A5051298333- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Ultrasound and Hyperthermia Applications
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- Wnt/β-catenin signaling in development and cancer
- Ecology and Conservation Studies
- Psoriasis: Treatment and Pathogenesis
- RNA Research and Splicing
- Reservoir Engineering and Simulation Methods
- dental development and anomalies
- Ultrasound Imaging and Elastography
- Urticaria and Related Conditions
- Advanced Biosensing Techniques and Applications
- Korean Urban and Social Studies
- Environmental Policies and Emissions
- Flood Risk Assessment and Management
- Photoreceptor and optogenetics research
- Environmental and Social Impact Assessments
- Urban Heat Island Mitigation
- Cutaneous Melanoma Detection and Management
- Marine and Coastal Research
Rice University
2020-2024
University of Southern California
2017
Kangwon National University
2012
Chungnam National University
2010-2011
Engineered base editors convert the second nucleotide C to T in context of 5′-CC-3′ with high precision and targeting fidelity.
Hypoxia plays a central role in cancer progression and resistance to therapy. We have engineered microdevice platform recapitulate the intratumor oxygen gradients that drive heterogeneous hypoxic landscapes solid tumors. Our design features "tumor section"-like culture by incorporating cell layer between two diffusion barriers, where an gradient is established cellular metabolism physical constraints. confirmed numerical simulation imaging-based sensor measurement. also demonstrated...
Gene expression is a critical component of brain physiology, but monitoring this in the living represents major challenge. Here, we introduce new paradigm called recovery markers through insonation (REMIS) for noninvasive measurement gene with cell type, spatial, and temporal specificity. Our approach relies on engineered protein that are produced neurons exit into brain’s interstitium. When ultrasound applied to targeted regions, it opens blood-brain barrier releases these bloodstream. Once...
Through differentiation processes, keratinocytes provide a physical barrier to our bodies and control skin features such as moisturization, wrinkles pigmentation. Keratinocyte is disturbed in several diseases psoriasis atopic dermatitis.The aim of this study evaluate the keratinocyte differentiation-enhancing effect rose absolute oil (RAO).Primary cultured human normal were treated with RAO, then checked by expression marker genes.RAO did not induce cytotoxicity on at dose 10microM. The...
ABSTRACT Focused Ultrasound Blood-Brain Barrier Opening (FUS-BBBO) can deliver adeno-associated viral vectors (AAVs) to treat genetic disorders of the brain. However, such often affect large brain regions. Moreover, applicability FUS-BBBO in treatment brain-wide has not yet been evaluated. Herein, we evaluated transduction efficiency and safety opening up 105 sites simultaneously. Increasing number targeted increased gene delivery at each site. We achieved 60% cells with comparable majority...
Blood tests are among the most common clinical tools due to their low cost, simplicity, and ability observe many markers at once. However, currently, blood can only monitor a fraction of physiological processes that happen have serum marker. Here, we will present our work on development synthetic track gene expression in intact brain cells with simple test. The marker approach has several advantages. First, detection existing may be challenging levels blood. With marker, one design reporter...
ABSTRACT Understanding transcription profiles of living tissues is critical for biology and medicine. However, measurement the transcript levels typically done in homogenized post-mortem. Here, we present a new platform that enables non-invasive monitoring specific mRNA vivo , without tissue destruction. We achieved this by combining two cutting-edge tools - synthetic serum markers, called Released Markers Activity ( RMAs ), RNA-based sensors transcription. call IN-vivo Tracking ACtive...
