A5101875809- RNA Research and Splicing
- RNA modifications and cancer
- Neurogenetic and Muscular Disorders Research
- Urological Disorders and Treatments
- Urologic and reproductive health conditions
- Urinary and Genital Oncology Studies
- Crystallization and Solubility Studies
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- X-ray Diffraction in Crystallography
- Advancements in Battery Materials
- 14-3-3 protein interactions
- Macrophage Migration Inhibitory Factor
- RNA and protein synthesis mechanisms
- Muscle Physiology and Disorders
- Zebrafish Biomedical Research Applications
- Advanced Battery Materials and Technologies
- Pediatric Urology and Nephrology Studies
- Neuroscience and Music Perception
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Sexual Differentiation and Disorders
- Gut microbiota and health
- Congenital heart defects research
- MicroRNA in disease regulation
Nantong University
2020-2025
Nanjing Medical University
2025
Guangxi Medical University
2024
Xi'an Jiaotong University
2024
Henan University of Science and Technology
2024
Shenzhen Children's Hospital
2021-2023
Sir Run Run Shaw Hospital
2022
Zhejiang University
2020-2022
Pusan National University
2022
Children's Hospital of Chongqing Medical University
2021
Spinal muscular atrophy (SMA) is a neuromuscular disease caused by reduced expression of survival motor neuron (SMN), protein expressed in humans two paralogous genes, SMN1 and SMN2. These genes are nearly identical, except for 10 single-nucleotide differences 5-nucleotide insertion SMA subdivided into four main types, with type I being the most severe. SMN2 copy number key positive modifier disease, but it not always inversely correlated clinical severity. We previously reported c.859G > C...
Spinal muscular atrophy (SMA) is a severe neurodegenerative disorder caused by deficiency of survival motor neuron (SMN). While significant progress has been made in SMA therapy rescuing SMN expression, limited knowledge about downstream genes hindered the development alternative therapies. Here, we conducted whole-transcriptome sequencing spinal cord, heart, and liver tissues mouse model at early postnatal ages to explore critical coding non-coding RNAs (ncRNAs). A large number...
Abstract Skeletal muscular atrophy is a complex disease involving large number of gene expression regulatory networks and various biological processes. Despite extensive research on this topic, its underlying mechanisms remain elusive, effective therapeutic approaches are yet to be established. Recent studies have shown that epigenetics play an important role in regulating skeletal muscle atrophy, influencing the numerous genes associated with condition through addition or removal certain...
Background Inflammation may trigger skeletal muscle atrophy induced by cancer cachexia. As a pro-inflammatory factor, interleukin-6 cause atrophy, but the underlying molecular mechanisms have not been explored. Methods In this experimental study, we used adult male ICR mice, weighing 25 ± 2 g, and continuous infusion of into tibialis anterior to construct model (experimental group). A control group received saline infusion. RNA-sequencing was analyze differentially expressed genes in tissue...
Autism spectrum disorder (ASD) is a prevalent neurodevelopmental with complex etiology. Recent evidence suggests that dopamine plays crucial role in neural development. However, it remains unclear whether and how disrupted dopaminergic signaling during development contributes to ASD. In this study, human brain RNA-seq transcriptome analysis revealed significant correlation between changes pathways developmental ASD patients. the zebrafish model, led circuit abnormalities behavior reminiscent...
LCZ696 is a novel treatment for patients suffering from heart failure that combines the two active pharmaceutical ingredients sacubitril and valsartan in single chemical compound. While an established drug substance, new manufacturing process suitable large-scale commercial production had to be developed sacubitril. The use of chemocatalysis, biocatalysis, flow chemistry as state-of-the-art technologies allowed efficiently build up structure achieve defined performance targets.
Spinal muscular atrophy (SMA) is the leading genetic cause of infant mortality, characterized by progressive degeneration spinal-cord motor neurons, to skeletal muscles. However, accumulating evidence indicates that it a multi-system disorder, particularly in its severe forms. Several studies delineated structural and functional cardiac abnormalities SMA patients mouse models, yet have been primarily attributed autonomic dysfunction. Here, we show model cardiomyocytes undergo G0/G1...
RNA-binding proteins (RBPs) bind to RNAs and are crucial for regulating RNA splicing, stability, translation, transport. Among these proteins, the CUGBP Elav-like family (CELF) is a highly conserved group posttranscriptional regulation by binding CUG repeats. Comprising CELF1–6, this exhibits diverse expression patterns functions. Dysregulation of CELF has been implicated in various neural disorders, encompassing both neurodegenerative neurodevelopmental conditions, such as Alzheimer's...
The PDCD1 gene encodes PD-1, an important immune checkpoint protein and key immunotherapy target to treat cancer. is alternatively spliced generate exon 3-skipped isoform PD-1Δ3 that has been suggested play antagonistic role but the mechanism underlying alternative splicing of never explored. Here using a minigene system, we analysed pattern in multiple cell lines confirmed 3 skipping as main event. Using deletion analysis 3, mapped two enhancers exon: ESE3a ESE3b. mutagenesis, RNA-affinity...
Although spinal muscular atrophy (SMA) is a motor neuron disease caused by the loss of survival (SMN) proteins, there growing evidence that non-neuronal cells play important roles in SMA pathogenesis. However, transcriptome alterations occurring at single-cell level cord remain unknown, preventing us from fully comprehending role specific cells. Here, we performed RNA sequencing severe mouse model, and identified ten cell types as well their differentially expressed genes. Using CellChat,...
The pMN domain is a restricted in the ventral spinal cord, defined by expression of olig2 gene. Though it known that progenitor cells can sequentially generate motor neurons and oligodendrocytes, lineages these progenitors are controversial how their progeny generated not well understood. Using single-cell RNA sequencing, here, we identified previously unknown heterogeneity among with distinct fates molecular signatures zebrafish. Notably, characterized two neuron using bioinformatic...
Abstract Background Denervation-induced muscle atrophy is complex disease involving multiple biological processes with unknown mechanisms. N6-methyladenosine (m6A) participates in skeletal physiology by regulating levels of RNA metabolism, but its impact on denervation-induced still unclear. Here, we aimed to explore the changes, functions, and molecular mechanisms m6A methylation during atrophy. Methods During atrophy, immunoprecipitation sequencing (MeRIP-seq) as well enzyme-linked...
Abstract Alternative splicing (AS) presents a key posttranscriptional regulatory mechanism associated with numerous physiological processes. However, little is known about its role in skeletal muscle atrophy. In this study, we used rat model of denervated atrophy and performed RNA‐sequencing to analyze transcriptome profiling tibialis anterior at multiple time points following denervation. We found that AS novel involving atrophy, which independent changes the transcript level....