Qiang Pan‐Hammarström
A5061716968- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- DNA Repair Mechanisms
- Cancer Genomics and Diagnostics
- Blood disorders and treatments
- COVID-19 Clinical Research Studies
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Diabetes and associated disorders
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Viral-associated cancers and disorders
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
Karolinska Institutet
2016-2025
Shanghai Institute of Nutrition and Health
2018-2025
University of Chinese Academy of Sciences
2017-2025
Anhui Medical University
2025
Third People's Hospital of Hefei
2025
Center for Excellence in Molecular Cell Science
2015-2024
Radboud University Medical Center
2024
Qingdao Agricultural University
2022-2024
Radboud University Nijmegen
2024
Qingdao Academy of Agricultural Sciences
2024
Circulating autoantibodies (auto-Abs) neutralizing high concentrations (10 ng/mL, in plasma diluted 1 to 10) of IFN-α and/or -ω are found about 10% patients with critical COVID-19 pneumonia, but not subjects asymptomatic infections. We detect auto-Abs 100-fold lower, more physiological, (100 pg/mL, 1/10 dilutions plasma) 13.6% 3,595 COVID-19, including 21% 374 > 80 years, and 6.5% 522 severe COVID-19. These antibodies also detected 18% the 1,124 deceased (aged 20 days-99 years; mean: 70...
Abstract Transcript alterations often result from somatic changes in cancer genomes 1 . Various forms of RNA have been described cancer, including overexpression 2 , altered splicing 3 and gene fusions 4 ; however, it is difficult to attribute these underlying genomic owing heterogeneity among patients tumour types, the relatively small cohorts for whom samples analysed by both transcriptome whole-genome sequencing. Here we present, our knowledge, most comprehensive catalogue...
B-cell survival depends on signals induced by activating factor (BAFF) binding to its receptor (BAFF-R). In mice, mutations in BAFF or BAFF-R cause lymphopenia and antibody deficiency. Analyzing expression BAFF-binding B cells common variable immunodeficiency (CVID) patients, we identified two siblings carrying a homozygous deletion the gene. Removing most of transmembrane part, precludes expression. Without BAFF-R, development is arrested at stage transitional numbers all subsequent stages...
Autosomal inborn errors of type I IFN immunity and autoantibodies against these cytokines underlie at least 10% critical COVID-19 pneumonia cases. We report very rare, biochemically deleterious X-linked TLR7 variants in 16 unrelated male individuals aged 7 to 71 years (mean: 36.7 years) from a cohort 1,202 patients 0.5 99 52.9 with unexplained pneumonia. None the 331 asymptomatically or mildly infected 1.3 102 38.7 tested carry such (p = 3.5 × 10-5). The phenotypes five hemizygous relatives...
Abstract Stat6 is known to drive macrophage M2 polarization. However, how polarization fine-tuned by poorly understood. Here, we find that Lys383 of acetylated the acetyltransferase CREB-binding protein (CBP) during activation suppress Mechanistically, Trim24, a CBP-associated E3 ligase, promotes acetylation catalyzing CBP ubiquitination at Lys119 facilitate recruitment Stat6. Loss Trim24 inhibits and thus in both mouse human macrophages, potentially compromising antitumor immune responses....
Abstract Purpose: Relapsed or refractory diffuse large B-cell lymphoma (rrDLBCL) is fatal in 90% of patients, and yet little known about its biology. Experimental Design: Using exome sequencing, we characterized the mutation profiles 38 rrDLBCL biopsies obtained at time progression after immunochemotherapy. To identify genes that may be associated with relapse, compared frequency samples relapse to an unrelated cohort 138 diagnostic DLBCLs separately amplified specific mutations their...
Monitoring the adaptive immune responses during natural course of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection provides useful information for development vaccination strategies against this virus and its emerging variants. We thus profiled serum anti-SARS-CoV-2 antibody (Ab) levels specific memory B T cell in convalescent coronavirus disease 2019 (COVID-19) patients.
Significance There is growing evidence that preexisting autoantibodies neutralizing type I interferons (IFNs) are strong determinants of life-threatening COVID-19 pneumonia. It important to estimate their quantitative impact on mortality upon SARS-CoV-2 infection, by age and sex, as both the prevalence these risk death increase with higher in men. Using an unvaccinated sample 1,261 deceased patients 34,159 individuals from general population, we found against IFNs strongly increased...
