A5075879978- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Peptidase Inhibition and Analysis
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Reproductive biology and impacts on aquatic species
- Insect and Pesticide Research
- Mesenchymal stem cell research
- Sperm and Testicular Function
- Aldose Reductase and Taurine
- Insect Resistance and Genetics
- Traditional Chinese Medicine Analysis
- NF-κB Signaling Pathways
- IgG4-Related and Inflammatory Diseases
- Methane Hydrates and Related Phenomena
- Geological and Geophysical Studies
- Bacteriophages and microbial interactions
- Drug Solubulity and Delivery Systems
- Natural Compounds in Disease Treatment
- Occupational exposure and asthma
- Melanoma and MAPK Pathways
- Biological Activity of Diterpenoids and Biflavonoids
- Effects of Radiation Exposure
Shanghai Ocean University
2020-2024
Ministry of Agriculture and Rural Affairs
2024
Liaocheng University
2021-2023
Qingdao Agricultural University
2022
Changsha University
2018-2020
Tianjin University of Science and Technology
2020
Institute of Botany
2017
Chinese Academy of Sciences
2017
Chinese Academy of Medical Sciences & Peking Union Medical College
2013
Zhangqiu City People's Hospital
2013
Abstract Laodelphax striatellus (Fallén) is one of the most destructive pests rice, and has developed high resistance to imidacloprid. Our previous work indicated a strong association between imidacloprid overexpression cytochrome P450 gene CYP6AY3v2 in L. resistant strain (Imid‐R). In this study, transgenic Drosophila melanogaster line that overexpressed was established found confer increased levels resistance. Furthermore, co‐expressed with D. reductase (CPR) Spodoptera frugiperda 9 (SF9)...
Salmonella enterica subspecies serovar abortus equi (S. Abortus equi) is the most common cause of abortion in mares. It has recently been found to donkeys more frequently China. A novel virulent bacteriophage vB_SabS_Sds2 (hereafter designated as Sds2) was isolated from feces using a S. strain host. Phage Sds2 had an isometric polyhedral head and uncontracted long tail, belonging Tequintavirus, Markadamsvirinae, Demerecviridae, Caudovirales. The genome phage 114,770 bp, with GC content...
摘要: 浅部气赋存于浅部成岩强度低的未固结岩石内,其岩石物理特征、地震反射特征都区别于深层已成岩岩石. 为建立浅部气藏地球物理识别技术,笔者利用浅层测井数据分析该盆地浅层岩性、流体性质对岩石速度、密度、纵横波速度比的影响,明确浅部气层敏感弹性参数. 在此基础上,利用颗粒接触岩石物理模型进行浅层岩石物理建模,获得了浅部气层和水层的纵波速度、AVO特征随孔隙度变化模板. 在此基础上,利用地震正演手段查明了浅部气层地震反射特征,明确了浅部气层识别标志,并从地震反射结构特征、反演弹性参数特征、AVO特征入手实现了浅部气层的识别. 首次将Direct Hydrocarbon Identifier by Induced Polarization(DHIP)勘探技术应用于浅部气藏饱和度预测,实现了研究区浅部气藏饱和度定性预测. 该套浅部气藏评价技术是目前琼东南盆地浅层勘探的首次应用,对该盆地后续浅部气藏勘探具有建设性意义. 关键词: 琼东南 / 超深水 浅层 DHIP 岩石物理建模 地震反射特征 含气饱和度预测
A new serine carboxypeptidase gene, capA, was identified in Aspergillus niger CBS 513.88 by reading genomic information and performing sequence alignment, the gene cloned expressed Pichia pastoris GS115. In a shake flask, enzyme activity of recombinant strain GS115 (pPIC9K-capA) reached 209.3 U mg-1. The optimal temperature pH for were determined to be 45 °C 6.0, respectively. After incubation at 40-50 or 4.0-8.0 1 h, retained more than 80% 60% its initial activity. presence 1-10 mmol L-1...
