A5021348749- Bacterial Genetics and Biotechnology
- Animal Nutrition and Physiology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Garlic and Onion Studies
- Protein Structure and Dynamics
- Pharmacological Effects of Natural Compounds
- Enzyme Structure and Function
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Gut microbiota and health
- Ginger and Zingiberaceae research
- Melanoma and MAPK Pathways
- Alcohol Consumption and Health Effects
- Invertebrate Immune Response Mechanisms
- Flavonoids in Medical Research
- Escherichia coli research studies
- Genetic Mapping and Diversity in Plants and Animals
- Biological Activity of Diterpenoids and Biflavonoids
- Virus-based gene therapy research
- Medicinal plant effects and applications
- Mangiferin and Mango Extracts
- Metabolism and Genetic Disorders
- Dye analysis and toxicity
Hunan Agricultural University
2017-2025
Kagoshima University
2019-2024
State Key Laboratory of Oral Diseases
2024
Sichuan University
2012-2024
Waseda University
2022-2024
Graduate School USA
2020
South China Agricultural University
2011-2019
Honghe University
2016-2019
Huazhong Agricultural University
2018
The Ohio State University
2003-2017
Autophagy and apoptosis, which could be induced by common stimuli, play crucial roles in development disease. The functional relationship between autophagy apoptosis is complex, due to the dual effects of autophagy. In Bombyx Bm-12 cells, 20-hydroxyecdysone (20E) treatment or starvation-induced cell death, with preceding apoptosis. response 20E starvation, BmATG8 was rapidly cleaved conjugated PE form BmATG8-PE; subsequently, BmATG5 BmATG6 were into BmATG5-tN BmATG6-C, respectively....
Garlic (Allium sativum L.) contains prebiotic components, fructans, antibacterial compounds, and organosulfur compounds. The complex ingredients of garlic seem to impart a paradoxical result on the gut microbiome. In this study, we used mouse model clarify effects whole C57BL/6N male mice were fed with or without in normal diet (ND) high-fat (HFD) for 12 weeks. Supplementation attenuated HFD-enhanced ratio serum GPT/GOT (glutamic-pyruvic transaminase/glutamic-oxaloacetic transaminase),...
VTP might prevent WD-induced NAFLD by balancing fatty acid oxidation and lipogenesis, hepatic oxidative stress, the gut microbiome.
Ampelopsis grossedentata, also called vine tea, has been used as a traditional beverage in China for centuries. Vine tea contains rich polyphenols and shows benefit to human health, but the chemical antioxidant properties of from different locations remain unclear. This study aims investigate three major production areas including Guizhou, Hunan, Guangxi Provinces. The highest amount polyphenol was extracted by 70% ethanol at 70 °C 40 min with ultrasonic treatment. compound (VTP) determined...
The YidC protein of Escherichia coli is required for inserting Sec-independent membrane proteins and has a supportive role the insertion Sec-dependent into bilayer. Because portion copurifies with Sec translocase, this interaction might be necessary to assist in proteins. This study describes deletion analysis that investigates which parts are its SecDF complex translocase function as an insertase results suggest first periplasmic region, includes residues 24−346, particular SecF protein....
Significance NOD-like receptors (NLRs) are traditionally recognized as key surveillance pattern recognition (PRRs) during innate immune regulation. Several NLRs exhibit highly restricted expression in mammalian germline, where their physiological functions largely unknown. Here we report that Nlrp14, an NLR specifically expressed testis and ovary, plays a critical role regulating germ cell differentiation reproduction. Nlrp14 deficiency led to decreased primordial cell-like (PGCLC) vitro...
Mandatory newborn screening for metabolic disorders has not been implemented in most parts of China. Newborn mortality and morbidity could be markedly reduced by early diagnosis treatment inborn errors metabolism (IEM). Methods IEM tandem mass spectrometry (MS/MS) have developed, their advantages include rapid testing, high sensitivity, specificity, throughput, low sample volume (a single dried blood spot).Dried spots 100,077 newborns obtained from Jining city 2014-2015 were screened MS/MS....
Although interferon-gamma (IFN-γ) is known as a critical factor in polarizing macrophages into the pro-inflammatory state for immune response, how dietary flavonoids regulate IFN-γ response anti-inflammation incompletely elucidated. This study aims to investigate effect of fisetin, typical flavonol, on inhibition IFN-γ-induced inflammation by RNA sequencing (RNA-Seq) and cellular metabolism analysis. RAW264 pretreated with fisetin following stimulation were subjected RNA-Seq analyze...
