A5014846775- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Pulmonary Hypertension Research and Treatments
- Congenital heart defects research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- COVID-19 epidemiological studies
- Tissue Engineering and Regenerative Medicine
- Immune cells in cancer
- Cancer-related gene regulation
- Pancreatic function and diabetes
- Cancer Immunotherapy and Biomarkers
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Autophagy in Disease and Therapy
- Diabetes Management and Research
- Diabetes and associated disorders
- Calcium signaling and nucleotide metabolism
- Cancer Research and Treatments
- COVID-19 and Mental Health
University of Macau
2020-2025
Zhuhai Institute of Advanced Technology
2024
City University of Macau
2023
Stanford University
2015-2021
Cardiovascular Institute of the South
2015-2020
Palo Alto University
2020
StemCells (United States)
2020
Allen Institute for Brain Science
2013-2017
Icahn School of Medicine at Mount Sinai
2013-2017
Max Delbrück Center
2017
Understanding the relationship between millions of functional DNA elements and their protein regulators, how they work in conjunction to manifest diverse phenotypes, is key advancing our understanding mammalian genome. Next-generation sequencing technology now used widely probe these protein-DNA interactions profile gene expression at a genome-wide scale. As cost continues fall, interpretation ever increasing amount data generated represents considerable challenge. We have developed ngs.plot...
ChIP-seq is increasingly being used for genome-wide profiling of histone modification marks. It particular importance to compare data two different conditions, such as disease vs. control, and identify regions that show differences in ChIP enrichment. We have developed a powerful easy use program, called diffReps, detect those differential sites from data, with or without biological replicates. In addition, we useful tools analysis the diffReps package: one annotation other finding chromatin...
In familial pulmonary arterial hypertension (FPAH), the autosomal dominant disease-causing BMPR2 mutation is only 20% penetrant, suggesting that genetic variation provides modifiers alleviate disease. Here, we used comparison of induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) from three families with unaffected carriers (UMCs), FPAH patients, and gender-matched controls to investigate this variation. Our analysis identified features UMC iPSC-ECs related signaling or...
Abstract Background Increasing evidence supports a role for altered gene expression in mediating the lasting effects of cocaine on brain, and recent work has demonstrated involvement chromatin modifications these alterations. However, all such studies to date have been restricted by their reliance microarray technologies that intrinsic limitations. Results We use next generation sequencing methods, RNA-seq ChIP-seq RNA polymerase II several histone methylation marks, obtain more complete...
Abstract Background MicroRNA (miRNA) play an important role in gene expression regulation. At present, the number of annotated miRNA continues to grow rapidly, part due advances high-throughput sequencing techniques. Here, we use deep characterize a population small RNA expressed human and rhesus macaques brain cortex. Results Based on total more than 150 million sequence reads identify 197 putative novel miRNA, humans macaques, that are highly conserved among mammals. These have significant...
Sirtuins (SIRTs), class III histone deacetylases, are well characterized for their control of cellular physiology in peripheral tissues, but influence brain under normal and pathological conditions remains poorly understood. Here, we establish an essential role SIRT1 SIRT2 regulating behavioral responses to cocaine morphine through actions the nucleus accumbens (NAc), a key reward region. We show that chronic administration increases expression mouse NAc, while induces alone, with no...
Previous studies have shown that chronic cocaine administration induces SIRT1, a Class III histone deacetylase, in the nucleus accumbens (NAc), key brain reward region, and such induction influences gene regulation place conditioning effects of cocaine. To determine mechanisms by which SIRT1 mediates cocaine-induced plasticity NAc, we used chromatin immunoprecipitation followed massively parallel sequencing (ChIP-seq), 1 d after 7 daily (20 mg/kg) or saline injections, to map binding...
With extended stays aboard the International Space Station (ISS) becoming commonplace, there is a need to better understand effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) study cell-level function and gene expression. The hiPSC-CMs were cultured ISS for 5.5 weeks their expression, structure, functions compared with ground control hiPSC-CMs. Exposure caused alterations in hiPSC-CM calcium handling. RNA-sequencing...
Background: Metabolic alterations provide substrates that influence chromatin structure to regulate gene expression determines cell function in health and disease. Heightened proliferation of smooth muscle cells (SMC) leading the formation a neointima is feature pulmonary arterial hypertension (PAH) systemic vascular Increased glycolysis linked proliferative phenotype these SMC. Methods: RNA sequencing was applied SMC (PASMC) from PAH patients with without BMPR2 (bone morphogenetic receptor...
Parkinson's disease (PD) is a neurodegenerative disorder characterized by the degeneration of dopamine (DA) neurons in midbrain substantia nigra pars compacta (SNpc). While existing therapeutic strategies can alleviate PD symptoms, they cannot inhibit DA neuron loss. Herein, tailor-made human serum albumin (HSA)-based selenium nanosystem (HSA/Se nanoparticles, HSA/Se NPs) to treat that overcome intestinal epithelial barrier (IEB) and blood-brain (BBB) described. HSA, transporter for drug...
Long noncoding RNAs known as roX (RNA on the X) are crucial for male development in Drosophila, their loss leads to lethality from late larval stages. While recognized role sex-chromosome dosage compensation, ensuring balanced expression of X-linked genes both sexes, potential influence autosomal gene regulation remains unexplored. Here, using an integrative multi-omics approach, we show that not only govern X chromosome but also target autosomes lack male-specific lethal (MSL) complex...
Abstract PTEN-induced kinase-1 (PINK1) is a crucial player in selective clearance of damaged mitochondria via the autophagy-lysosome pathway, process termed mitophagy. Previous studies on PINK1 mainly focused its post-translational modifications, while transcriptional regulation much less understood. Herein, we reported novel mechanism control transcription by SMAD Family Member 3 (SMAD3), an essential component transforming growth factor beta (TGFβ)-SMAD signaling pathway. First, observed...
Rationale: Idiopathic or heritable pulmonary arterial hypertension is characterized by loss and obliteration of lung vasculature. Endothelial cell dysfunction pivotal to the pathophysiology, but different causal mechanisms may reflect a need for patient-tailored therapies.Objectives: cells differentiated from induced pluripotent stem were compared with endothelial same patients idiopathic hypertension, determine whether they shared functional abnormalities altered gene expression patterns...