A5088161106- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- COVID-19 Clinical Research Studies
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Protein Degradation and Inhibitors
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- Multiple Myeloma Research and Treatments
- Chronic Lymphocytic Leukemia Research
- Chemokine receptors and signaling
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- SARS-CoV-2 detection and testing
- Hepatitis B Virus Studies
- Cancer Genomics and Diagnostics
- COVID-19 and healthcare impacts
- Long-Term Effects of COVID-19
- Advanced biosensing and bioanalysis techniques
- Immunodeficiency and Autoimmune Disorders
- Influenza Virus Research Studies
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
University of Tübingen
2016-2025
German Cancer Research Center
2019-2025
Deutschen Konsortium für Translationale Krebsforschung
2019-2025
Institute of Immunology
2023-2024
Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology
2020-2023
University Children's Hospital Tübingen
2016-2023
Robert Bosch (Germany)
2020-2023
Heidelberg University
2020-2023
Institut Paoli-Calmettes
2021-2023
National Institutes of Health
2020-2022
Abstract T cell immunity is central for the control of viral infections. CoVac-1 a peptide-based vaccine candidate, composed SARS-CoV-2 epitopes derived from various proteins 1,2 , combined with Toll-like receptor 1/2 agonist XS15 emulsified in Montanide ISA51 VG, aiming to induce profound combat COVID-19. Here we conducted phase I open-label trial, recruiting 36 participants aged 18–80 years, who received single subcutaneous vaccination. The primary end point was safety analysed until day...
Background The human leucocyte antigen (HLA) complex controls adaptive immunity by presenting defined fractions of the intracellular and extracellular protein content to immune cells. Understanding benign HLA ligand repertoire is a prerequisite define safe T-cell-based immunotherapies against cancer. Due poor availability tissues, if available, normal tissue adjacent tumor has been used as surrogate when defining tumor-associated antigens. However, this comparison proven be insufficient even...
Longitudinal analysis identifies SARS-CoV-2 peptide targets of durable memory T cell responses in COVID-19 convalescent individuals.
Immune-checkpoint inhibitors (ICI) have transformed oncological therapy. Up to 20% of all non-small cell lung cancers (NSCLCs) show durable responses upon treatment with ICI, however, robust markers predict therapy response are missing. Here we that blood platelets interact cancer cells and PD-L1 protein is transferred from tumor in a fibronectin 1, integrin α5β1 GPIbα-dependent manner. Platelets NSCLC patients found express platelet possess the ability inhibit CD4 CD8 T-cells. An algorithm...
Microbial organisms have key roles in numerous physiological processes the human body and recently been shown to modify response immune checkpoint inhibitors
The high throughput analysis of proteins with mass spectrometry (MS) is highly valuable for understanding human biology, discovering disease biomarkers, identifying therapeutic targets, and exploring pathogen interactions. To achieve these goals, specialized proteomics subfields, including plasma proteomics, immunopeptidomics, metaproteomics, must tackle specific analytical challenges, such as an increased identification ambiguity compared to routine experiments. Technical advancements in MS...
Significance Despite the revolution in cancer therapy initiated by checkpoint inhibitors, durable clinical responses remain sporadic many types of cancer, including ovarian cancer. Understanding which antigens are essentially presented tumor cells and further able to be recognized T provides a major step toward novel effective targeted immunotherapies. In this study, we comprehensively analyzed immunopeptidomic landscape carcinoma compared it variety benign sources identify exclusively on...
Platelets promote metastasis, among others by coating cancer cells traveling through the blood, which results in protection from NK cell immune-surveillance. The underlying mechanisms, however, remain to be fully elucidated. Here we report that platelet-coating reduces surface expression of NKG2D ligands, particular MICA and MICB, on tumor cells, was mirrored enhanced release their soluble ectodomains. Similar were obtained upon exposure platelet-releasate can attributed sheddases ADAM10...
