A5101694841- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Cell death mechanisms and regulation
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and biological activity
- Muscle Physiology and Disorders
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Axon Guidance and Neuronal Signaling
- Synthesis of Organic Compounds
- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Computational Drug Discovery Methods
- Glycosylation and Glycoproteins Research
- vaccines and immunoinformatics approaches
- Chemical Synthesis and Analysis
- Multicomponent Synthesis of Heterocycles
- Tissue Engineering and Regenerative Medicine
- Chromatography in Natural Products
- Cancer-related molecular mechanisms research
- Receptor Mechanisms and Signaling
- Trace Elements in Health
- Protein Degradation and Inhibitors
Shanxi Medical University
2025
Third Affiliated Hospital of Zhengzhou University
2025
University of California, San Diego
2011-2025
Sichuan University
2020-2025
Chinese University of Hong Kong, Shenzhen
2021-2025
Tsinghua University
2013-2025
Institute of Precision Mechanics
2025
Chinese University of Hong Kong
2025
Beijing Proteome Research Center
2024
Anhui Medical University
2024
Prions are composed largely, if not entirely, of prion protein (PrPSc in the case scrapie). Although formation PrPSc from cellular (PrPC) is a post-translational process, no candidate chemical modification was identified, suggesting that conformational change features synthesis. To assess this possibility, we purified both PrPC and by using nondenaturing procedures determined secondary structure each. Fourier-transform infrared (FTIR) spectroscopy demonstrated has high alpha-helix content...
Bcl-2 and related proteins are key regulators of apoptosis or programmed cell death implicated in human disease including cancer. We recently showed that cell-permeable binding peptides could induce myeloid leukemia vitro suppress its growth severe combined immunodeficient mice. Here we report the discovery HA14-1, a small molecule (molecular weight = 409) nonpeptidic ligand surface pocket, by using computer screening strategy based on predicted structure protein. In studies demonstrated...
NOD1 and NOD2 are members of the NOD-like receptor (NLR) protein family that involved in sensing presence pathogens a component innate immune system. Upon activation by specific bacterial peptides derived from peptidoglycans, NODs interact via CARD-CARD interaction with receptor-interacting kinase RIP2, an inducer NF-kappaB activation. In this report, we show NOD signaling is dependent on XIAP, member inhibitor apoptosis (IAP) family. Cells deficient XIAP exhibit marked reduction induced...
Smac/DIABLO is a mitochondrial protein that released along with cytochrome c during apoptosis and promotes c-dependent caspase activation by neutralizing inhibitor of proteins (IAPs). We provide evidence functions at the levels both Apaf-1-caspase-9 apoptosome effector caspases. The N terminus absolutely required for its ability to interact baculovirus IAP repeat (BIR3) XIAP promote activation. However, it less critical BIR1/BIR2 activity Consistent function level caspases, expression...
Prion diseases are a group of neurodegenerative disorders in humans and animals that seem to result from conformational change the prion protein (PrP). Utilizing data obtained by circular dichroism infrared spectroscopy, computational studies predicted three-dimensional structure cellular form PrP (PrPc). A heuristic approach consisting prediction secondary structures an evaluation packing elements was used search for plausible tertiary structures. After series experimental theoretical...
Renal cell carcinoma (RCC) is one of the most frequently observed malignant tumours in urinary system and targeted drug resistance quite common RCC. Long noncoding RNA SNHG12 (lncRNA SNHG12) has emerged as a key molecule numerous human cancers, but its functions renal sunitinib remain unclear. In this study, we found was highly expressed RCC tissues sunitinib-resistant cells associated with poor clinical prognosis. promoted proliferation, migration, invasion via CDCA3 vitro. Mechanically,...
The molecular mechanisms underlying denervated skeletal muscle atrophy with concomitant mass loss have not been fully elucidated. Therefore, this study aimed to attain a deeper understanding of the as critical step developing targeted therapy and retarding mass.We employed microarray analysis reveal potential atrophy. We used in vitro vivo models explore roles interleukin 6 (IL-6), Janus kinase (JAK), signal transducers activators transcription 3 (STAT3) atrophy.In study, differentially...
Denervation induces skeletal muscle atrophy, accompanied by complex biochemical and physiological changes, with potentially devastating outcomes even an increased mortality. Currently, however, there remains a paucity of effective strategies to treat atrophy. Therefore, it is required understand the molecular mechanisms atrophy formulate new treatment strategies. In this study, we investigated transcriptional profile denervated after peripheral nerve injury in rats. The cDNA microarray...
Caspases play a major role in the transduction of apoptotic signal and execution apoptosis mammalian cells. Ectopic overexpression short prodomain caspases-3 -6 precursors cells does not induce apoptosis. This is due to their inability undergo autocatalytic processing/activation suggests that they depend on long caspases for activation. To investigate directly activity these two <i>in vivo</i>, we engineered constitutively active recombinant precursors. was achieved by making contiguous...
The pro-apoptotic members of the Bcl-2 family include initiator proteins that contain only BH3 domains and downstream effector multi-BH domain-containing proteins, including Bax Bak. In this report, we compared ability six human anti-apoptotic to suppress apoptosis induced by overexpression or Bak, correlating findings with protein interactions measured three different methods: co-immunoprecipitation, glutathione S-transferase pulldown, fluorescence polarization assays employing synthetic...
Although denervated muscle atrophy is common, the underlying molecular mechanism remains unelucidated. We have previously found that oxidative stress and inflammatory response may be early events trigger atrophy. Isoquercitrin a biologically active flavonoid with antioxidative anti-inflammatory properties. The present study investigated effect of isoquercitrin on its possible mechanisms. was effective in alleviating mass loss following denervation dose-dependent manner. demonstrated optimal...
Skeletal muscle atrophy is a common and debilitating condition that lacks an effective therapy. Oxidative stress inflammation are two main molecular mechanisms involved in atrophy. In the current study, we want to explore whether how salidroside, with antioxidant anti-inflammatory properties, protects against skeletal induced by denervation. First, oxidative inflammatory response were examined during myotube nutrition deprivation. The results demonstrated cultured myotubes suffered from...
Skeletal muscle atrophy involves and requires widespread changes in skeletal gene expression signaling pathway, resulting excessive loss of mass strength, which is associated with poor prognosis the decline life quality several diseases. However, treatment remains an unresolved challenge to this day. The aim present study was investigate influence pyrroloquinoline quinone (PQQ), a redox-active o-quinone found various foods mammalian tissues, on atrophy, explore underlying molecular...
Sjögren’s syndrome (SS) is a chronic systemic autoimmune disease that typically presents with lymphocyte, dendritic cell, and macrophage infiltration of exocrine gland ducts the formation ectopic germinal centers. The interactions lymphocyte homing receptors addressins chemokines their receptors, such as α4β7/MAdCAM-1, LFA-1/ICAM-1, CXCL13/CXCR5, CCL25/CCR9, CX3CL1/CX3CR1, play important roles in migration inflammatory cells to focal glands promotion center SS. A variety molecules have been...
Cytochrome c release is a central step in the apoptosis induced by many death stimuli. Bcl-2 plays critical role controlling this step. In study, we investigated upstream mechanism of cytochrome ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (HA14-1), recently discovered small molecule inhibitor Bcl-2. HA14-1 was found to induce from mitochondria intact cells but not isolated mitochondria. requires presence both and exogenous Ca2+. This suggests that...