The recent emergence of the Omicron variant has raised concerns on vaccine efficacy and urgent need to study more efficient vaccination strategies. Here we observed that an mRNA booster in individuals vaccinated with two doses inactivated significantly increased plasma level specific antibodies bind receptor-binding domain (RBD) or spike (S) ectodomain (S1 + S2) both G614 variants, compared homologous vaccine. RBD- S-specific IgG virus neutralization titers against variants concern...
Neutralizing antibodies that target the receptor-binding domain (RBD) of SARS-CoV-2 spike protein are among most promising approaches against COVID-191,2. A bispecific IgG1-like molecule (CoV-X2) has been developed on basis C121 and C135, two derived from donors who had recovered COVID-193. Here we show CoV-X2 simultaneously binds independent sites RBD and, unlike its parental antibodies, prevents detectable binding to cellular receptor virus, angiotensin-converting enzyme 2 (ACE2)....
Multisystem inflammatory syndrome in children (MIS-C) is a rare and severe condition that follows benign COVID-19. We report autosomal recessive deficiencies of OAS1 , OAS2 or RNASEL five unrelated with MIS-C. The cytosolic double-stranded RNA (dsRNA)–sensing generate 2′-5′-linked oligoadenylates (2-5A) activate the single-stranded RNA–degrading ribonuclease L (RNase L). Monocytic cell lines primary myeloid cells OAS1, OAS2, RNase produce excessive amounts cytokines upon dsRNA acute...
Recessive or dominant inborn errors of type I interferon (IFN) immunity can underlie critical COVID-19 pneumonia in unvaccinated adults. The risk children, which is much lower than adults, remains unexplained. In an international cohort 112 children (<16 yr old) hospitalized for pneumonia, we report 12 (10.7%) aged 1.5–13 with (7 children), severe (3), and moderate (2) 4 the 15 known clinically recessive biochemically complete IFN immunity: X-linked TLR7 deficiency children) autosomal...
Autoantibodies neutralizing type I interferons (IFNs) can underlie critical COVID-19 pneumonia and yellow fever vaccine disease. We report here on 13 patients harboring autoantibodies IFN-α2 alone (five patients) or with IFN-ω (eight from a cohort of 279 (4.7%) aged 6–73 yr influenza pneumonia. Nine four had antibodies high low concentrations, respectively, IFN-α2, six two IFN-ω. The patients’ increased A virus replication in both A549 cells reconstituted human airway epithelia. prevalence...
The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and immediately classified as a of concern (VOC), since it shows substantially more mutations in spike protein than any previous variant, especially receptor-binding domain (RBD). We analyzed binding RBD to human angiotensin-converting enzyme-2 receptor (ACE2) ability sera from patients or vaccinees comparison Wuhan, Beta, Delta variants.
Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy vaccines and antiviral monoclonal antibodies. Continued development immunotherapies vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions virus spike glycoprotein are both functionally relevant averse change, we identified human neutralizing antibodies highly conserved epitopes....
Abstract Background We previously reported that impaired type I IFN activity, due to inborn errors of TLR3- and TLR7-dependent interferon (IFN) immunity or autoantibodies against IFN, account for 15–20% cases life-threatening COVID-19 in unvaccinated patients. Therefore, the determinants remain be identified ~ 80% cases. Methods report here a genome-wide rare variant burden association analysis 3269 patients with COVID-19, 1373 SARS-CoV-2-infected individuals without pneumonia. Among 928...
The emergence of Omicron lineages and descendent subvariants continues to present a severe threat the effectiveness vaccines therapeutic antibodies. We have previously suggested that an insufficient mucosal immunoglobulin A (IgA) response induced by mRNA is associated with surge in breakthrough infections. Here, we further show intramuscular and/or inactivated cannot sufficiently boost secretory IgA uninfected individuals, particularly against variant. thus engineered characterized...
We found that 19 (10.4%) of 183 unvaccinated children hospitalized for COVID-19 pneumonia had autoantibodies (auto-Abs) neutralizing type I IFNs (IFN-α2 in 10 patients: IFN-α2 only three, plus IFN-ω five, and IFN-α2, IFN-β two; nine patients). Seven (3.8%) Abs at least ng/ml one IFN, whereas the other 12 (6.6%) 100 pg/ml. The auto-Abs neutralized both unglycosylated glycosylated IFNs. also detected pg/ml 4 2,267 uninfected (0.2%) 45 (2%). odds ratios (ORs) life-threatening were, therefore,...