A5030635499- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- Mesenchymal stem cell research
- Spine and Intervertebral Disc Pathology
- Chemotherapy-induced cardiotoxicity and mitigation
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Fibrosis and Remodeling
- Congenital heart defects research
- Spaceflight effects on biology
- Bone Tissue Engineering Materials
- Diet, Metabolism, and Disease
- Neuroscience and Neural Engineering
- Radiation Therapy and Dosimetry
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Tendon Structure and Treatment
- CRISPR and Genetic Engineering
- Transplantation: Methods and Outcomes
- Cannabis and Cannabinoid Research
- Effects of Radiation Exposure
- Electrochemical sensors and biosensors
- Diagnosis and Treatment of Venous Diseases
Stanford University
2017-2025
Cardiovascular Institute of the South
2017-2025
California Institute for Regenerative Medicine
2021-2024
Palo Alto University
2019-2024
Ollscoil na Gaillimhe – University of Galway
2012-2021
Science Foundation Ireland
2018-2020
National University of Ireland
2017
Eastman Dental Hospital
2013
Cell therapy of the degenerated intervertebral disc is limited by lack appropriate cell sources, thus new strategies for differentiation stem cells towards a nucleus pulposus (NP)-like phenotype need investigation. In current study, it hypothesized that spherical niche-like structures composed type II collagen and hyaluronan (HA) mimic NP microenvironment promote adipose-derived (ADSCs) an NP-like phenotype. ADSCs are embedded in microgels different concentrations II/HA. Cells' response to...
Excessive iron accumulation in the heart causes overload cardiomyopathy (IOC), which initially presents as diastolic dysfunction and arrhythmia but progresses to systolic end-stage failure when left untreated. However, mechanisms of iron-related cardiac injury how accumulates human cardiomyocytes are not well understood. Herein, using induced pluripotent stem cell-derived (iPSC-CMs), we model IOC screen for drugs rescue phenotypes. Human iPSC-CMs under excess exposure recapitulate...
Significance An optimal 3D cell-delivery platform is key for cytoprotection and prolonged cell function in vivo sustained delivery of therapeutic factors as “cell factories.” However, little known about stem-cell phenotype behavior resulting from vitro preconditioning engineered extracellular matrices. We demonstrate a close relationship between macromolecular concentration mesenchymal stem that drives morphological changes, modulation integrin expression, matrix rigidity after 96 h...
Abstract Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, TGF-β1 leads to progressive fibrosis. Decorin (DCN) interleukin 10 (IL-10) antagonise pathological collagen exerting a neutralising effect via downregulation TGF-β1. Herein, we report that the delivery DCN IL-10 transgenes from hydrogel system supresses constitutive expression range pro-fibrotic extracellular genes.
Long COVID, or post-acute sequelae of SARS-CoV-2 infection, leads to vascular dysfunction, which contributes the chronic multi-organ damage often seen in affected patients. a global health concern is associated with increased thrombotic risk, also known as COVID-19-associated coagulopathy (CAC). Here, we derived an induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells (PBMCs) long COVID patient. This iPSC showed normal morphology, maintained pluripotency, had...
Cells directly probe and respond to the physicomechanical properties of their extracellular environment, a dynamic process which has been shown play key role in regulating both cellular adhesive processes differential function. Recent studies indicate that stem cells show lineage-specific differentiation when cultured on substrates approximating stiffness profiles specific tissues. Although tissues are associated with range Young's modulus values for bulk rigidity, at subcellular level,...
Critical limb ischemia (CLI) is characterized by the impairment of microcirculation, necrosis and inflammation muscular tissue. Although role glycans in mediating has been reported, changes glycosylation following muscle remains poorly understood. Here, a murine CLI model was used to show increase high mannose, α-(2, 6)-sialic acid decrease hybrid bisected N-glycans as associated with ischemic environment. Using this model, efficacy an elastin-like recombinamers (ELR) hydrogel assessed. The...
Immunocompromised hind limb ischemia (HLI) murine models are essential for preclinical evaluation of human cell-based therapy or biomaterial-based interventions. These used to generate proof principle that the approach is effective and also regulatory data required translation clinic. However, surgical variations in creation HLI reported literature introduce variability pathological manifestation model, consequence affecting therapeutic endpoints. This study aims compare extent vascular...
Human pluripotent stem cells (PSCs), which are composed of embryonic (ESCs) and induced (iPSCs), provide an opportunity to advance cardiac cell therapy-based clinical trials. However, important hurdle that must be overcome is the risk teratoma formation after transplantation due proliferative capacity residual undifferentiated PSCs in differentiation batches. To tackle this problem, we propose use a minimal noncardiotoxic doxorubicin dose as purifying agent selectively target rapidly...