To test the curative effect of human umbilical cord-derived mesenchymal stem cells on rat acute radioactive enteritis and thus to provide clinical therapeutic basis for radiation sickness. Human were cultivated in vitro model rats was established. Then, cord injected into via tail vein. Visual histopathological changes experimental observed. After injection, prevention group treatment had remarkably better survival status than those control group. The histological observations revealed that...
Oligopeptide transporter 1 (Pept1) is located on the brush border membrane of intestinal epithelium and plays an important role in dipeptide tripeptide absorption from protein digestion. In this study, we cloned characterized cDNA sequence Janus kinase 2 (JAK2) Ctenopharyngodon idella. The expression patterns JAK2 various tissues developmental stages were by quantitative real-time PCR (qRT-PCR). mRNA levels Pept1 regulated leptin intestine also analyzed vitro vivo. 3378 bp length, was...
Abstract Taurine transporter (TauT) is a sodium- and chloride-dependent transport carrier, it plays key role in the transportation of taurine. At present, many functions TauT fish remain obscure. In this study, cDNA clone for encoding taurine was isolated from Goldfish Carassius auratus. The nucleotide sequence predicted 625 amino acids with significant homology to gene Zebrafish Danio rerio. Hydropathic analysis revealed stretches hydrophobic that were indicative 12 transmembrane domains...
Coilia nasus is an endangered fish species in the Yangtze River, and there urgent need to know genes related its reproduction fertility. The DAZ family including boule, daz dazl, plays important role germ cells development. In this study, cDNA sequence of boule (Cnbol) dazl (Cndazl) were cloned their expression patterns characterized nasus. RT-PCR showed that Cnbol Cndazl mRNAs was restricted adult gonads. section situ hybridization indicated temporospatial significantly different. testis,...
In recent years, the Chinese hook snout carp (Opsariichthys bidens) is becoming increasingly popular due to its high nutritional value and delicious taste. However, anthropogenic influences have caused a rapid reduction in population. Considering long-term development sustainability, it essential conduct research on reproductive development. this study, germ-cell related genes dazl (Obdazl) dnd (Obdnd) were cloned, their expression patterns revealed. RT-PCR analysis indicated that Obdazl...
摘要: 基于琼东南盆地中央峡谷已有钻井和高品质新地震资料,对第四纪中央峡谷体系的外部形态、内部构成、沉积演化过程及其主控因素等进行了系统梳理和研究,明确中央峡谷体系经历了晚中新世侵蚀充填期(Ⅰ期),早上新世平静充填期(Ⅱ期)和第四纪早期回春充填期(Ⅲ期)3期演化阶段,提出控制中央峡谷体系前两期形成的负地貌和物源供给在第四纪峡谷西段仍然存在.进一步研究表明西段早期多物源体系在第四纪变为昆嵩隆起秋盆河单物源,进积型陆坡控制了半限制型负地貌和砂质沉积逐渐向南东迁移,并在第四纪早期(S14)填平峡谷.在此认识指导下首次在峡谷西段浅层发现了3期第四纪半限制型海底扇群,具备"深浅双源供烃-优势通道复合输导-浅层水合物地层封盖-海底扇成藏"的成藏模式,是下一步深水区浅层寻找大中型气田的有利新领域. 关键词: 中央峡谷体系 / 第四纪 沉积演化 油气前景 琼东南盆地 石油地质
A predicted metalloproteinase gene, HypZn , was cloned from Aspergillus niger CGMCC 3.7193 and expressed in Pichia pastoris GS115, the physicochemical characteristics of recombinant were investigated after separation purification. The results showed that specific activity purified reached 1859.2 U/mg, optimum temperature pH value 35°C 7.0, respectively. remained stable both at 40°C values between 5.0 8.0. preferred substrate soybean protein isolates, K m V max 21.5 μmol/mL 4926.6...
Proteases catalyze the hydrolysis of peptide bonds proteins, thereby improving dietary protein digestibility, nutrient availability, as well flavor and texture fermented food feed products. The lactobacilli Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) Pediococcus acidilactici are widely used in fermentations due to their broad metabolic capabilities safe use. However, extracellular protease activity these two species is low. Here, we optimized expression secretion L. P....