A5009314747- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- Botulinum Toxin and Related Neurological Disorders
- Immune Cell Function and Interaction
- Toxin Mechanisms and Immunotoxins
- Neurological disorders and treatments
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Hereditary Neurological Disorders
- Biosensors and Analytical Detection
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Biochemical and Structural Characterization
- Viral gastroenteritis research and epidemiology
- Immunodeficiency and Autoimmune Disorders
- Bacillus and Francisella bacterial research
Technische Universität Braunschweig
2016-2025
The University of Texas MD Anderson Cancer Center
2023
Biotechnology Institute
2016-2022
Medizinische Hochschule Hannover
2022
The University of Texas Health Science Center at Houston
2017-2021
Memorial Hermann–Texas Medical Center
2021
Karlsruhe Institute of Technology
2010
Leibniz University Hannover
2002-2003
Carl von Ossietzky Universität Oldenburg
1999
Yersinia pseudotuberculosis is a Gram-negative bacterium and zoonotic pathogen responsible for wide range of diseases, ranging from mild diarrhea, enterocolitis, lymphatic adenitis to persistent local inflammation. The Y. invasin D (InvD) molecule belongs the (InvA)-type autotransporter proteins, but its structure function remain unknown. In this study, we present first crystal InvD, analyzed expression in murine infection model, identified target host. We found that InvD induced at 37 °C...
REVIEW article Front. Immunol., 29 July 2013Sec. B Cell Biology Volume 4 - 2013 | https://doi.org/10.3389/fimmu.2013.00217
CD4+CD25highFOXP3+ regulatory T cells (Tregs) are involved in graft-specific tolerance after solid organ transplantation. However, adoptive transfer of polyspecific Tregs alone is insufficient to prevent graft rejection even rodent models, indicating that required. We developed a highly specific chimeric antigen receptor recognizes the HLA molecule A*02 (referred as A2-CAR). Transduction into natural (nTregs) changes specificity nTregs without alteration their phenotype and epigenetic...
Monitoring the adaptive immune responses during natural course of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection provides useful information for development vaccination strategies against this virus and its emerging variants. We thus profiled serum anti-SARS-CoV-2 antibody (Ab) levels specific memory B T cell in convalescent coronavirus disease 2019 (COVID-19) patients.
Adoptive immunotherapy with ex vivo expanded, polyspecific regulatory T cells (Tregs) is a promising treatment for graft-versus-host disease. Animal transplantation models used by us and others have demonstrated that the adoptive transfer of allospecific Tregs offers greater protection from graft rejection than polyclonal Tregs. This finding in contrast to those autoimmune models, where had very limited effects, while antigen-specific were promising. However, autoimmunity cannot be isolated...
The recent emergence of the Omicron variant has raised concerns on vaccine efficacy and urgent need to study more efficient vaccination strategies. Here we observed that an mRNA booster in individuals vaccinated with two doses inactivated significantly increased plasma level specific antibodies bind receptor-binding domain (RBD) or spike (S) ectodomain (S1 + S2) both G614 variants, compared homologous vaccine. RBD- S-specific IgG virus neutralization titers against variants concern...
The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and immediately classified as a of concern (VOC), since it shows substantially more mutations in spike protein than any previous variant, especially receptor-binding domain (RBD). We analyzed binding RBD to human angiotensin-converting enzyme-2 receptor (ACE2) ability sera from patients or vaccinees comparison Wuhan, Beta, Delta variants.
Sepsis-associated encephalopathy (SAE) is a severe and frequent complication of sepsis causing delirium, coma, long-term cognitive dysfunction. We identified microglia C1q complement activation in hippocampal autopsy tissue patients with increased C1q-mediated synaptic pruning murine polymicrobial model. Unbiased transcriptomics isolated derived from septic mice revealed an involvement the innate immune system, activation, up-regulation lysosomal pathways during SAE parallel to neuronal...
The emergence of Omicron lineages and descendent subvariants continues to present a severe threat the effectiveness vaccines therapeutic antibodies. We have previously suggested that an insufficient mucosal immunoglobulin A (IgA) response induced by mRNA is associated with surge in breakthrough infections. Here, we further show intramuscular and/or inactivated cannot sufficiently boost secretory IgA uninfected individuals, particularly against variant. thus engineered characterized...
Abstract Background The demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput antibody generation addressed by optimizing in vitro selection using phage display which moved major bottleneck to production and purification recombinant antibodies an end-user friendly format. Single chain (sc)Fv fragments require additional tags for detection are not as suitable immunoglobulins (Ig)G many...
Antibody phage display is a proven key technology that allows the generation of human antibodies for diagnostics and therapy. From naive antibody gene libraries - in theory against any target can be selected. Here we describe design, construction characterization an optimized library.The HAL9 HAL10, with combined theoretical diversity 1.5×10(10) independent clones, were constructed from 98 healthy donors using improved vectors. In detail, most common phagemids employed are His/Myc tag...
Today, most approved therapeutic antibodies are provided as immunoglobulin G (IgG), whereas small recombinant antibody formats required for in vitro generation and engineering during drug development. Particularly,single chain (sc) fragments like scFv or scFab well suited phage display bacterial expression, but some have been found to lose affinity conversion into IgG.In this study, we compared the influence of format on maturation CD30-specific fragment SH313-F9, with overall objective...
The novel betacoronavirus severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) causes a form of pneumonia disease called coronavirus 2019 (COVID-19). To develop human neutralizing anti-SARS-CoV-2 antibodies, antibody gene libraries from convalescent COVID-19 patients were constructed and recombinant fragments (scFv) against the receptor-binding domain (RBD) spike protein selected by phage display. STE90-C11 shows subnanometer IC
Abstract COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, new recently emerged sarbecovirus. This virus uses the human ACE2 enzyme as receptor for cell entry, recognizing it with binding domain (RBD) of S1 subunit viral spike protein. We present use phage display to select anti-SARS-CoV-2 antibodies from naïve antibody gene libraries HAL9/10 and subsequent identification 309 unique fully against S1. 17 are RBD, showing inhibition cells expressing scFv-Fc neutralize active...
With six approved products and more than 60 candidates in clinical testing, human monoclonal antibody discovery by phage display is well established as a robust reliable source for the generation of therapeutic antibodies. While vast diversity library philosophies selection strategies have been conceived, power molecular design offered controlling vitro step still to be recognized broader audience outside engineering community. Here, we summarize some opportunities achievements, e.g.,...
Abstract Diphtheria is an infectious disease caused by Corynebacterium diphtheriae . The bacterium primarily infects the throat and upper airways produced diphtheria toxin (DT), which binds to elongation factor 2 blocks protein synthesis, can spread through bloodstream affect organs, such as heart kidneys. For more than 125 years, therapy against has been based on polyclonal horse sera directed DT (diphtheria antitoxin; DAT). Animal have many disadvantages including serum sickness,...