A5002780892- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Vaccine Coverage and Hesitancy
- Monoclonal and Polyclonal Antibodies Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Respiratory Health Research
- Vector-borne infectious diseases
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Pneumonia and Respiratory Infections
- vaccines and immunoinformatics approaches
- Complement system in diseases
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Bacillus and Francisella bacterial research
- Infection Control and Ventilation
- Sarcoidosis and Beryllium Toxicity Research
- Transplantation: Methods and Outcomes
- Pneumocystis jirovecii pneumonia detection and treatment
Helmholtz Centre for Infection Research
2020-2024
Center for Experimental and Clinical Infection Research
2020-2024
Medizinische Hochschule Hannover
2020-2024
Fraunhofer Institute for Interfacial Engineering and Biotechnology
2024
Natural and Medical Sciences Institute
2022
University of Tübingen
2022
Deutsche Post (Germany)
2022
German Center for Infection Research
2022
University College Dublin
2011-2017
National Children’s Research Centre
2013
SARS-CoV-2 is evolving with mutations in the receptor binding domain (RBD) being of particular concern. It important to know how much cross-protection offered between strains following vaccination or infection. Here, we obtain serum and saliva samples from groups vaccinated (Pfizer BNT-162b2), infected uninfected individuals characterize antibody response RBD mutant strains. Vaccinated have a robust humoral after second dose high IgG titers saliva. Antibody responses however show...
The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and immediately classified as a of concern (VOC), since it shows substantially more mutations in spike protein than any previous variant, especially receptor-binding domain (RBD). We analyzed binding RBD to human angiotensin-converting enzyme-2 receptor (ACE2) ability sera from patients or vaccinees comparison Wuhan, Beta, Delta variants.
Abstract The humoral immune response to SARS-CoV-2 is a benchmark for immunity and detailed analysis required understand the manifestation progression of COVID-19, monitor seroconversion within general population, support vaccine development. majority currently available commercial serological assays only quantify antibody against individual antigens, limiting our understanding response. To overcome this, we have developed multiplex immunoassay (MultiCoV-Ab) including spike nucleocapsid...
BackgroundPatients with chronic renal insufficiency on maintenance haemodialysis face an increased risk of COVID-19 induced mortality and impaired vaccine responses. To date, only a few studies have addressed SARS-CoV-2 elicited immunity in this immunocompromised population.MethodsWe assessed immunogenicity the mRNA BNT162b2 at-risk dialysis patients characterised systemic cellular humoral immune responses serum saliva using interferon γ release assay multiplex-based cytokine immunoglobulin...
Article27 April 2021Open Access Transparent process NeutrobodyPlex—monitoring SARS-CoV-2 neutralizing immune responses using nanobodies Teresa R Wagner orcid.org/0000-0002-1050-5948 Pharmaceutical Biotechnology, Eberhard Karls University, Tuebingen, Germany Natural and Medical Sciences Institute, University of Reutlingen, Search for more papers by this author Elena Ostertag Interfaculty Institute Biochemistry, Philipp D Kaiser Marius Gramlich Natalia Ruetalo Virology Epidemiology Viral...
Influenza A virus (IAV), a major airborne pathogen, is closely associated with significant morbidity and mortality. The primary target for influenza replication the respiratory epithelium, which reacts to infection by mounting multifaceted antiviral response. part of this mucosal host defense generation reactive oxygen species (ROS) NADPH oxidases. Duox1 Duox2 are main ROS-producing enzymes in airway but their contribution mammalian still ill defined.To gain better understanding Duox...
Until now, information on the spread of SARS-CoV-2 infections in Germany has been based mainly data from public health offices. It may be assumed that these do not include many cases asymptomatic and mild infection.
Abstract SARS-CoV-2 variants accumulating immune escape mutations provide a significant risk to vaccine-induced protection against infection. The novel variant of concern (VoC) Omicron BA.1 and its sub-lineages have the largest number amino acid alterations in Spike protein date. Thus, they may efficiently recognition by neutralizing antibodies, allowing breakthrough infections convalescent vaccinated individuals particular those who only received primary immunization scheme. We analyzed...
Abstract Patients undergoing chronic hemodialysis were among the first to receive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccinations because of their increased risk for disease and high case-fatality rates. By using a previously reported cohort from Germany at-risk patients healthy donors, where antibody responses examined 3 weeks after second vaccination, we assessed systemic cellular humoral immune in serum saliva 4 months vaccination with Pfizer-BioNTech BNT162b2...
Abstract The SARS-CoV-2 pandemic calls for the rapid development of diagnostic, preventive, and therapeutic approaches. CD4 + CD8 T cell-mediated immunity is central control protection from viral infections [1-3] . A prerequisite to characterize T-cell immunity, but also vaccines immunotherapies, identification exact epitopes presented on human leukocyte antigens (HLA) [2-8] This first work identifying characterizing SARS-CoV-2-specific cross-reactive HLA class I HLA-DR in convalescents (n =...
