Ludwig-Maximilians-Universität München
2015
Max Planck Institute of Molecular Cell Biology and Genetics
2015
Max Planck Institute for Plant Breeding Research
2015
Center for Environmental Health
2013
École Polytechnique Fédérale de Lausanne
2013
Institute of Molecular Genetics
2008
Institute of Groundwater Ecology
2005-2007
Astroglia from the postnatal cerebral cortex can be reprogrammed in vitro to generate neurons following forced expression of neurogenic transcription factors, thus opening new avenues towards a potential use endogenous astroglia for brain repair. However, previous attempts astroglia-derived failed establish functional synapses, severe limitation neurogenesis. It remained therefore also unknown whether derived could directed distinct neuronal subtype identities by selective fate determinants....
The constant regeneration of the blood system during hematopoiesis requires tightly controlled lineage decisions hematopoietic progenitor cells (HPCs). Because technical limitations, differentiation individual HPCs could not previously be analyzed continuously. It was therefore disputed whether cell-extrinsic cytokines can instruct HPC choice or only allow survival that are already lineage-restricted. Here, we used bioimaging approaches continuous long-term observation differentiating mouse...
With the exception of astroglia-like cells in neurogenic niches telencephalic subependymal or hippocampal subgranular zone, astroglia all other regions adult mouse brain do not normally generate neurons. Previous studies have shown, however, that early postnatal cortical culture can be reprogrammed to adopt a neuronal fate after forced expression Pax6, transcription factor (TF) required for proper specification during embryonic corticogenesis. Here we show also proneural genes neurogenin-2...
Abstract Quantitative analysis of bioimaging data is often skewed by both shading in space and background variation time. We introduce BaSiC, an image correction method based on low-rank sparse decomposition which solves issues. In comparison to existing tools, BaSiC achieves high-accuracy with significantly fewer input images, works for diverse imaging conditions robust against artefacts. Moreover, it can correct temporal drift time-lapse microscopy thus improve continuous single-cell...
Induced pluripotent stem cells (iPSCs) can be derived from somatic by gene transfer of reprogramming transcription factors. Expression levels these factors strongly influence the overall efficacy to form iPSC colonies, but additional contribution stochastic cell-intrinsic has been proposed. Here, we present engineered color-coded lentiviral vectors in which codon-optimized are co-expressed a strong retroviral promoter that is rapidly silenced iPSC, and imaged conversion fibroblasts iPSC. We...
Circulating tumor cells (CTCs) are shed from solid cancers in the form of single or clustered cells, and latter display an extraordinary ability to initiate metastasis. Yet, biological phenomena that trigger shedding CTC clusters a primary cancerous lesion poorly understood. Here, when dynamically labeling breast cancer along progression, we observe majority undergoing hypoxia, while CTCs largely normoxic. Strikingly, find vascular endothelial growth factor (VEGF) targeting leads shrinkage,...
Similarities as well differences in higher order chromatin arrangements of human cell types were previously reported. For an evolutionary comparison, we now studied the chromosome territories and centromere regions six mouse (lymphocytes, embryonic stem cells, macrophages, fibroblasts, myoblasts myotubes) with fluorescence situ hybridization confocal laser scanning microscopy. Both species evolved pronounced karyotypes after their last common ancestors lived about 87 million years ago thus...
The size of brain regions depends on the balance between proliferation and differentiation. During development mouse cerebral cortex, ventricular zone (VZ) progenitors, neuroepithelial radial glial cells, enlarge progenitor pool by proliferative divisions, while basal progenitors located in subventricular (SVZ) mostly divide a differentiative mode generating two neurons. These differences correlate to existence an apico-basal polarity VZ, but not SVZ, progenitors. Only VZ possess apical...