A5100698274- Metallurgy and Material Forming
- Metal Forming Simulation Techniques
- RNA modifications and cancer
- RNA Research and Splicing
- High-Velocity Impact and Material Behavior
- Aluminum Alloy Microstructure Properties
- Microstructure and Mechanical Properties of Steels
- Mechanical Behavior of Composites
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Structural Response to Dynamic Loads
- Cellular and Composite Structures
- Aluminum Alloys Composites Properties
- Genomics and Phylogenetic Studies
- Additive Manufacturing Materials and Processes
- Fungal and yeast genetics research
- Transportation Safety and Impact Analysis
- HVDC Systems and Fault Protection
- Mechanical stress and fatigue analysis
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Metal Alloys Wear and Properties
- Additive Manufacturing and 3D Printing Technologies
- Radar Systems and Signal Processing
- RNA and protein synthesis mechanisms
Xi'an Jiaotong University
2020-2025
University of California, Riverside
2019-2024
Tianjin Medical University General Hospital
2023-2024
Chinese Academy of Sciences
2016-2024
Beihang University
2024
Beijing Institute of Radio Metrology and Measurement
2023-2024
University of Birmingham
2024
Dalian University of Technology
2024
Tianjin Medical University
2024
Second Hospital of Tianjin Medical University
2024
Understanding the relationship between millions of functional DNA elements and their protein regulators, how they work in conjunction to manifest diverse phenotypes, is key advancing our understanding mammalian genome. Next-generation sequencing technology now used widely probe these protein-DNA interactions profile gene expression at a genome-wide scale. As cost continues fall, interpretation ever increasing amount data generated represents considerable challenge. We have developed ngs.plot...
ChIP-seq is increasingly being used for genome-wide profiling of histone modification marks. It particular importance to compare data two different conditions, such as disease vs. control, and identify regions that show differences in ChIP enrichment. We have developed a powerful easy use program, called diffReps, detect those differential sites from data, with or without biological replicates. In addition, we useful tools analysis the diffReps package: one annotation other finding chromatin...
Abstract Background Bamboo is one of the most important nontimber forestry products worldwide. However, a chromosome-level reference genome lacking, and an evolutionary view alternative splicing (AS) in bamboo remains unclear despite emerging omics data improved technologies. Results Here, we provide de novo assembly moso (Phyllostachys edulis) using additional abundance sequencing Hi-C scaffolding strategy. The significantly scaffold N50 79.90 Mb, approximately 243 times longer than...
Abstract Background Increasing evidence supports a role for altered gene expression in mediating the lasting effects of cocaine on brain, and recent work has demonstrated involvement chromatin modifications these alterations. However, all such studies to date have been restricted by their reliance microarray technologies that intrinsic limitations. Results We use next generation sequencing methods, RNA-seq ChIP-seq RNA polymerase II several histone methylation marks, obtain more complete...
Abstract Microsatellite repeat expansions within genes contribute to a number of neurological diseases 1,2 . The accumulation toxic proteins and RNA molecules with repetitive sequences, and/or sequestration RNA-binding by containing expanded repeats are thought be important contributors disease aetiology 3–9 Here we reveal that the adenosine in CAG can methylated N 1 -methyladenosine (m A) TRMT61A, m A demethylated ALKBH3. We also observed A/adenosine ratio increases length, which is...
N6-methyladenosine (m6A) is the most abundant internal modification in mRNA and this methylation constitutes an important regulatory mechanism for stability translational efficiency of mRNA. In study, we found that protein levels adenylate kinase 4 (AK4) m6A writer METTL3 are significantly higher tamoxifen-resistant (TamR) MCF-7 cells than parental cells. The TamR also exhibit increased at multiple consensus motif sites 5' untranslated region (5' UTR) AK4 mRNA, genetic depletion led to a...
Dissimilar aluminium alloy-tailor weld blanks (DA-TWB) are increasingly being used in transportation industries due to their significant benefit reinforcing vehicle structures as well improving lightweight performance. Hot stamping technologies enabling the manufacture of pressings from DA-TWB have yet be developed and widely implemented. In present research, a unified automated production system was integrate functions heating, blank transfer, forming post-form heat treatment, automatically...
