A5010518637- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Neurotransmitter Receptor Influence on Behavior
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Nuclear Receptors and Signaling
- Stress Responses and Cortisol
- Neuroendocrine regulation and behavior
- RNA regulation and disease
- Pluripotent Stem Cells Research
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Bioinformatics and Genomic Networks
- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Autism Spectrum Disorder Research
- Sirtuins and Resveratrol in Medicine
- Cannabis and Cannabinoid Research
University of Pennsylvania
2016-2025
Translational Therapeutics (United States)
2017-2025
Children's Hospital of Philadelphia
2023
College of New Rochelle
2023
Hôpital Saint-Louis
2023
Ibis Reproductive Health
2022
California University of Pennsylvania
2021
Icahn School of Medicine at Mount Sinai
2013-2018
Allen Institute for Brain Science
2013-2018
Boston College
2013-2014
Many behavioral and electrophysiological studies in animals humans have suggested that sleep circadian rhythms influence memory consolidation. In rodents, hippocampus-dependent may be particularly sensitive to deprivation after training, as spatial the Morris water maze is impaired by rapid eye movement following training. Spatial learning maze, however, requires multiple training trials performance, measured time reach hidden platform influenced not only but also procedural learning. To...
Abstract Antidepressants (ADs) are the most common treatment for major depressive disorder (MDD). However, only ∼30% of patients experience adequate response after a single AD trial, and this variability remains poorly understood. Here, we investigated microRNAs (miRNAs) as biomarkers using small RNA-sequencing in paired samples from MDD enrolled large, randomized placebo-controlled trial duloxetine collected before 8 weeks treatment. Our results revealed differential expression miR-146a-5p,...
Sirtuins (SIRTs), class III histone deacetylases, are well characterized for their control of cellular physiology in peripheral tissues, but influence brain under normal and pathological conditions remains poorly understood. Here, we establish an essential role SIRT1 SIRT2 regulating behavioral responses to cocaine morphine through actions the nucleus accumbens (NAc), a key reward region. We show that chronic administration increases expression mouse NAc, while induces alone, with no...
Previous studies have shown that chronic cocaine administration induces SIRT1, a Class III histone deacetylase, in the nucleus accumbens (NAc), key brain reward region, and such induction influences gene regulation place conditioning effects of cocaine. To determine mechanisms by which SIRT1 mediates cocaine-induced plasticity NAc, we used chromatin immunoprecipitation followed massively parallel sequencing (ChIP-seq), 1 d after 7 daily (20 mg/kg) or saline injections, to map binding...
Recent studies have implicated epigenetic remodeling in brain reward regions following psychostimulant or stress exposure. It has only recently become possible to target a given type of single gene interest, and probe the functional relevance such regulation neuropsychiatric disease. We sought examine role histone modifications at murine Cdk5 (cyclin-dependent kinase 5) locus, growing evidence expression nucleus accumbens (NAc) influencing reward-related behaviors. Viral-mediated delivery...
Abstract Endogenous homeostatic mechanisms can restore normal neuronal function following cocaine-induced neuroadaptations. Such may be exploited to develop novel therapies for cocaine addiction, but a molecular target has not yet been identified. Here we profiled mouse gene expression during early and late abstinence identify putative regulators of neural homeostasis. Cocaine activated the transcription factor, Nr4a1 , its gene, Cartpt key molecule involved in dopamine metabolism. Sustained...
Abstract Alternative splicing of neuronal genes is controlled partly by the coordinated action polypyrimidine tract binding proteins (PTBPs). While PTBP1 ubiquitously expressed, PTBP2 predominantly neuronal. Here, we define footprint in human transcriptome using brain tissue and induced pluripotent stem cell-derived neurons (iPSC-neurons). We map sites, characterize PTBP2-dependent alternative events, identify novel targets including SYNGAP1 , a synaptic gene whose loss-of-function leads to...
Abstract The serotonin 2 receptor (5HT2R) agonist psilocybin displays rapid and persistent therapeutic efficacy across neuropsychiatric disorders characterized by cognitive inflexibility. However, the impact of on patterns neural activity underlying sustained changes in behavioral flexibility has not been characterized. To test hypothesis that enhances altering cortical ensembles, we performed longitudinal single-cell calcium imaging retrosplenial cortex a five-day trace fear learning...
SummaryThe effects of ethanol on hepatic oxygenation were measured by vein catheterization in the baboon. Chronic alcohol administration resulted slightly lower oxygen tension vein, but not ischemia, when after an overnight fast was no longer present blood. Intravenous increase regardless prior exposure to or presence liver disease. The increased associated with a marked blood flow. These data conjunction observation progressive injury alcohol-fed animals support concept that alcoholic can...
A growing number of studies implicate the brain9s reward circuitry in aggressive behavior. However, cellular and molecular mechanisms within brain regions that modulate intensity aggression as well motivation for it have been underexplored. Here, we investigate cell-type-specific influence ΔFosB, a transcription factor known to regulate range motivated behaviors, acting nucleus accumbens (NAc), key region, male mice. We show ΔFosB is specifically increased dopamine D1 receptor...
The severity of Alzheimer's disease (AD) progression involves a complex interplay genetics, age, and environmental factors orchestrated by histone acetyltransferase (HAT)-mediated neuroepigenetic mechanisms. While disruption Tip60 HAT action in neural gene control is implicated AD, alternative mechanisms underlying function remain unexplored. Here, we report novel RNA binding for addition to its function. We show that preferentially interacts with pre-mRNAs emanating from chromatin targets...
Opioid use disorder constitutes a major health and economic burden, but our limited understanding of the underlying neurobiology impedes better interventions. Alteration in activity output dopamine (DA) neurons ventral tegmental area (VTA) contributes to drug effects, mechanisms these changes remain relatively unexplored. We used translating ribosome affinity purification RNA sequencing identify gene expression mouse VTA DA following chronic morphine exposure. found that neuropeptide...