A5073200002- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Pancreatic function and diabetes
- Neonatal Respiratory Health Research
- Cell death mechanisms and regulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Air Quality and Health Impacts
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- MicroRNA in disease regulation
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- T-cell and B-cell Immunology
- Digestive system and related health
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Inhalation and Respiratory Drug Delivery
- Diabetes and associated disorders
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
Helmholtz Zentrum München
2016-2025
Center for Environmental Health
2010-2025
Heinrich Heine University Düsseldorf
2013-2021
German Center for Diabetes Research
2013-2021
Deutsches Diabetes-Zentrum e.V.
2013-2021
Deutsches Biomasseforschungszentrum
2020
Ludwig-Maximilians-Universität München
2016
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2016
Weihenstephan-Triesdorf University of Applied Sciences
2013
Czech Academy of Sciences, Institute of Experimental Medicine
2009-2011
Members of the tumor necrosis factor (TNF) family induce pleiotropic biological responses, including cell growth, differentiation, and even death. Here we describe a novel member TNF designated APRIL (for proliferation-inducing ligand). Although transcripts are low abundance in normal tissues, high levels mRNA detected transformed lines, human cancers colon, thyroid, lymphoid tissues vivo. The addition recombinant to various cells stimulates their proliferation. Moreover, APRIL-transfected...
Fibroblast-to-myofibroblast conversion is a major driver of tissue remodelling in organ fibrosis. Distinct lineages fibroblasts support homeostatic niche functions, yet their specific activation states and phenotypic trajectories during injury repair have remained unclear.
FLICE-inhibitory protein, FLIP (Casper/I-FLICE/FLAME-1/CASH/CLARP/MRIT), which contains two death effector domains and an inactive caspase domain, binds to FADD caspase-8, thereby inhibits receptor-mediated apoptosis. Here, we characterize the inhibitory effect of on a variety apoptotic pathways. Human Jurkat T cells undergoing Fas ligand-mediated apoptosis in response CD3 activation were completely resistant when transfected with FLIP. In contrast, presence did not affect induced by...
Apoptosis is critically dependent on the presence of ced-3 gene in Caenorhabditis elegans, which encodes a protein homologous to mammalian interleukin (IL)-1 beta-converting enzyme (ICE). Overexpression ICE or promotes apoptosis. Cytotoxic T lymphocyte-mediated rapid apoptosis induced by proteases granzyme A and B. B share rare substrate site aspartic acid, after amino acid cleavage precursor IL-1 beta (pIL-1 beta) occurs. Here we show that A, but not B, converts pIL-1 its 17-kD mature form....
Abstract Fas ligand (FasL, Apo‐1L) is a member of the tumor necrosis factor protein family and binding to its receptor (Fas, Apo‐1, CD95) triggers cell death through apoptosis. Ligand expression restricted cells with known cytolytic activity found on hematopoietic T natural killer lineage. Here we provide evidence that B lymphocytes can express FasL. Flow cytometric analysis revealed FasL expressed surface upon stimulation either lipopolysaccharide or phorbol 12‐myristute...
Bcl-2 is a mitochondrial- and perinuclear-associated protein that prolongs the lifespan of variety cell types by interfering with programmed death (apoptosis). seems to function in an antioxidant pathway, it believed membrane attachment mediated COOH-terminal hydrophobic tail required for its full activity. To identify critical regions bcl-2 alpha subcellular localization, activity, and/or interaction other proteins, we created, site-directed mutagenesis, various deletion, truncation, point...
Direct lineage reprogramming induces dramatic shifts in cellular identity, employing poorly understood mechanisms. Recently, we demonstrated that expression of Neurog2 or Ascl1 postnatal mouse astrocytes generates glutamatergic GABAergic neurons. Here, take advantage this model to study dynamics neuronal cell fate acquisition at the transcriptional level. We found and rapidly elicited distinct neurogenic programs with only a small subset shared target genes. Within subset, NeuroD4 could by...
Master regulators of the epithelial-mesenchymal transition such as Twist1 and Snail1 have been implicated in invasiveness generation cancer stem cells, but their persistent activity inhibits stem-cell-like properties outgrowth disseminated cells into macroscopic metastases. Here, we show that activation primes a subset mammary epithelial for properties, which only emerge stably persist following deactivation. Consequently, when undergo mesenchymal-epithelial (MET), they do not return to...
BACKGROUND. Dietary intake of saturated fat is a likely contributor to nonalcoholic fatty liver disease (NAFLD) and insulin resistance, but the mechanisms that initiate these abnormalities in humans remain unclear. We examined effects single oral load on sensitivity, hepatic glucose metabolism, lipid metabolism humans. Similarly, initiating were after an equivalent challenge mice.
We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured adherent or floating collagen gels, corresponding to a rigid compliant matrix environment. In both conditions, luminal progenitors form spheres, whereas basal generate branched ductal structures. but not branching ducts alveoli at their tips, express and markers correct positions, display contractility, is required for alveologenesis. Thereby, structures generated gels resemble...
Astrocytes react to brain injury in a heterogeneous manner with only subset resuming proliferation and acquiring stem cell properties vitro. In order identify novel regulators of this subset, we performed genomewide expression analysis reactive astrocytes isolated 5 days after stab wound from the gray matter adult mouse cerebral cortex. The pattern was compared intact cortex neural cells (NSCs) subependymal zone (SEZ). These comparisons revealed set genes expressed at higher levels both...
The choroid plexuses (ChPs) are the main regulators of cerebrospinal fluid (CSF) composition and thereby also control a principal source signaling molecules that is in direct contact with neural stem cells developing brain. ChP development mediating acquisition fate differs from neighboring neuroepithelial poorly understood. Here, we demonstrate mice crucial role for transcription factor Otx2 maintenance cells. Deletion by Otx2-CreERT2 driver line at E9 resulted lack all ChPs, whereas...