A5062164180- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Ginseng Biological Effects and Applications
- Bioactive Compounds and Antitumor Agents
- Phytochemistry and biological activity of medicinal plants
- Toxin Mechanisms and Immunotoxins
- Phytochemicals and Antioxidant Activities
- Morinda citrifolia extract uses
- Bioactive natural compounds
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Genomics, phytochemicals, and oxidative stress
- Biochemical Analysis and Sensing Techniques
- Saffron Plant Research Studies
- Flavonoids in Medical Research
- Phytochemistry and Biological Activities
- melanin and skin pigmentation
- Chromatography in Natural Products
- Traditional Chinese Medicine Analysis
- Plant biochemistry and biosynthesis
- Biological Activity of Diterpenoids and Biflavonoids
- Inflammatory mediators and NSAID effects
- Natural Compound Pharmacology Studies
- Trypanosoma species research and implications
- Autophagy in Disease and Therapy
Nagasaki International University
2016-2025
Nagasaki University
2016
Japan Agency for Medical Research and Development
2016
University of Miyazaki
2016
Pharmac
2014
Kagoshima University
2004-2011
Medical University of South Carolina
2008-2010
Graduate School USA
2009
Japan Science and Technology Agency
2007
Anthocyanidins are the aglycon nucleuses of anthocyanins, which reddish pigments widely spread in colored fruits and vegetables. To investigate their anti-cancer effect, induction apoptosis was tested human promyelocytic leukemia cells (HL-60), is a valid model for testing antileukemic or general antitumoral compounds. Of six anthocyanidins representing aglycons most only those with an ortho-dihydroxyphenyl structure on B-ring induce apoptosis, suggesting that may contribute to apoptosis....
Eriobotrya japonica leaf is a traditional herbal medicine that contains numerous triterpenes, which have various pharmacological properties. In this study, we investigated the anti-proliferative activity of four triterpenes derived from E. japonica, including corosolic acid (CA), ursolic (UA), maslinic (MA) and oleanolic (OA), in human leukemia cell lines. CA showed strongest all lines tested, but not normal skin fibroblast To determine mechanism underlying effect CA, examined on molecular...
Trypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands new cases recorded annually. Control these infection(s) existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence resistant strains. Medicinal plants on other hand used in treatment various infectious diseases although their chemical properties not fully evaluated. In this study, we screened 112 crude extracts from 72...
Berberine (BBR), an isoquinoline alkaloid, is a well-known bioactive compound contained in medicinal plants used traditional and folk medicines. In this study, we investigated the subcellular localization apoptotic mechanisms of BBR were elucidated. First, confirmed incorporation into cell visually. showed antiproliferative activity promptly localized to nucleus from 5[Formula: see text]min 15[Formula: after treatment HL-60 human promyelocytic leukemia cells. Next, examined (1) its...
Eriobotryae folium (EF), the dried leaves of Eriobotrya japonica (Thunb.) Lindl. has been traditionally used to treat various diseases such as chronic bronchitis, cough, inflammation, skin diseases, and diabetes. In this study, we examined effects extract (EFE) on lipopolysaccharide (LPS)-induced production nitric oxide (NO) prostaglandin E2(PGE2) in RAW264 murine macrophage cells. EFE suppressed LPS-induced NO PGE2 a dose-dependent manner. Consistent with these observations, reduced...
Ginseng, an ancient and famous medicinal herb in the Orient, has been used as a valuable tonic for treatment of various diseases including hepatic disorders. Ginseng saponins, commonly known ginsenosides, are principal constituents have believed to be responsible multiple ginseng health benefits. There more 40 ginsenosides isolated from ginseng. To date, options common liver such cirrhosis, fatty liver, chronic hepatitis remain problematic. In this regard, extracts individual shown wide...
Recently, the resources of medicinal plants have been exhausting. The root Angelica acutiloba is one most important ingredients in Japanese Kampo medicine for treatment gynecological diseases. In our search alternative plant A. acutiloba, we found that its aerial part has anti-inflammatory potency as well root. Phytochemical investigation resulted isolation four compounds including a new dimeric phthalide, namely tokiaerialide (2), along with Z-ligustilide (1), falcarindiol (3), and...
Disruption of intestinal homeostasis can lead to inflammatory bowel diseases endowed susceptibility genes and environmental factors affecting accumulation activation colitogenic phagocytes. Plasmacytoid dendritic cells (pDCs) are immune that had been proposed control innate adaptive immunity through the massive secretion type I interferon (IFN-I). However, contribution pDCs progression inflammation remains unclear. Here we show a critical role in initiation acute colonic using...
Trypanosoma brucei parasites are kinetoplastid protozoa that devastate the health and economic well-being of millions people in Africa through disease human African trypanosomiasis (HAT). New chemotherapy has been eagerly awaited due to severe side effects drug resistance issues plaguing current drugs. Recently, there an emphasis on use medicinal plants worldwide. Morinda lucida Benth. is a popular plant widely distributed Africa, several research groups have reported antiprotozoal...
6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a major bioactive compound in wasabi ( Wasabia japonica ), which typical Japanese pungent spice. Recently, vivo and vitro studies demonstrated that 6-MSITC has several biological properties, including anti-inflammatory, antimicrobial, antiplatelet, anticancer effects. We previously reported strongly suppresses cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), cytokines, are important factors mediate inflammatory processes....
Liquiritin was reacted with a keyhole limpet hemocyanin (KLH) to synthesize liquiritin–KLH conjugate as an immunogen for mice. A hybridoma cell line named 2F8 secreted monoclonal antibody (mAb) against liquiritin, which applied enzyme-linked immunosorbent assay (ELISA) liquiritin. ELISA showed good linear range from 0.39 25 μg/mL of The maximum relative standard deviation (RSD) values the intra-assay and interassay were approximately 5%. recovery rates liquiritin in 100.9–103.7%,...
Childhood adrenoleukodystrophy (cALD) is a metabolic disorder in which very long-chain fatty acids (VLCFA) accumulate due to ALD protein gene defects, ultimately leading lipotoxicity-induced neuroinflammatory demyelinating disease. Therefore, we examined VLCFA-mediated alterations the metabolism of lipoxidative enzymes and inflammatory mediators cALD brain. 5-Lipoxygenase (5-LOX)-derived leukotrienes were significantly elevated all areas white matter Unlike cyclooxygenase-2 expression, was...
ABSTRACT Introduction Aristolochic acids (AAs) are naturally occurring nephrotoxicants and human carcinogens. acid I (AA‐I) aristolochic II (AA‐II) two important AAs with clear toxicity. Objective To obtain a monoclonal antibody (MAb) recognising AA‐I AA‐II develop an Eastern blotting technique for the specific visualisation easy determination of in plant extracts or tissues Aristolochia Asarum species. Methods A hybridoma secreting MAb against was prepared by cell fusion splenocytes derived...