A5082491541- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Pain Management and Placebo Effect
- Pharmacological Receptor Mechanisms and Effects
- Pediatric Pain Management Techniques
- Neurotransmitter Receptor Influence on Behavior
- Cancer, Stress, Anesthesia, and Immune Response
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Psychedelics and Drug Studies
- Stress Responses and Cortisol
- Ion channel regulation and function
- Veterinary Pharmacology and Anesthesia
- Pain Management and Opioid Use
- Sleep and Wakefulness Research
- Pancreatic function and diabetes
- Chemical synthesis and alkaloids
- Computational Drug Discovery Methods
- Musculoskeletal pain and rehabilitation
- Anesthesia and Neurotoxicity Research
- Exercise and Physiological Responses
- Pharmacogenetics and Drug Metabolism
- bioluminescence and chemiluminescence research
- Liver Disease Diagnosis and Treatment
University of Pennsylvania
2020-2025
California University of Pennsylvania
2023-2024
Stanford University
2016-2019
Neurosciences Institute
2016-2019
University of Kentucky
2010-2019
Palo Alto University
2018
The emotional dimension of pain unpleasantness is an phenomenon distinct from pain's sensory qualities. To study how the brain processes pain-related emotions, Corder et al. used in vivo neural calcium imaging freely behaving mice. They identified circuits that respond to and directly tested their causal role motivational behaviors associated with acute chronic pain. Science , this issue p. 276
Pain and Dependence The properties functions of µ-opioid receptors have been studied intensively with respect to the binding endogenous or exogenous ligands. However, much less is known about constitutive, ligand-independent, activation opioid receptors. Working in mice, Corder et al. (p. 1394 ) observed prolonged constitutive spinal dorsal horn after transient peripheral inflammation. results suggest that depresses nociception—the perception pain—for long periods time induces cellular...
Cellular interactions between delta and mu opioid receptors (DORs MORs), including heteromerization, are thought to regulate analgesia. However, the identity of nociceptive neurons in which such could occur vivo remains elusive. Here we show that DOR-MOR co-expression is limited small populations excitatory interneurons projection spinal cord dorsal horn unexpectedly predominates ventral motor circuits. Similarly, rare parabrachial, amygdalar, cortical brain regions processing information....
Dramatically up-regulated in the dorsal horn of mammalian spinal cord following inflammation or nerve injury, neuropeptide Y (NPY) is poised to regulate transmission sensory signals. We found that doxycycline-induced conditional vivo ( Npy tet/tet ) knockdown NPY produced rapid, reversible, and repeatable increases intensity duration tactile thermal hypersensitivity. Remarkably, when allowed resolve for several weeks, behavioral hypersensitivity could be dramatically reinstated with...
Objective and automatic measurement of pain in mice remains a barrier for discovery neuroscience. Here, we capture paw kinematics during behavior with high-speed videography automated tracking machine deep learning approaches. Our statistical software platform, PAWS (Pain Assessment at Withdrawal Speeds), uses univariate projection position over time to automatically quantify seven behavioral features that are combined into single, score. Automated reveals behaviorally divergent mouse strain...
Abstract The serotonin 2 receptor (5HT2R) agonist psilocybin displays rapid and persistent therapeutic efficacy across neuropsychiatric disorders characterized by cognitive inflexibility. However, the impact of on patterns neural activity underlying sustained changes in behavioral flexibility has not been characterized. To test hypothesis that enhances altering cortical ensembles, we performed longitudinal single-cell calcium imaging retrosplenial cortex a five-day trace fear learning...
Abstract Endogenous neuropeptide Y (NPY) exerts long-lasting spinal inhibitory control of neuropathic pain, but its mechanism action is complicated by the expression receptors at multiple sites in dorsal horn: NPY Y1 (Y1Rs) on post-synaptic neurons and both Y1Rs Y2Rs central terminals primary afferents. We found that Y1R-expressing contain markers excitatory not interneurons rat superficial horn. To test relevance this population to development and/or maintenance acute we selectively ablated...
Background Nociceptive and neuropathic pain occurs as part of the disease process after traumatic brain injury (TBI) in humans. Central peripheral inflammation, a major secondary initiated by event, has been implicated potentiation nociceptive pain. We hypothesized that inflammatory response to diffuse potentiates persistent through prolonged immune dysregulation. Results To test this, adult, male C57BL/6 mice were subjected midline fluid percussion or sham procedure. One cohort was analyzed...
The basolateral amygdala (BLA) is essential for assigning positive or negative valence to sensory stimuli. Noxious stimuli that cause pain are encoded by an ensemble of
Opioids initiate dynamic maladaptation in brain reward and affect circuits that occur throughout chronic exposure withdrawal persist beyond cessation. Protracted abstinence is characterized by negative affective behaviors such as heightened anxiety, irritability, dysphoria, anhedonia, which pose a significant risk factor for relapse. While the ventral tegmental area (VTA) mu-opioid receptors (MORs) are critical opioid reinforcement, specific contributions of VTA MOR neurons mediating...
With concurrent global epidemics of chronic pain and opioid use disorders, there is a critical need to identify, target manipulate specific cell populations expressing the mu-opioid receptor (MOR). However, available tools transgenic models for gaining long-term genetic access MOR+ neural types circuits involved in modulating pain, analgesia addiction across species are limited. To address this, we developed catalog MOR promoter (MORp) based constructs packaged into adeno-associated viral...
Abstract Tissue injury induces a long‐lasting latent sensitization (LS) of spinal nociceptive signaling that is kept in remission by an opposing µ‐opioid receptor (MOR) constitutive activity. To test the hypothesis supraspinal sites become engaged, we induced hindpaw inflammation, waited 3 weeks for mechanical hypersensitivity to resolve, and then injected opioid inhibitors naltrexone, CTOP or β‐funaltrexamine subcutaneously, and/or into cerebral ventricles. Intracerebroventricular injection...
The anterior cingulate cortex plays a pivotal role in the cognitive and affective aspects of pain perception. Both endogenous exogenous opioid signaling within mitigate cortical nociception, reducing unpleasantness. However, specific functional molecular identities cells mediating analgesia remain elusive. Given complexity as sensory emotional experience, richness ethological pain-related behaviors, we developed standardized, deep-learning platform for deconstructing behavior dynamics...
Key targets of both the therapeutic and abused properties opioids are μ-opioid receptors (MORs). Despite years research investigating biochemistry signal transduction pathways associated with MOR activation, we do not fully understand cellular mechanisms underlying opioid addiction. Given that addictive such as morphine, oxycodone, heroin, fentanyl all activate MORs, current therapies naloxone buprenorphine block this availability tools to mechanistically investigate opioid-mediated...