A5002167350- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Electrospun Nanofibers in Biomedical Applications
- Pain Mechanisms and Treatments
- Tissue Engineering and Regenerative Medicine
- Medical Imaging and Analysis
- Wound Healing and Treatments
- Shoulder Injury and Treatment
- Anesthesia and Pain Management
- Diatoms and Algae Research
- Proteoglycans and glycosaminoglycans research
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Hydrogels: synthesis, properties, applications
- Barrier Structure and Function Studies
- Osteoarthritis Treatment and Mechanisms
- Analytical Chemistry and Sensors
- Protease and Inhibitor Mechanisms
- Tendon Structure and Treatment
- Free Radicals and Antioxidants
- 3D Printing in Biomedical Research
- Immune Response and Inflammation
- Surgical Sutures and Adhesives
- Vitamin C and Antioxidants Research
National University of Malaysia
2020-2025
University Hospital Galway
2023-2025
Ollscoil na Gaillimhe – University of Galway
2015-2023
Science Foundation Ireland
2020
National University of Ireland
2019
Painful intervertebral disc degeneration is mediated by inflammation that modulates glycosylation and induces hyperinnervation sensory sensitization, which result in discogenic pain. Hyaluronic acid (HA) used as a therapeutic biomaterial can reduce pain, but the effects of HA therapy on pain associated with have not been previously determined. We describe novel rat model induced injury, validation phenotype morphine treatment. Using this model, we assessed efficacy hydrogel for alleviation...
Inflammation plays an important role in symptomatic intervertebral disc degeneration and is associated with the production of neurotrophins sensitizing innervation into disc. The use high molecular weight (HMw) hyaluronic acid (HA) hydrogels offers a potential therapeutic biomaterial for nucleus pulposus (NP) regeneration as it exerts anti-inflammatory effect provides microenvironment that more suitable NP. Therefore, was hypothesized cross-linked HMw HA modulate inflammatory receptor...
The active stages of intestinal inflammation and the pathogenesis ulcerative colitis are associated with superficial mucosal damage intermittent wounding that leads to epithelial barrier defects increased permeability. standard therapeutic interventions for have focused mainly on maintaining remission levels disease. Nonetheless, such treatment strategies (using anti-inflammatory, immunomodulatory agents) do not address colitis' root cause, especially dysregulated functions. Restoration...
Intervertebral disc (IVD) degeneration is one of the primary causes low back pain, causing disability; hence, there no regenerative nature current treatments. Hyaluronic acid (HA) was reported to facilitate tissue repair and alleviate pain. Herein, we determined therapeutic effect HA type II collagen (COLII) hydrogel for targeting pain in IVD degeneration. We implanted HA/COLII following surgically induced injury at coccygeal levels rat tail model assessed efficacy reducing behaviour by...
Significance An optimal 3D cell-delivery platform is key for cytoprotection and prolonged cell function in vivo sustained delivery of therapeutic factors as “cell factories.” However, little known about stem-cell phenotype behavior resulting from vitro preconditioning engineered extracellular matrices. We demonstrate a close relationship between macromolecular concentration mesenchymal stem that drives morphological changes, modulation integrin expression, matrix rigidity after 96 h...
Introduction: A regenerative strategy employing extracellular matrix (ECM)-based biomaterials and stem cells provide a better approach to mimicking the three-dimensional (3D) microenvironment of intervertebral disc for endogenous tissue regeneration. However, there is currently limited understanding regarding human Wharton Jelly derived-mesenchymal (hWJ-MSCs) towards nucleus pulposus (NP)-like cells. Our study focused on development 3D bioengineered hydrogel based predominant ECM native NP,...
In spine research, human degenerative discs are commonly procured from patients undergoing spinal surgery; however, the acquisition of healthy as age-matched controls remains highly restricted. This study establishes a protocol for dissecting body donors, aiming to harvest intact lumbar intervertebral (IVDs). A one-year prospective cadaveric dissection on through donation programme at public institution. Thirteen donors with no history diseases were identified. Consent was obtained family...
Study Design. An in vivo model to study the effect of an injectable hyaluronic acid (HA) hydrogel following puncture-induced lumbar disc injury rabbits. Objectives. The aim this was determine efficacy HA maintain height and tissue hydration, promote structural repair, attenuate inflammation innervation discs. Summary Background Data. Previously, we have demonstrated that alleviated inflammation, innervation, pain repair. Nevertheless, a weight-bearing animal not performed. Methods. We...
Intervertebral disc (IVD) degeneration causes low-back pain through compression, prolapse and herniation. Inflammation of the IVD subsequent produce altered glycosylation profiles in several animal models injury ageing, although function this pattern a human is unknown. Altered N-glycome, specifically sialylated fucosylated N-glycosylation motif expression, might play role inflammation disease progression. Healthy (foetal adolescent idiopathic scoliosis) degenerated (lumbar degeneration)...
Mesenchymal stem cells (MSCs) can improve chronic wound healing; however, recent studies suggest that the therapeutic effect of MSCs is mediated mainly through growth factors and cytokines secreted by these cells, referred to as MSC secretome. To overcome difficulties related translation cell therapy into clinical use such efficacy, safety cost, we propose a hydrogel loaded with secretome from recently established human adipose tissue mesenchymal line (HATMSC2) potential treatment for...
Abstract Therapeutic factors secreted by mesenchymal stem cells (MSCs) promote angiogenesis in vivo . However, delivery of MSCs the absence a cytoprotective environment offers limited efficacy due to low cell retention, poor graft survival and non-maintenance physiologically relevant dose growth at injury site. The on an extracellular matrix (ECM)-based platform alters behaviour including migration, proliferation paracrine activity, which are essential for angiogenesis. We demonstrate...
Immunomodulatory biomaterials have emerged as a promising approach to engineer wound healing. To achieve this task, the bioactivity of and an easy application are two key desirable characteristics. This work reports injectable gel system containing immune cells primed for By combining self-assembly type I collagen, cross-linked with trifunctional oligourethanes, silica particle entrapment, structured collagen network acts delivery vehicle macrophages. primes macrophages anti-inflammatory...
The extracellular matrix (ECM)-based biomaterials provide a platform to mimic the disc microenvironment in facilitating stem cell transplantation for tissue regeneration. However, little is known about vitro preconditioning human umbilical cord Wharton Jelly-derived mesenchymal cells (MSCs) on 3D hyaluronic acid (HA)/type II collagen (COLII) hydrogel nucleus pulposus (NP) phenotype and pain modulation. We developed tuneable HA/COLII by fabricating at 2 mg/ml COLII various weight ratios of...