A5060118711- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Memory and Neural Mechanisms
- Genetics and Neurodevelopmental Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Stress Responses and Cortisol
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Spaceflight effects on biology
- Anesthesia and Neurotoxicity Research
- Epigenetics and DNA Methylation
- Radiation Therapy and Dosimetry
- Neuroendocrine regulation and behavior
- MicroRNA in disease regulation
- Axon Guidance and Neuronal Signaling
- Nuclear Receptors and Signaling
- Neuropeptides and Animal Physiology
- Pluripotent Stem Cells Research
- Pain Mechanisms and Treatments
- Sleep and Wakefulness Research
- Chromatin Remodeling and Cancer
- Autism Spectrum Disorder Research
- Tryptophan and brain disorders
- Mitochondrial Function and Pathology
University of Pennsylvania
2017-2024
Children's Hospital of Philadelphia
2017-2024
Lund University
2022
Ben-Gurion University of the Negev
2022
Soroka Medical Center
2022
Universitair Ziekenhuis Leuven
2022
KU Leuven
2022
The University of Texas Southwestern Medical Center
2012-2021
Southwestern Medical Center
2008-2020
California University of Pennsylvania
2020
Recent studies suggest that stress-induced atrophy and loss of hippocampal neurons may contribute to the pathophysiology depression. The aim this study was investigate effect antidepressants on neurogenesis in adult rat, using thymidine analog bromodeoxyuridine (BrdU) as a marker for dividing cells. Our demonstrate chronic antidepressant treatment significantly increases number BrdU-labeled cells dentate gyrus hilus hippocampus. Administration several different classes antidepressant, but...
Recent work implicates regulation of neurogenesis as a form plasticity in the adult rat hippocampus. Given known effects opiates such morphine and heroin on hippocampal function, we examined opiate this brain region. Chronic administration decreased by 42% granule cell layer. A similar effect was seen rats after chronic self-administration heroin. Opiate not mediated changes circulating levels glucocorticoids, because were that received adrenalectomy corticosterone replacement. These...
The transcription factor cAMP response element (CRE)-binding protein (CREB) has been shown to regulate neural plasticity. Drugs of abuse activate CREB in the nucleus accumbens, an important part brain's reward pathways, and local manipulations activity have affect cocaine reward, suggesting active role adaptive processes that follow exposure drugs abuse. Using CRE-LacZ reporter mice, we show not only rewarding stimuli such as morphine, but also aversive stress, CRE-mediated accumbens shell....
Understanding the fate of adult-generated neurons and mechanisms that influence them requires consistent labeling tracking large numbers stem cells. We generated a nestin-CreER T2 /R26R-yellow fluorescent protein (YFP) mouse to inducibly label nestin-expressing cells their progeny in adult subventricular zone (SVZ) subgranular (SGZ). Several findings show estrogen ligand tamoxifen (TAM) specifically induced recombination /R26R-YFP mice: 97% SGZ stem-like (GFAP/Sox2 with radial glial...
Acute seizures after a severe brain insult can often lead to epilepsy and cognitive impairment. Aberrant hippocampal neurogenesis follows the but role of adult-generated neurons in development chronic or associated deficits remains be determined. Here we show that ablation adult before pilocarpine-induced acute mice leads reduction seizure frequency. We also normalizes epilepsy-associated deficits. Remarkably, effect ablating is long lasting as it suppresses frequency for nearly 1 year....
Abnormal subgranular zone (SGZ) neurogenesis is proposed to contribute Alzheimer's disease (AD)-related decreases in hippocampal function. Our goal was examine the PDAPP mouse, a model of AD with age-dependent accumulation amyloid-β42 (Aβ42)-containing plaques that well studied regard therapies. A secondary determine whether altered mouse associated abnormal maturation or number mature cells. tertiary provide insight into why appears be increased post-mortem tissue and decreased most models....
The long-term response to chronic stress is variable, with some individuals developing maladaptive functioning, although other “resilient” do not. Stress reduces neurogenesis in the dentate gyrus subgranular zone (SGZ), but it unknown if stress-induced changes contribute individual vulnerability. Using a social defeat model, we explored whether susceptibility avoidance was related SGZ proliferation and neurogenesis. Immediately after defeat, stress-exposed mice (irrespective of they...
Notch1 regulates neural stem cell (NSC) number during development, but its role in adult neurogenesis is unclear. We generated nestin-CreER T2 /R26R-YFP/Notch1 loxP/loxP [Notch1inducible knock-out (iKO)] mice to allow tamoxifen (TAM)-inducible elimination of and concomitant expression yellow fluorescent protein (YFP) nestin-expressing Type-1 NSCs their progeny the hippocampal subgranular zone (SGZ). Consistent with previous research, YFP+ cells all stages were evident (SGZ) wild-type (WT)...
The neurobiological underpinnings of mood and anxiety disorders have been linked to the nucleus accumbens (NAc), a region important in processing rewarding emotional salience stimuli. Using chronic social defeat stress, an animal model disorders, we investigated whether alterations synaptic plasticity are responsible for long-lasting behavioral symptoms induced by this form stress. We hypothesized that stress alters strength or connectivity medium spiny neurons (MSNs) NAc induce avoidance....
Ascl1 (Mash1) is a bHLH transcription factor essential for neural differentiation during embryogenesis but its role in adult neurogenesis less clear. Here we show that the brain dynamically expressed dentate gyrus subgranular zone (SGZ) and more rostral subventricular (SVZ). Specifically, find levels low SGZ Type-1 cells SVZ B increasing as transition to intermediate progenitor stages. In vivo genetic lineage tracing with tamoxifen (TAM) inducible Ascl1CreERT2 knock-in mouse strain shows...
The lateral hypothalamus (LH) is implicated in the behavioral actions of drugs abuse, but cellular and molecular basis this role unclear. Recent identification neuropeptides localized LH neurons has allowed for more specific studies function. LH-specific peptide orexin (hypocretin) been shown to be important arousal sleep regulation. However, cells project broadly throughout brain such that may influence other behaviors as well. In study, we show neurons, not nearby expressing...
Drugs of abuse dynamically regulate adult neurogenesis, which appears important for some types learning and memory. Interestingly, a major site the hippocampus, is in formation drug–context associations mediation drug-taking drug-seeking behaviors animal models addiction. Correlative evidence suggests an inverse relationship between hippocampal neurogenesis or behaviors, but lack causative link has made adult-generated neurons addiction unclear. We used rat intravenous cocaine...