Topogenic sequences direct the membrane topology of proteins by being recognized and decoded integral translocases. In this paper, we have compared minimal sequence characteristics helical-hairpin, reverse signal-anchor, stop-transfer in bacterial that use either YidC or SecYEG translocases for insertion. We find a stretch composed 3 leucines 16 alanines is required efficient membrane-anchoring M13 procoat protein inserts helical hairpin mechanism, only 19 has detectable ability. Similar...
Metabolomic analysis coupled with advanced genetic populations represents a powerful tool which to investigate the plant metabolome. However, analyses of rice (Oryza sativa) metabolome have been conducted mainly using natural accessions or single biparental population. Here, flag leaves from three interconnected chromosome segment substitution line common recurrent background were used dissect metabolic diversity. We effectively multiple populations, constructed by introducing genomic...
Abstract The mitogen‐activated protein kinase 4 (MKK4), a member of the MAP family, directly phosphorylates and activates c‐Jun NH2‐terminal kinases (JNK), in response to proinflammatory cytokines cellular stresses. Regulation MKK4 activity is considered be novel approach for prevention treatment inflammation. aim this study was identify whether fisetin, potential anti‐inflammatory compound, targets MKK4‐JNK cascade inhibit lipopolysaccharide (LPS)‐stimulated inflammatory response. RAW264...
Magnolol rich in Magnolia officinalis is a bioactive polyphenolic compound. The aim of this study was to examine the effects magnolol additive (MA) on growth performance, expression levels antioxidant-related genes, and intestinal mucosal morphology Linwu ducks aged from 49 70 days, comparing with that an antibiotic (colistin sulfate [CS]). A total 275, 49-day-old were assigned 5 groups cages 11 each fed diets supplemented 0, 100, 200 300 mg MA/kg CS/kg for 3 weeks, respectively. results...
AAV-mediated gene therapy typically requires a high dose of viral transduction, risking acute immune responses and patient safety, part which is due to limited understanding the host-viral interactions, especially post-transduction genome processing. Here, through genome-wide CRISPR screen, we identified SMCHD1 (Structural Maintenance Chromosomes Hinge Domain 1), an epigenetic modifier, as critical broad-spectrum restricting host factor for post-entry AAV transgene expression. knock-down by...
The M13 phage Procoat protein is one of the best characterized substrates for novel YidC pathway. It inserts into membrane independent SecYEG complex but requires 60 kDa protein. Mutant proteins with alterations in periplasmic region had been found to require and YidC. In this report, we show that insertion these mutants also strongly depends on SecDF bridges a cold-sensitive mutant YidC, Sec-dependent function impaired. We find specifically SecDF-dependent are inhibited strain. Finally,...
The allicin diallyldisulfid-S-oxide, a major garlic organosulfur compound (OSC) in crushed (Allium sativum L.), possesses antibacterial effects, and influences gut bacteria. In this study, we made allicin-free (AFG) extract investigated its effects on microbiome. C57BL/6N male mice were randomly divided into 6 groups fed normal diet (ND) high-fat (HFD) supplemented with or without AFG concentrations of 1% 5% for 11 weeks. genomic DNAs feces used to identify the microbiome by sequencing 16S...
This study aimed to investigate the subclinical symptom and histological lesions of 21-day-old 42-day-old broilers exposure low concentration aflatoxin B1 (AFB1), preventive effect with adsorbent (Toxo-MX) supplementation. A total 576 one-day-old Arbor Acres were randomly allotted into 6 treatments 8 replicates 12 birds per cage, fed 0 ppb, 60 ppb 120 AFB1 contamination diet or without Toxo-MX Results showed both significantly reduced growth performance in (P < 0.05), but not > however,...
Conditional lethal YidC mutants have been isolated to decipher the role of in assembly Sec-dependent and Sec-independent membrane proteins. We now show that insertion M13 procoat-lep protein is inhibited a short time temperature-sensitive mutant when shifted nonpermissive temperature. This provides an additional line evidence plays direct procoat protein. However, mutant, Pf3 phage coat leader peptidase were not strongly at restricted temperatures. Conversely, using cold-sensitive strain, we...
Abstract This study was conducted to explore the effect of dietary supplementation water-soluble extract rosemary (WER) on growth performance and intestinal health broilers infected with Eimeria tenella (E. tenella), evaluate anticoccidial activity WER. 360 1-d-old Chinese indigenous male yellow-feathered broiler chickens were randomly allocated six groups: blank control (BC) group (IC) received a basal diet; positive (PC) group, diet supplemented 200 mg/kg diclazuril; WER100, WER200, WER300...