선진국에서의 강변여과수 개발은 150년 정도의 역사를 가지고 있다. 한국에서도 지속적으로 증가하고 있는 용수수요에 안정적으로 대처하기 위한 원수 확보방안으로 강변여과수에 대한 조사가 1990년대부터 4대강 유역을 중심으로 시작되었으며, 현재 낙동강을 강변여과를 활용한 상수도 공급이 이루어지고 본 논문은 서울의 원수수질 안정을 방안으로 강변여과 도입의 타당성에 연구이다. 개발 적지의 선정을 위해 여러 가지 속성들을 계층적으로 분류하고 각 속성의 중요도를 파악함으로써 최적 대안을 선정하는 계층분석과정기법(AHP)을 적용하였다. 한강 유역의 경우 개발후보지역을 대상으로 적지분석을 실시한 결과, 수질 및 기존시설연계성 등에서 유리한 광나루지구가 최적지로 선정되었다. Riverbank filtration for water supply has been employed about 150 years in developed countries. In Korea, the feasibility...
최근 지표수의 취수원 다변화 및 수질에 대한 불신 등으로 강변여과 개발에 관심이 모아지고 있다. 선진국에서의 개발은 150년 정도의 역사를 가지고 있으며, 우리나라에서도 지속적으로 증가하고 있는 용수수요에 안정적으로 대처하기 위한 원수 확보방안으로 강변여과에 조사가 1990년대부터 시작되었고, 현재 낙동강을 중심으로 몇몇 지자체에서는 강변여과를 활용한 상수도 공급이 이루어지고 그러나 지금까지는 의한 개발가능량 산정과 관정개발에 연구가 집중되어 개발가능지 선정에 관한 체계적인 이루어지지 않고 실정이다. 본 논문에서는 게임이론을 개발 적지선정에 대해 연구하였다. 게임이론은 수학적 분석이론의 하나로 사회과학(특히 경제학)과 생물학, 공학, 컴퓨터과학 등에 적용되고 연구에서는 개발을 적정 후보지 결정에 이용되었다. 제안된 정책모형은 확률적 접근을 시도한 새로운 방법론이며, 적지선정을 효율적인 분석이 가능한 것으로 나타났다. The tap water supply in Korea...
ABSTRACT Noninvasive efforts to map brain gene expression have been hampered by low sensitivity and limited access the brain. Here, we introduce a new platform that enables multiplexed, noninvasive, site-specific monitoring of through novel class engineered reporters called Released Markers Activity (RMAs). Instead detecting in less accessible brain, RMA exit from known region into blood, where they can be easily measured with biochemical techniques. Expressing RMAs at single site, typically...
한국에서는 증가하는 용수수요에 대처하기 위해 1990년대부터 낙동강 유역의 지자체들에서 강변여과수를 활용하고 있다. 본 연구에서는 서울의 원수수질 안정을 위한 방안으로 강변여과 도입의 타당성을 검토하였다. 선행 논문에서 계층분석과정(AHP)에 의해 선택된 광나루지구에 대한 개발가능량 평가를 지하수 모델링이 수행되었다. 광나루지구에서는 생태계보존지역 등을 고려하여 하천부지 약 1,200m 구간에 대해 관정시스템을 구축할 수 있을 것으로 파악되었다. 취수량을 늘리기 인공호수의 조성이 제안되었다. 80m 간격으로 16개의 관정을 설치할 경우 적정개발량은 연간 2,336만<TEX>$m^3$</TEX>으로 산정되었다. In Korea, riverbank filtration has drawn attention since 1990's as an alternative having potential to stably meet the ever-increasing water demand....
Gene expression is a critical component of brain physiology and activity, but monitoring this in the living represents significant challenge. Here, we introduce new paradigm called Recovery Markers through InSonation (REMIS) for noninvasive measurement gene with cell-type, spatial, temporal specificity. Our approach relies on engineered protein markers that are designed to be expressed neurons exit into interstitium. By applying ultrasound targeted regions, these released bloodstream, where...
Although more than 90% of melanomas have cutaneous origins, sometimes present metastatically with no apparent primary lesion. A 62-year-old female presented black pigmentation on her left thumbnail that had begun 2 years earlier and after the biopsy, she was diagnosed malignant melanoma. Interestingly, 7 earlier, a 4 cm palpable mass axilla been as melanoma from an unknown site (MUP) involvement axillary lymph node. We speculate thumb in metastasis thumb, suggest several hypotheses...