RUNX1–RUNX1T1 (formerly AML1-ETO), a transcription factor generated by the t(8;21) translocation in acute myeloid leukemia (AML), dictates leukemic program increasing self-renewal and inhibiting differentiation. Here we demonstrate that histone demethylase JMJD1C functions as coactivator for is required its transcriptional program. directly recruited to target genes regulates their expression maintaining low H3K9 dimethyl (H3K9me2) levels. Analyses knockout mice also establish requirement...
Sequencing of the 3′ end poly(A) + RNA identifies cleavage and polyadenylation sites (pAs) measures transcript expression. We previously developed a method, region extraction deep sequencing (3′READS), to address mispriming issues that often plague sequencing. Here we report new version, named 3′READS+, which has vastly improved accuracy sensitivity. Using special locked nucleic acid oligo capture remove bulk tail, 3′READS+ generates fragments with an optimal number terminal A's balance data...
Abstract Sustainability is a key factor in an automotive OEMs’ business strategy. Vehicle electrification particular has received increased attention, and major manufacturers have already undertaken significant investments this area. However, order to fully confront the sustainability challenge industry, lightweight design additional alternative propulsion technologies also required. weight closely correlated with fuel consumption range for internal combustion electrified vehicles,...
Alternative polyadenylation (APA) is a mechanism that generates multiple mRNA isoforms with different 3'UTRs and/or coding sequences from single gene. Here, using 3' region extraction and deep sequencing (3'READS), we have systematically mapped cleavage sites (PASs) in Drosophila melanogaster, expanding the total repertoire of PASs previously identified for species, especially those located A-rich genomic sequences. Cis-element analysis revealed distinct sequence motifs around fly when...
Termination of RNA polymerase II (RNAPII) transcription is associated with 3' end formation. For coding genes, termination initiated by the cleavage/polyadenylation machinery. In contrast, a majority noncoding events in Saccharomyces cerevisiae does not rely on cleavage for but instead terminates via pathway that requires Nrd1-Nab3-Sen1 (NNS) complex. Here we show Schizosaccharomyces pombe ortholog Nrd1, Seb1, function NNS-like promotes polyadenylation site selection and genes. We found Seb1...
Crashworthiness of a civil airplane fuselage section was studied in this paper. Firstly, the failure criterion rivet by test, showing that ultimate tension and shear loads were obviously affected loading speed. The relations between speed average shear, expressed two logarithmic functions. Then, vertical drop test conducted with an actual impact velocity 6.85 m/s, meanwhile deformation cabin frame accelerations at typical locations measured. finite element model main structure developed...
Alternative polyadenylation (APA) is a widespread mechanism that generates mRNA isoforms with distinct properties. Here we have systematically mapped and compared cleavage sites (PASs) in two yeast species, S. cerevisiae pombe . Although >80% of the genes each species were found to display APA, showed greater 3′ UTR size differences among APA than did PASs different locations gene are surrounded sequences both often associated motifs involved Nrd1-Nab3-Sen1 termination pathway. In pombe,...
Abstract Termination of RNA polymerase II (RNAPII) transcription is a fundamental step gene expression that critical for determining the borders between genes. In budding yeast, termination at protein-coding genes initiated by cleavage/polyadenylation machinery, whereas most noncoding (ncRNA) occurs via Nrd1–Nab3–Sen1 (NNS) pathway. Here, we find NNS-like not conserved in fission yeast. Rather, genome-wide analyses show global recruitment mRNA 3′ end processing factors ncRNA genes, including...
Long-lasting forms of synaptic plasticity that underlie learning and memory require new transcription translation for their persistence. The remarkable polarity compartmentalization neurons raises questions about the spatial temporal regulation gene expression within neurons. Alternative cleavage polyadenylation (APA) generates mRNA isoforms with different 3' untranslated regions (3'UTRs) and/or coding sequences. Changes in 3'UTR composition mRNAs can alter by regulating transcript...
The measurement and control of the heat transfer sheet alloys in successive non-isothermal forming cycles is crucial to achieve desired post-form properties microstructure, which could not as yet be realized by using traditional test facilities. In present research, a novel facility was designed generate subsequently measure in-situ from alloy multi-mediums such tools, air, lubricant coating. More importantly, able use single sample perform cycles, obtain high throughput experimental results...