Haemodialysis patients respond poorly to vaccination and continue be at-risk for severe COVID-19. Therefore, dialysis were among the first which a fourth COVID-19 was recommended. However, targeted information on how best maintain immune protection after SARS-CoV-2 vaccinations in groups remains limited. We provide, of our knowledge, time longitudinal response data controls triple BNT162b2 latter subsequent full-dose mRNA-1273. analysed systemic mucosal humoral IgG responses against...
Abstract The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and classified as a of concern (VOC). shows substantially more mutations in spike protein than any previous variant, mostly receptor binding domain (RBD). We analyzed RBD to human ACE2 (hACE2) ability sera from patients or vaccinees comparison Wuhan, Beta Delta RBDs variants. All were produced insect cells. hACE2 ELISA microscale thermophoresis (MST). Similarly, 27...
Sarcoidosis is a multi-systemic disorder of unknown etiology, characterized by the presence non-caseating granulomas in target organs. In 90% cases, there thoracic involvement. Fifty to seventy percent pulmonary sarcoidosis patients will experience acute, self-limiting disease. For subgroup who develop persistent disease, no targeted therapy currently available.To investigate potential single nucleotide polymorphism (SNP), Toll-like receptor 3 Leu412Phe (TLR3 L412F; rs3775291), as causative...
Recent increases in SARS-CoV-2 infections have led to questions about duration and quality of vaccine-induced immune protection. While numerous studies been published on responses triggered by vaccination, these often focus studying the impact one or two immunisation schemes within subpopulations such as immunocompromised individuals healthcare workers. To provide information against SARS-CoV-2, we analyzed antibody titres various antigens ACE2 binding inhibition wild-type variants concern...
Abstract Prevalence of SARS-CoV-2 antibodies is an essential indicator to guide measures. Few population-based estimates are available in Germany. We determine seroprevalence allowing comparison between regions, time points, socio-demographic and health-related factors. MuSPAD a sequential multi-local study. randomly recruited adults five counties with differing cumulative incidence July 2020 - February 2021. Serostatus was determined using Spike S1-specific IgG ELISA. county-wise...
The rapid emergence of the Omicron variant and its large number mutations led to classification as a concern (VOC) by World Health Organization. Subsequently, evolved into distinct sublineages (eg, BA.1 BA.2), which currently represent majority global infections. Initial studies neutralizing response toward in convalescent vaccinated individuals showed substantial reduction.We assessed antibody (immunoglobulin G [IgG]) binding, ACE2 (angiotensin-converting enzyme 2) binding inhibition, IgG...
Abstract Given the importance of humoral immune response to SARS-CoV-2 as a global benchmark for immunity, detailed analysis is needed monitor seroconversion in general population, understand manifestation and progression COVID-19 disease, ultimately predict outcome vaccine development. In contrast currently available serological assays, which are only able resolve antibody on an individual antigen level, we developed multiplex immunoassay, included spike nucleocapsid proteins endemic human...
Abstract SARS-CoV-2 variants accumulating immune escape mutations provide a significant risk to vaccine-induced protection. The novel variant of concern (VoC) Omicron (B.1.1.529) has the largest number amino acid alterations in its Spike protein date. Thus, it may efficiently recognition by neutralizing antibodies, allowing breakthrough infections convalescent and vaccinated individuals. We analyzed neutralization activity sera from individuals after vaccination with all mRNA-, vector- or...
Lyme borreliosis (LB) is the most common tick-borne infectious disease in northern hemisphere. The diagnosis of LB usually made by clinical symptoms and subsequently supported serology. In Europe, a two-step testing consisting an enzyme-linked immunosorbent assay (ELISA) immunoblot recommended. However, due to low sensitivity currently available tests, antibody detection sometimes inaccurate, especially early phase infection, leading underdiagnoses.To improve upon Borrelia diagnostics, we...
Prevalence of SARS-CoV-2 antibodies is an essential indicator to guide measures. Few population-based estimates are available in Germany. We determine seroprevalence allowing comparison between regions, time points, socio-demographic and health-related factors.MuSPAD a sequential multi-local study. randomly recruited adults five counties with differing cumulative incidence July 2020 - February 2021. Serostatus was determined using Spike S1-specific IgG ELISA. county-wise proportions...
Abstract Background Patients with chronic renal insufficiency on intermittent hemodialysis face an increased risk of COVID-19 induced mortality and impaired vaccine responses. To date, only few studies addressed SARS-CoV-2 elicited immunity in this immunocompromised population. Methods We assessed immunogenicity the mRNA BNT162b2 at dialysis patients characterized systemic cellular humoral immune responses serum saliva using interferon γ release assay multiplex-based cytokine immunoglobulin...