A5102851635- Epigenetics and DNA Methylation
- Wood and Agarwood Research
- Genetics and Neurodevelopmental Disorders
- Microbial Metabolism and Applications
- Cultural Heritage Materials Analysis
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Genomics and Chromatin Dynamics
- Cannabis and Cannabinoid Research
- Histone Deacetylase Inhibitors Research
- Autism Spectrum Disorder Research
- Receptor Mechanisms and Signaling
- Stress Responses and Cortisol
- Prenatal Substance Exposure Effects
- Pain Mechanisms and Treatments
- Neuroendocrine regulation and behavior
- Tryptophan and brain disorders
- Identification and Quantification in Food
- Plant biochemistry and biosynthesis
- Ion channel regulation and function
- Coleoptera Taxonomy and Distribution
- MicroRNA in disease regulation
- Viral gastroenteritis research and epidemiology
- Protein Degradation and Inhibitors
- Botulinum Toxin and Related Neurological Disorders
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2024
State Administration of Traditional Chinese Medicine of the People's Republic of China
2018-2024
State Ethnic Affairs Commission
2022
Florida State University
2016-2022
Peking Union Medical College Hospital
2022
Allen Institute for Brain Science
2011-2020
Icahn School of Medicine at Mount Sinai
2010-2020
Shandong Agricultural University
2020
Institute of Medicinal Plant Development
2016-2018
Chinese Academy of Fishery Sciences
2017
ChIP-seq is increasingly being used for genome-wide profiling of histone modification marks. It particular importance to compare data two different conditions, such as disease vs. control, and identify regions that show differences in ChIP enrichment. We have developed a powerful easy use program, called diffReps, detect those differential sites from data, with or without biological replicates. In addition, we useful tools analysis the diffReps package: one annotation other finding chromatin...
Depression and anxiety disorders are more prevalent in females, but the majority of research animal models, first step finding new treatments, has focused predominantly on males. Here we report that exposure to subchronic variable stress (SCVS) induces depression-associated behaviors female mice, whereas males resilient as they do not develop these behavioral abnormalities. In concert with different responses, transcriptional analysis nucleus accumbens (NAc), a major brain reward region, by...
We have evaluated the possibility that action of voluntary exercise on regulation brain-derived neurotrophic factor (BDNF), a molecule important for rat hippocampal learning, could involve mechanisms epigenetic regulation. focused studies Bdnf promoter IV, as this region is highly responsive to neuronal activity. found stimulates DNA demethylation in and elevates levels activated methyl-CpG-binding protein 2, well BDNF mRNA hippocampus. Chromatin immunoprecipitation assay showed increases...
Decreased medial prefrontal cortex (mPFC) neuronal activity is associated with social defeat-induced depression- and anxiety-like behaviors in mice. However, the molecular mechanisms underlying decreased mPFC its prodepressant role remain unknown. We show here that induction of transcription factor ΔFosB mPFC, specifically prelimbic (PrL) area, mediates susceptibility to stress. PrL occurred selectively susceptible mice after chronic defeat stress, overexpression this region, but not nearby...
Abstract Background Increasing evidence supports a role for altered gene expression in mediating the lasting effects of cocaine on brain, and recent work has demonstrated involvement chromatin modifications these alterations. However, all such studies to date have been restricted by their reliance microarray technologies that intrinsic limitations. Results We use next generation sequencing methods, RNA-seq ChIP-seq RNA polymerase II several histone methylation marks, obtain more complete...
Abstract Nerve injury induces changes in gene transcription dorsal root ganglion (DRG) neurons, which may contribute to nerve injury-induced neuropathic pain. DNA methylation represses expression. Here, we report that peripheral increases expression of the methyltransferase DNMT3a injured DRG neurons via activation factor octamer 1. Blocking this increase prevents voltage-dependent potassium (Kv) channel subunit Kcna2 promoter region and rescues attenuates Conversely, absence injury,...
