A5006640426- Dental materials and restorations
- Dental Implant Techniques and Outcomes
- Enzyme Production and Characterization
- Dental Erosion and Treatment
- Bone Tissue Engineering Materials
- Orthodontics and Dentofacial Orthopedics
- Nanoplatforms for cancer theranostics
- Microbial Metabolites in Food Biotechnology
- Graphene and Nanomaterials Applications
- Biofuel production and bioconversion
- Vehicle emissions and performance
- Nanoparticles: synthesis and applications
- Laser-Plasma Interactions and Diagnostics
- Plant Stress Responses and Tolerance
- Antifungal resistance and susceptibility
- Microbial Natural Products and Biosynthesis
- CRISPR and Genetic Engineering
- MXene and MAX Phase Materials
- Alkaloids: synthesis and pharmacology
- Wastewater Treatment and Nitrogen Removal
- Peptidase Inhibition and Analysis
- Selenium in Biological Systems
- Protease and Inhibitor Mechanisms
- Extracellular vesicles in disease
- Air Quality Monitoring and Forecasting
Jiangnan University
2013-2024
Shanghai University
2024
Vanderbilt University
2024
Capital Medical University
2012-2023
State Key Laboratory of Food Science and Technology
2021
Rice University
2020
Henan Agricultural University
2013
Tongji University
2012
State Key Laboratory of Pollution Control and Resource Reuse
2012
Engineered base editors convert the second nucleotide C to T in context of 5′-CC-3′ with high precision and targeting fidelity.
This study aims to investigate the effect of sandblasting on surface roughness zirconia and shear bond strength veneering porcelain. Pre-sintered plates were prepared divided into four groups. Group A not treated at all; group B first sandblasted under 0.2 MPa pressure then densely sintered; C D sintered first, 0.4 pressures respectively. Surface was measured 3D reconstructed for specimens, which also analyzed with X-ray diffractometry. Finally after porcelain sintering, tests conducted....
This investigation explored the prospective efficacy of 2D Ti 3 C 2 MXene nanosheets in promoting osteogenic differentiation BMSCs through photothermal conversion.
A dedicated enzyme for the formation of central C ring in tetracyclic ergoline clinically important ergot alkaloids has never been found. Herein, we report a dual role catalase (EasC), unexpectedly using O2 as oxidant, that catalyzes oxidative cyclization from 1,3-diene intermediate. Our study showcases how nature evolves common enantioselective C–C bond construction complex polycyclic scaffolds.
Quinolactacins are novel fungal alkaloids that feature a quinolone-γ-lactam hybrid, which is potential pharmacophore for the treatment of cancer and Alzheimer's disease. Herein, we report identification quinolactacin A2 biosynthetic gene cluster elucidate enzymatic basis formation structure. We reveal an unusual β-keto acid (N-methyl-2-aminobenzoylacetate) precursor derived from primary metabolite l-kynurenine via methylation, oxidative decarboxylation, amide hydrolysis reactions. In vitro...
Marine extremophiles produce cold-adapted and/or salt-tolerant enzymes to survive in harsh conditions. These are naturally evolved with unique structural features that confer a high level of flexibility, solubility and substrate-binding ability compared mesophilic thermostable homologs. Here, we identified characterized an amylase, SdG5A, from the marine bacterium Saccharophagus degradans 2-40 T . We expressed protein Bacillus subtilis found purified SdG5A enabled highly specific production...
Maltooligosaccharide-forming amylases (MFAs) hydrolyze starch into maltooligosaccharides with a defined degree of polymerization. However, the enzymatic mechanism underlying product specificity remains partially understood. Here, we show that Saccharophagus degradans MFA (SdMFA) contains noncatalytic starch-binding domain (SBD), which belongs to carbohydrate-binding module family 20 and enables modulation specificity. Removal SBD from SdMFA resulted in 3.5-fold lower production target...
Both pro-inflammatory M1 and anti-inflammatory M2 macrophages play vital roles in the immune response during bone tissue regeneration. Current biomaterials are designed to promote macrophage polarization by manipulating various physical properties. However, this approach lacks precision controlling initiation of immunomodulation, potentially leading premature suppression. In study, photothermal effect Ti3C2Tx MXene nanosheets under near-infrared (NIR) irradiation was employed achieve remote...
Cold-adapted bidomain enzymes are vital for transforming modern industries by decreasing energy consumption, delivering economic benefits, and fostering sustainability through reduced greenhouse gas emissions. Yet, the design strategies guiding their acquisition of cold adaptation remain unknown. Here, we developed an integrated computational-experimental strategy to engineer enhanced cold-adaptation. Using five model amylase variants exhibiting different degrees adaptation, identified a...
Aminopeptidases can selectively catalyze the cleavage of N-terminal amino acid residues from peptides and proteins. Bacillus subtilis aminopeptidase (BSAP) is most active toward p-nitroanilides (pNAs) derivatives Leu, Arg, Lys. The BSAP with broad substrate specificity expected to improve its application. Based on an analysis predicted structure BSAP, four (Leu 370, Asn 385, Ile 387, Val 396) located in binding region were selected for saturation mutagenesis. hydrolytic activity different...
This study aimed to investigate the effects of subpressure on bond properties total-etching adhesive dentin. Thirty-six caries-free premolars were sectioned parallel occlusal plane and randomly divided into four groups (n = 9): a control group (C, no treatment) three groups, which treated under 0.8, 0.6 or 0.4 bar after applying adhesives, named S
This research evaluated the effects of subpressure on shear bond strength (SBS) 80 specimens with flat enamel surfaces and AgNO 3 microleakage 40 1 mm deep cavities before after thermocycling. The 168 was grounded to a surface. Two types sealants (E H) were selected. Sealants applied surface (88 specimens, group F) either subjected or not subpressure. bonding interfaces observed using scanning electron microscopy (SEM) SBS examined universal testing machine failure mode also analyzed. For...
The Z-pinch implosion from a wire array load is one of the ways to generate strong x-ray radiation. Under conditions pulsed power accelerator, radiation yield determined by initial diameter chosen and mass per length load. In this paper, effects mass, diameter, as well material on time, trajectory, velocity kinetic energy are estimated using an infinitely thin annular shell model. relationship among parameters analyzed. optimized obtained under given driven current. Our conclusions model in...