A5100373912- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Congenital heart defects research
- 3D Printing in Biomedical Research
- Plant biochemistry and biosynthesis
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Renal and related cancers
- Heart Rate Variability and Autonomic Control
- Circular RNAs in diseases
- Sesquiterpenes and Asteraceae Studies
- RNA Interference and Gene Delivery
- Liver physiology and pathology
- Natural product bioactivities and synthesis
- RNA modifications and cancer
- RNA Research and Splicing
- Antioxidant Activity and Oxidative Stress
- Cancer, Hypoxia, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Cancer-related molecular mechanisms research
- Congenital Heart Disease Studies
- Epigenetics and DNA Methylation
- Cardiac electrophysiology and arrhythmias
- Pancreatitis Pathology and Treatment
- Cardiac Fibrosis and Remodeling
Cardiovascular Institute of the South
2016-2025
Nantong University
2014-2025
Central South University
2017-2025
Second Xiangya Hospital of Central South University
2018-2025
Chengdu University of Traditional Chinese Medicine
2022-2025
Chengdu University
2025
Stanford University
2015-2024
Medical College of Wisconsin
2024
Land Consolidation and Rehabilitation Center
2024
Nanjing Agricultural University
2024
Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation technically challenging strategies such as nuclear transfer used reprogramming limit their clinical applications. Here, we show that pluripotent generated mouse at a frequency up to 0.2% using combination of seven small-molecule compounds. The chemically resemble embryonic terms gene expression...
ABSTRACT The advent of human induced pluripotent stem cells (iPSCs) presents unprecedented opportunities to model diseases. Differentiated derived from iPSCs in two-dimensional (2D) monolayers have proven be a relatively simple tool for exploring disease pathogenesis and underlying mechanisms. In this Spotlight article, we discuss the progress limitations current 2D iPSC disease-modeling platform, as well recent advancements development models that mimic vivo tissues organs at...
Endothelial cells (ECs) display considerable functional heterogeneity depending on the vessel and tissue in which they are located. Whereas these differences presumably imprinted transcriptome, pathways networks that sustain EC have not been fully delineated.To investigate transcriptomic basis of specificity, we analyzed single-cell RNA sequencing data from tissue-specific mouse ECs generated by Tabula Muris consortium. We used a number bioinformatics tools to uncover markers sources data.We...
Erythropoietin (Epo) is required for the production of mature red blood cells. The requirement Epo and its receptor (EpoR) normal heart development response vascular endothelium cells neural origin to provide evidence that function as a growth factor or cytokine protect from apoptosis extends beyond hematopoietic lineage. We now report EpoR expressed on myoblasts can mediate biological these treatment with Epo. Primary murine satellite myoblast C2C12 cells, both which express endogenous...
Background The von Hippel–Lindau tumour suppressor protein–hypoxia-inducible factor (VHL–HIF) pathway has attracted widespread medical interest as a transcriptional system controlling cellular responses to hypoxia, yet insights into its role in systemic human physiology remain limited. Chuvash polycythaemia recently been defined new form of VHL-associated disease, distinct from the classical inherited cancer syndrome, which germline homozygosity for hypomorphic VHL allele causes generalised...
Establishment of efficient genome editing tools is essential for fundamental research, genetic engineering, and gene therapy. Successful construction application transcription activator-like effector nucleases (TALENs) in several organisms herald an exciting new era editing. We describe the production two active TALENs their successful targeted mutagenesis silkworm, Bombyx mori, whose manipulation methods are parallel to those Drosophila other insects. will also show that simultaneous...
Long noncoding RNAs (lncRNAs) are emerging as important regulators in various biological processes. However, to date, no systematic characterization of lncRNAs has been reported the silkworm Bombyx mori. In present study, we generated eighteen RNA-seq datasets with relatively high depth. Using an in-house designed lncRNA identification pipeline, 11,810 were identified for 5,556 loci. Among these lncRNAs, 474 transcripts intronic (ilncRNAs), 6,250 intergenic (lincRNAs), and 5,086 natural...
With extended stays aboard the International Space Station (ISS) becoming commonplace, there is a need to better understand effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) study cell-level function and gene expression. The hiPSC-CMs were cultured ISS for 5.5 weeks their expression, structure, functions compared with ground control hiPSC-CMs. Exposure caused alterations in hiPSC-CM calcium handling. RNA-sequencing...
Patient-specific iPSCs model endothelial dysfunction in lamin A and C–related dilated cardiomyopathy identify lovastatin as a therapy.
AT-rich interaction domain protein 1A (ARID1A), a SWI/SNF chromatin remodeling complex subunit, is frequently mutated across various cancer entities. Loss of ARID1A leads to DNA repair defects. Here, we show that plays epigenetic roles promote both double-strand breaks (DSBs) pathways, non-homologous end-joining (NHEJ) and homologous recombination (HR). accumulated at DSBs after damage regulates loops formation by recruiting RAD21 CTCF DSBs. Simultaneously, facilitates transcription...
Neonatal mouse hearts have transient renewal capacity, which is lost in juvenile and adult stages. In neonatal hearts, myocardial infarction (MI) causes an initial loss of cardiomyocytes. However, it unclear type regulated cell death (RCD) occurs stressed the current studies, we induced MI showed that ischemic cardiomyocytes primarily undergo ferroptosis, a non-apoptotic iron-dependent form RCD. We demonstrated cardiac fibroblasts (CFs) protect from ferroptosis through paracrine effects...
Spinal muscular atrophy (SMA) is a severe neurodegenerative disorder caused by deficiency of survival motor neuron (SMN). While significant progress has been made in SMA therapy rescuing SMN expression, limited knowledge about downstream genes hindered the development alternative therapies. Here, we conducted whole-transcriptome sequencing spinal cord, heart, and liver tissues mouse model at early postnatal ages to explore critical coding non-coding RNAs (ncRNAs). A large number...
BACKGROUND: Gene mutations are responsible for a sizeable proportion of cases heart failure. However, the number patients with any specific mutation is small. Repositioning existing US Food and Drug Administration–approved compounds to target promising approach efficient identification new therapies these patients. METHODS: The National Institutes Health Library Integrated Network-Based Cellular Signatures database was interrogated identify that demonstrated ability reverse transcriptional...