A5054509896- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Hydrolysis and Bioactive Peptides
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Proteins in Food Systems
- Bacteriophages and microbial interactions
- Animal Genetics and Reproduction
- Microplastics and Plastic Pollution
- biodegradable polymer synthesis and properties
- Microbial Metabolites in Food Biotechnology
- Graphene and Nanomaterials Applications
- Food Chemistry and Fat Analysis
- Heat shock proteins research
- Alcoholism and Thiamine Deficiency
- Food composition and properties
- Analytical Chemistry and Chromatography
- Studies on Chitinases and Chitosanases
- Recycling and Waste Management Techniques
Tianjin University of Science and Technology
2014-2024
Durban University of Technology
2021
Jiangnan University
2010-2013
L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase. Naturally, Escherichia coli does not produce noticeable amounts, but can catabolize it a dehydrogenation reaction mediated FMN-dependent In aims to make E. strain L-lactate, three dehydrogenase genes different bacteria were cloned expressed. The producing strains, 090B1 (B0013-070,...
Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. To improve the efficiency for IMOs, we developed simple and efficient method by using enzyme cocktails composed recombinant Bacillus naganoensis pullulanase produced licheniformis, α-amylase from amyloliquefaciens, barley bran β-amylase, α-transglucosidase Aspergillus niger to perform simultaneous saccharification transglycosylation liquefied starch. After 13 h...
Biodiesel has attracted considerable attention as one of the best choices among alternative and renewable fuels. Large quantities crude glycerol are produced a main co-product with increasing biodiesel production. Currently, problem waste utilization needs to be crucially addressed, not only for environmental protection but also economy industry. In this paper, use production D-lactate by engineered Escherichia coli was investigated. Engineered E. B0013-070 homolactic pathway synthesis...
Lignocellulosic ethanol could offer a sustainable source to meet the increasing worldwide demand for fuel. However, efficient and simultaneous metabolism of all types sugars in lignocellulosic hydrolysates by ethanol-producing strains is still challenge. An engineered strain Escherichia coli B0013-2021HPA with regulated glucose utilization, which use monosaccharides except cell growth production, was constructed. In E. B0013-2021HPA, pta-ackA, ldhA pflB were deleted block formation acetate,...
Escherichia coli strain CICIM B0013 was genetically engineered to efficiently produce optically pure D-lactate (higher than 99.9%) from glycerol with a minimum of by-products. When E. coli B0013-070 (DackA, Dpta, Dpps, DpflB, Ddld, DpoxB, DadhE,DfrdA) was cultivated aerobically for 9 h followed by 27 under microaerobic fermentation, it produced 98.5 g l-1 of no more 2 l-1 total by-products glycerol. During the phase, the average productivity and yield were 3.45 l-1 h-1 and 64 g/100 glycerol,...
Burdock (Arctium lappa L.) is a fructan-rich plant with prebiotic potential. The aim of this study was to develop an efficient enzymatic route prepare fructooligosaccharides (FOS)-rich and highly antioxidative syrup using burdock root as raw material. Endo-inulinase significantly improved the yield FOS 2.4-fold while tannase pretreatment further increased 2.8-fold. Other enzymes, including endo-polygalacturonase, endo-glucanase endo-xylanase, were able increase total soluble sugar by 11.1%...
Efficient production of D-lactate by engineered Escherichia coli entails balancing cell growth and product synthesis. To develop a metabolic switch to implement desirable transition from fermentation, thiamine auxotroph B0013-080A was constructed in highly efficient producer E. strain B0013-070. This achieved inactivation thiE, gene encoding phosphate synthase for biosynthesis monophosphate. The resultant mutant failed grow on the medium absence yet restored when exogenous provided. A linear...
Abstract Ammonium hydroxide is conventionally used as an alkaline reagent and cost-effective nitrogen source in enzyme manufacturing processes. However, few ammonia-inducible expression systems have been described thus far. In this study, genomic-wide transcriptional changes Bacillus licheniformis CBBD302 cultivated media supplemented with ammonia were analyzed, resulting identification of 1443 differently expressed genes, which 859 genes upregulated 584 downregulated. Subsequently, the...
Bacillus licheniformis is a well-known platform strain for production of industrial enzymes. However, the development genetically stable recombinant B. high-yield enzyme still laborious. Here, pair plasmids, pUB-MazF and pUB'-EX1, were firstly constructed. thermosensitive, self-replicable plasmid. It was able to efficiently cure from host cell through induced expression an endoribonuclease MazF, which lethal cell. pUB'-EX1 nonreplicative integrative Its replication dependent on...