A5054969822- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Molecular Sensors and Ion Detection
- Amino Acid Enzymes and Metabolism
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Biofuel production and bioconversion
- Protein Hydrolysis and Bioactive Peptides
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Analytical Chemistry and Sensors
- Phytase and its Applications
- Hydrogels: synthesis, properties, applications
- Pancreatic function and diabetes
- Luminescence and Fluorescent Materials
- Gene Regulatory Network Analysis
- Toxin Mechanisms and Immunotoxins
- Sulfur Compounds in Biology
- Protein purification and stability
- Biochemical and Molecular Research
- Corneal Surgery and Treatments
Jiangnan University
2015-2024
Wuhan University of Science and Technology
2023-2024
Guizhou Center for Disease Control and Prevention
2024
Chengdu University of Traditional Chinese Medicine
2024
Research Institute of Tropical Forestry
2023
Chinese Academy of Forestry
2023
South China University of Technology
2023
Minzu University of China
2023
Changsha University of Science and Technology
2020-2021
Hainan Medical University
2021
By employing a dual response approach, distinguishable fluorescence signals are initiated by GSH-mediated and Cys/Hcy-induced cascade reactions, thus allowing selective detection.
Synbiotics are increasingly used by the general population to boost immunity. However, there is limited evidence concerning immunomodulatory effects of synbiotics in healthy individuals. Therefore, we conducted a double-blind, randomized, placebo-controlled study 106 adults. Participants were randomly assigned receive either (containing Bifidobacterium lactis HN019 1.5 × 108 CFU/d, Lactobacillus rhamnosus HN001 7.5 107 and fructooligosaccharide 500 mg/d) or placebo for 8 weeks. Immune...
Abstract Allergic rhinitis (AR) remains a major health problem worldwide. Compared with traditional oral drugs, nasal administration avoids first-pass metabolism and achieve faster more effective efficacy. In this study, we used the ion crosslinking method to prepare quercetin–chitosan adaptive nanomedicine (QCS) delivery system evaluated in treatment of allergic mice models. The obtained positively charged nanoparticles particle size 229.2 ± 0.2 nm have excellent characteristics...
Escherichia coli NZN111 is a pflB ldhA double mutant which loses its ability to ferment glucose anaerobically due redox imbalance. In this study, two-stage culture of was carried out for succinic acid production. It found that when aerobically cultured on acetate, it regained the with as major product in subsequent anaerobic culture. flasks, produced at level 11.26 g/liter from 13.4 yield 1.28 mol/mol and productivity 1.13 g/liter.h stage. Analyses key enzyme activities revealed isocitrate...
A series of new platinum(II) 5,15-bis(pentafluorophenyl)-10,20-bis(phenyl)porphyrin-9,9-dioctylfluorene copolymers, in which the relative intensities blue fluorescence and red phosphorescence can be easily tuned by initial feed ratio two monomers or energy transfer between fluorescent phosphorescent units, have been designed prepared for application ratiometric dual emissive oxygen sensing. To best our knowledge, this is first example a sensor based on fluorescent/phosphorescent polymers...
Bacillus subtilis is an all-important Gram-positive bacterium of valuable biotechnological utility that has been widely used to over-produce industrially and pharmaceutically relevant proteins. There are a variety expression systems in terms types transcriptional patterns, among which the auto-inducible growth-phase-dependent promoters gaining increasing favor due their inducer-independent feature, allowing for potential scale-up. To expand applicability system, novel auto-regulatory system...
From peroxynitrite to HOCl: two probes are reported, sharing the same BODIPY core but differing only by a minimized variation in recognition site.
Bacillus subtilis, a Gram-positive organism, has been developed to be an attractive expression platform produce both secreted and cytoplasmic proteins owing its prominent biological characteristics. We previously auto-inducible system containing the srfA promoter (PsrfA) which was activated by signal molecules acting in quorum-sensing pathway for competence. The P exhibited unique property of inducer-free activity that is closely correlated with cell density.To improve PsrfA-mediated...
Bisphenol A (BPA) is an important industrial raw material. Because of its widespread use and increasing release into environment, BPA has become a new environmental pollutant. Previous studies about BPA's effects in plants focus on certain growth stage. However, the plant's response to pollutants varies at different stages. Therefore, this work, soybean roots stages were investigated by determining reactive oxygen species levels, membrane lipid fatty acid composition, peroxidation,...
Nattokinase (NK), which is a member of the subtilisin family, potent fibrinolytic enzyme that might be useful for thrombosis therapy. Extensive work has been done to improve its production food industry. The aim our study was enhance NK by tandem promoters in Bacillus subtilis WB800. Six recombinant strains harboring different plasmids with single promoter (PP43, PHpaII, PBcaprE, PgsiB, PyxiE or PluxS) were constructed, and analysis activity showed PP43 PHpaII exhibited higher expression...
Ratiometric fluorescence/phosphorescence probes for Pt2+ based on the fluorescence quenching from ligand of Salen-type Schiff bases and phosphorescence enhancement resulting Pt(II) complexes have been demonstrated. For enhancement, a good linearity (correlation coefficient R2 = 0.996, n 10) was established with detection limit 11.6 ± 0.2 ppb. The would be decreased to 2.31 0.03 ppb if degassing adopted. To best our knowledge, this value is one most sensitive Pt2+. This system also has...
Abstract Nattokinase (NK) is a serine protease of the subtilisin family; as potent fibrinolytic enzyme, it potentially useful for thrombosis therapy. For NK to be applied an oral medicine treatment cardiovascular diseases, must overcome extremely acidic environments gastrointestinal tract despite its limited stability. In this study, three strategies were adopted improve acid resistance NK: (a) Surface charge engineering, (b) sequence alignment, and (c) mutation based on literature. Eleven...