A5041326706- Silk-based biomaterials and applications
- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Silkworms and Sericulture Research
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Utilization and Effects
- Insect and Arachnid Ecology and Behavior
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Plant biochemistry and biosynthesis
- Insect-Plant Interactions and Control
- Animal Genetics and Reproduction
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
Southwest University
2016-2025
Ministry of Agriculture and Rural Affairs
2022-2025
State Key Laboratory of Silkworm Genomic Biology
2015-2024
Sericultural Research Institute
2019-2024
Jilin Province Development and Reform Commission
2023
Chongqing University of Science and Technology
2020-2022
Novel (United States)
2019-2021
National Institute of Diabetes and Digestive and Kidney Diseases
2021
ProQuest (United States)
2021
State Council of the People's Republic of China
2019
We report a draft sequence for the genome of domesticated silkworm (Bombyx mori), covering 90.9% all known genes. Our estimated gene count is 18,510, which exceeds 13,379 genes reported Drosophila melanogaster. Comparative analyses to fruitfly, mosquito, spider, and butterfly reveal both similarities differences in content.
OrthoVenn is a powerful web platform for the comparison and analysis of whole-genome orthologous clusters. Here we present an updated version, OrthoVenn2, which provides new features that facilitate comparative clusters among up to 12 species. Additionally, this update offers improvements data visualization interpretation, including occurrence pattern table interrogating overlap each group queried Within table, functional annotations summaries disjunctions intersections between chosen...
The Taming of the Silkworm Silkworms, Bombyx mori , represent one few domesticated insects, having been over 10,000 years ago. Xia et al. (p. 433 published online 27 August) sequenced 29 domestic and 11 wild silkworm lines identified genes that were most likely to be selected during domestication. These those enhance silk production, reproduction, growth. Furthermore, silkworms probably only once from a large progenitor population, rather than on multiple occasions, as has observed for other animals.
Human utilization of the mulberry–silkworm interaction started at least 5,000 years ago and greatly influenced world history through Silk Road. Complementing silkworm genome sequence, here we describe a mulberry species Morus notabilis. In 330-Mb assembly, identify 128 Mb repetitive sequences 29,338 genes, 60.8% which are supported by transcriptome sequencing. Mulberry gene appear to evolve ~3 times faster than other Rosales, perhaps facilitating species' spread worldwide. The tree is among...
The tobacco cutworm, Spodoptera litura, is among the most widespread and destructive agricultural pests, feeding on over 100 crops throughout tropical subtropical Asia. By genome sequencing, physical mapping transcriptome analysis, we found that gene families encoding receptors for bitter or toxic substances detoxification enzymes, such as cytochrome P450, carboxylesterase glutathione-S-transferase, were massively expanded in this polyphagous species, enabling its extraordinary ability to...
We designed and constructed a genome-wide microarray with 22,987 70-mer oligonucleotides covering the presently known predicted genes in silkworm genome, surveyed gene expression multiple tissues on day 3 of fifth instar. Clusters tissue-prevalent tissue-specific that are differentially expressed different were identified, they reflect well major functions molecular level. The data presented this study provide new resource for annotating genome.
A wound dressing with excellent hydrophilicity, high porosity, good mechanical property, and antimicrobial activities holds great potential for healing. Sericin, a natural protein from silkworm cocoon, is particularly attractive the development of skin-repairing materials because it biocompatible biodegradable. Here, we reported green strategy in situ biomimetic synthesis silver nanoparticles (AgNPs) within sericin/poly(vinyl alcohol) hydrogel. AgNPs were synthesized by redox property...
The SilkDB is an open-access database for genome biology of the silkworm (Bombyx mori). Since draft sequence was completed and first released 5 years ago, we have collaborated with other groups to make much remarkable progress on research, such as completion a new high-quality assembly well construction genome-wide microarray survey gene expression profiles. To accommodate these genomic data house more comprehensive information, reconstructed web interfaces. In version (v2.0) SilkDB, updated...
Abstract Background Chemosensory systems play key roles in the survival and reproductive success of insects. Insect chemoreception is mediated by two large diverse gene superfamilies, chemoreceptors odorant binding proteins (OBPs). OBPs are believed to transport hydrophobic odorants from environment olfactory receptors. Results We identified a family OBP-like genes silkworm genome characterized their expression using oligonucleotide microarrays. A total forty-four OBP were annotated, number...
Establishment of efficient genome editing tools is essential for fundamental research, genetic engineering, and gene therapy. Successful construction application transcription activator-like effector nucleases (TALENs) in several organisms herald an exciting new era editing. We describe the production two active TALENs their successful targeted mutagenesis silkworm, Bombyx mori, whose manipulation methods are parallel to those Drosophila other insects. will also show that simultaneous...
Microsporidian Nosema bombycis has received much attention because the pébrine disease of domesticated silkworms results in great economic losses silkworm industry. So far, no effective treatment could be found for pébrine. Compared to other known parasites, N. can unusually parasitize a broad range hosts. To gain some insights into underlying genetic mechanism pathological ability and host expansion this parasite, comparative genomic approach is conducted. The genome two antheraeae (an...
CRISPR/Cas9, a bacterial adaptive immune system derived genome-editing technique, has become to be one of the most compelling topics in biotechnology. Bombyx mori is an economically important insect and model organism for studying lepidopteran arthropod biology. Here we reported highly efficient multiplex genome editing B. cell line heritable site-directed mutagenesis Bmku70, which required NHEJ pathway also related antigen diversity, telomere length maintenance subtelomeric gene silencing,...
Insect gustatory receptors are predicted to have a seven-transmembrane structure and distantly related insect olfactory receptors, which an inverted topology compared with G-protein coupled including mammalian receptors. In contrast, the of remains unknown. Except for few examples from Drosophila, specificity individual is also this study, total number identified in Bombyx mori was expanded 65 69. BmGr8, silkmoth receptor sugar subfamily, expressed cells. Membrane studies on BmGr8 indicate...
Abstract Fundamental and applied studies of silkworms have entered the functional genomics era. Here, we report a multi-gene expression system (MGES) based on 2A self-cleaving peptide (2A), which regulates simultaneous cleavage multiple gene targets in silk gland transgenic silkworms. First, glycine-serine-glycine spacer (GSG) was found to significantly improve efficiency 2A. Then, six types 2As with GSG analyzed. The shortest porcine teschovirus-1 (P2A-GSG) exhibited highest all insect cell...
Autophagy is finely regulated at multiple levels and plays crucial roles in development disease. In the fat body of silkworm, Bombyx mori, autophagy occurs Atg gene expression peaks during nonfeeding molting pupation stages when steroid hormone (20-hydroxyecdysone; 20E) high. Injection 20E into feeding larvae upregulated genes reduced TORC1 activity resulting induction body. Conversely, RNAi knockdown receptor partner (USP) or targeted overexpression a dominant negative mutant (EcR (DN) )...
Long noncoding RNAs (lncRNAs) are emerging as important regulators in various biological processes. However, to date, no systematic characterization of lncRNAs has been reported the silkworm Bombyx mori. In present study, we generated eighteen RNA-seq datasets with relatively high depth. Using an in-house designed lncRNA identification pipeline, 11,810 were identified for 5,556 loci. Among these lncRNAs, 474 transcripts intronic (ilncRNAs), 6,250 intergenic (lincRNAs), and 5,086 natural...