Abstract The humoral immune response to SARS-CoV-2 is a benchmark for immunity and detailed analysis required understand the manifestation progression of COVID-19, monitor seroconversion within general population, support vaccine development. majority currently available commercial serological assays only quantify antibody against individual antigens, limiting our understanding response. To overcome this, we have developed multiplex immunoassay (MultiCoV-Ab) including spike nucleocapsid...
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important track COVID-19 cases and the humoral response infections immunization future vaccines. The aim of this work was develop a simple chromogenic magnetic bead-based immunoassay which allows rapid, inexpensive, quantitative detection human antibodies against serum, plasma, or blood. Recombinant 6xHis-tagged Nucleocapsid protein mobilized on surface Ni2+ beads challenged with serum blood samples obtained from...
Several COVID-19 vaccines are approved to prevent severe disease outcome after SARS-CoV-2 infection. Whereas induction and functionality of antiviral antibody response largely studied, the T cells upon vaccination with different is less studied. Here, we report on cell immunity 4 weeks 6 months regimens an additional booster in comparison responses convalescents prepandemic donors using interferon-gamma ELISpot assays flow cytometry. Increased cross-recognition B.1.1.529 Omicron...
Article27 April 2021Open Access Transparent process NeutrobodyPlex—monitoring SARS-CoV-2 neutralizing immune responses using nanobodies Teresa R Wagner orcid.org/0000-0002-1050-5948 Pharmaceutical Biotechnology, Eberhard Karls University, Tuebingen, Germany Natural and Medical Sciences Institute, University of Reutlingen, Search for more papers by this author Elena Ostertag Interfaculty Institute Biochemistry, Philipp D Kaiser Marius Gramlich Natalia Ruetalo Virology Epidemiology Viral...
Immunotherapies targeting cancer-specific neoantigens have revolutionized the treatment of cancer patients. Recent evidence suggests that epigenetic therapies synergize with immunotherapies, mediated by de-repression endogenous retroviral element (ERV)-encoded promoters, and initiation transcription. Here, we use deep RNA sequencing from cell lines treated DNA methyltransferase inhibitor (DNMTi) and/or Histone deacetylase (HDACi), to assemble a de novo transcriptome identify several thousand...
Abstract The DNAJB1-PRKACA fusion transcript is the oncogenic driver in fibrolamellar hepatocellular carcinoma, a lethal disease lacking specific therapies. This study reports on identification, characterization, and immunotherapeutic application of HLA-presented neoantigens for carcinoma. DNAJB1-PRKACA-derived HLA class I II ligands induce multifunctional cytotoxic CD8 + T-helper 1 CD4 T cells, their cellular processing presentation expressing tumor cells demonstrated by mass...
The development of ribosomal profiling (Riboseq) revealed the immense coding capacity human and viral genomes. Here, we used Riboseq to delineate translatome HIV-1 in infected CD4+ T cells. In addition canonical protein sequences (CDSs), identify 98 alternative open reading frames (ARFs), corresponding small Open Reading Frames (sORFs) that are distributed across HIV genome including UTR regions. Using a database genomes, observe most ARF amino-acid likely conserved among clade B C HIV-1,...
<h3>Background</h3> We previously showed that the bacterial lipopeptide Pam<sub>3</sub>Cys-Ser-Ser, meanwhile established as a toll-like receptor (TLR) 1/2 ligand, acts strong adjuvant for induction of virus specific CD8<sup>+</sup> T cells in mice, when covalently coupled to synthetic peptide. <h3>Case presentation</h3> now designed new water-soluble Pam<sub>3</sub>Cys-derivative, named XS15 and characterized it vitro by TLR2 NF-κB luciferase reporter assay. Further, capacity activate...
Abstract T cell recognition of human leukocyte antigen (HLA)-presented tumor-associated peptides is central for cancer immune surveillance. Mass spectrometry (MS)-based immunopeptidomics represents the only unbiased method direct identification and characterization naturally presented peptides, a key prerequisite development cell-based immunotherapies. This study reports on implementation ion mobility separation-based time-of-flight (TOF IMS ) MS next-generation immunopeptidomics, enabling...