Synapsins are a family of neuron-specific synaptic vesicle-associated phosphoproteins that have been implicated in synaptogenesis and the modulation neurotransmitter release. In mammals, distinct genes for synapsins I II identified, each which gives rise to two alternatively spliced isoforms. We now cloned characterized third member synapsin gene family, III, from human DNA. Synapsin III at least one protein isoform, designated IIIa, several mammalian species. IIIa is associated with...
Repeated cocaine exposure induces persistent alterations in genome-wide transcriptional regulatory networks, chromatin remodeling activity and, ultimately, gene expression profiles the brain's reward circuitry. Virtually all previous investigations have centered on drug-mediated effects occurring throughout active euchromatic regions of genome, with very little known concerning impact regulation and maintenance heterochromatin adult brain. Here, we report that dramatically dynamically alters...
The transcription factor ΔFosB and the brain-enriched calcium/calmodulin-dependent protein kinase II (CaMKIIα) are induced in nucleus accumbens (NAc) by chronic exposure to cocaine or other psychostimulant drugs of abuse, which two proteins mediate sensitized drug responses. Although CaMKIIα both regulate AMPA glutamate receptor expression function NAc, dendritic spine formation on NAc medium spiny neurons (MSNs), locomotor sensitization cocaine, no direct link between these molecules has...
Regulating Opioid Responses Different drugs of abuse are thought to highjack similar reward systems in the brain using common mechanisms. However, Koo et al. (p. 124 ) now observe that some neural mechanisms regulate opiate can be both different and even opposite those by stimulant drugs. While knockdown brain-derived neurotrophic factor (BDNF) ventral tegmental area mice antagonized response cocaine, same manipulation strengthened potential opiates increase dopamine neuron excitability....
Sirtuins (SIRTs), class III histone deacetylases, are well characterized for their control of cellular physiology in peripheral tissues, but influence brain under normal and pathological conditions remains poorly understood. Here, we establish an essential role SIRT1 SIRT2 regulating behavioral responses to cocaine morphine through actions the nucleus accumbens (NAc), a key reward region. We show that chronic administration increases expression mouse NAc, while induces alone, with no...
Previous studies have shown that chronic cocaine administration induces SIRT1, a Class III histone deacetylase, in the nucleus accumbens (NAc), key brain reward region, and such induction influences gene regulation place conditioning effects of cocaine. To determine mechanisms by which SIRT1 mediates cocaine-induced plasticity NAc, we used chromatin immunoprecipitation followed massively parallel sequencing (ChIP-seq), 1 d after 7 daily (20 mg/kg) or saline injections, to map binding...
Epigenetic mechanisms, such as histone modifications, regulate responsiveness to drugs of abuse, cocaine, but relatively little is known about the regulation addictive-like behaviors by DNA methylation. To investigate influence methylation on locomotor-activating effects cocaine and drug-seeking behavior, rats receiving methyl supplementation via chronic l-methionine (MET) underwent either a sensitization regimen intermittent injections or intravenous self-administration followed cue-induced...
Opioids are the gold standard for pharmacological treatment of neuropathic pain, but their analgesic effects unsatisfactory in part due to nerve injury-induced downregulation opioid receptors dorsal root ganglia (DRG) neurons. How injury drives such remains elusive. DNA methyltransferase (DNMT)-triggered methylation represses gene expression. We show here that blocking increase DRG DNMT3a (a de novo DNMT) rescued expression Oprm1 and Oprk1 mRNAs respective encoding mu-opioid receptor (MOR)...
To identify novel targets for neuropathic pain, 3097 mouse knockout lines were tested in acute and persistent pain behavior assays. One of the from this screen, which contained a null allele adapter protein-2 associated kinase 1 (AAK1) gene, had normal response assays (hot plate, phase I formalin), but markedly reduced to II formalin. AAK1 mice also failed develop tactile allodynia following Chung procedure spinal nerve ligation (SNL). Based on these findings, potent, small-molecule...