A5053013984- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Plant Pathogenic Bacteria Studies
- Enzyme Catalysis and Immobilization
- Plant nutrient uptake and metabolism
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Metalloenzymes and iron-sulfur proteins
- Biofuel production and bioconversion
- Wastewater Treatment and Nitrogen Removal
- Microbial Fuel Cells and Bioremediation
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Nematode management and characterization studies
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Plant Diversity and Evolution
- Biochemical and Molecular Research
- Protist diversity and phylogeny
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
Universidade Federal do Paraná
2016-2025
Universidade do Extremo Sul Catarinense
2017-2021
Brazilian Biosciences National Laboratory
2021
Brazilian Center for Research in Energy and Materials
2021
Centro Universitário do Espírito Santo
2021
University of Minnesota Rochester
2017
Instituto de Biologia Molecular do Paraná
2016
Centro Universitário Curitiba
2016
National Institute of Standards and Technology
2011
University of Georgia
2010
Summary Nitrogen‐fixing rhizobacteria can promote plant growth; however, it is controversial whether biological nitrogen fixation ( BNF ) from associative interaction contributes to growth promotion. The roots of Setaria viridis , a model C 4 grass, were effectively colonized by bacterial inoculants resulting in significant enhancement growth. Nitrogen‐13 tracer studies provided direct evidence for uptake the host and incorporation into protein. Indeed, plants showed robust under...
The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable colonizing intercellular spaces grasses such as rice sugar cane. genome H. strain SmR1 was sequenced annotated by Paraná State Genome Programme—GENOPAR. composed a circular chromosome 5,513,887 bp contains total 4,804 genes. sequence revealed that highly versatile microorganism with capacity...
The Brazilian Atlantic Forest is one of the 25 biodiversity hot spots in world. Although diversity its fauna and flora has been studied fairly well, little known microbial communities. In this work, we analyzed ecosystem to determine bacterial biodiversity, using 16S rRNA gene sequencing, correlated changes deduced taxonomic profiles with physicochemical characteristics soil. DNAs were purified from soil samples, was amplified construct libraries. Comparison 754 independent sequences 10...
Abstract Background Metagenomics, the application of molecular genomics to consortia non-cultivated microbes, has potential have a substantial impact on search for novel industrial enzymes such as esterases (carboxyl ester hydrolases, EC 3.1.1.1) and lipases (triacylglycerol lipases, 3.1.1.3). In current work, lipase gene was identified from fosmid metagenomic library constructed with "prokaryotic-enriched" DNA fat-contaminated soil collected wastewater treatment plant. Results preliminary...
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pH is frequently reported as the main driver for prokaryotic community structure in soils. However, changes are also linked to "spillover effects" on other chemical parameters (e.g., availability of Al, Fe, Mn, Zn, and Cu) plant growth, but these indirect effects microbial communities rarely investigated. Usually, co-varies with some confounding factors, such land use, soil management tillage inputs), cover, and/or edapho-climatic conditions. So, a more comprehensive analysis direct brings...
The rapid growth of the world's population demands an increase in food production that no longer can be reached by increasing amounts nitrogenous fertilizers. Plant promoting bacteria (PGPB) might alternative to use efficiency (NUE) important crops such wheat. Azospirillum brasilense is one most promising PGPB and wheat roots colonized A. a good model investigate molecular basis plant-PGPB interaction including improvement plant-NUE promoted PGPB.We performed dual RNA-Seq transcriptional...
Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation diazotrophic bacteria. We examined diversity endophytic internal stems and leaves, using molecular biochemical methods. Potato-agar medium was used to cultivate endophytes; 32 isolates were selected for analysis. DNA extracted 16S rRNA gene partially sequenced identification. Gram staining, catalase oxidase tests, API-20E system characterize isolates. The...
Azospirillum sp. strain Sp245 T , originally identified as belonging to brasilense is recognized a plant-growth-promoting rhizobacterium due its ability fix atmospheric nitrogen and produce plant-beneficial compounds. other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly rods, motile with polar lateral flagella. Their growth temperature varies between 20 38 °C their carbon source utilization similar species. A...
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important track COVID-19 cases and the humoral response infections immunization future vaccines. The aim of this work was develop a simple chromogenic magnetic bead-based immunoassay which allows rapid, inexpensive, quantitative detection human antibodies against serum, plasma, or blood. Recombinant 6xHis-tagged Nucleocapsid protein mobilized on surface Ni2+ beads challenged with serum blood samples obtained from...
The interactions between maize, sorghum, wheat and rice plants Herbaspirillum seropedicae were examined microscopically following inoculation with the H. LR15 strain, a Nif+ (Pnif::gusA) mutant obtained by insertion of gusA-kanamycin cassette into nifH gene wild-type strain. expression Pnif::gusA fusion was followed during association diazotroph gramineous species. Histochemical analysis seedlings grown in vermiculite showed that strain colonized root surfaces inner tissues. In early steps...
Abstract Background Rice ( Oryza sativa L.) germplasm represents an extraordinary source of genes that control traits agronomic importance such as drought tolerance. This diversity is the basis for development new cultivars better adapted to water restriction conditions, in particular upland rice, which grown under rainfall. The analyses subtractive cDNA libraries and differential protein expression tolerant susceptible genotypes can contribute understanding genetic use efficiency rice....
In this study we disrupted two Herbaspirillum seropedicae genes, rfbB and rfbC, responsible for rhamnose biosynthesis its incoporation into LPS. GC-MS analysis of the H. wild-type strain LPS oligosaccharide chain showed that rhamnose, glucose N-acetyl glucosamine are predominant monosaccharides, whereas were not found in rfbC strains. The electrophoretic pattern mutants was drastically altered when compared with wild type. Knockout or increased sensitivity towards SDS, polymyxin B sulfate...
Eight virulence factors associated with uropathogenic Escherichia coli (UPEC) were investigated in 204 clinical isolates of E. recovered from urine cultures at counts ≥10 5 .The bacteria classified into two groups according to the number leukocytes samples which they isolated: group I ≤8 leukocytes/ hpf, 104 strains; II >8 leukocytes/hpf, 100 strains.Two multiplex PCR systems used detect genes encoding adhesin P (pap), S (sfa), afimbrial (afa), siderophore aerobactin (aer), alpha-hemolysin...
Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding mechanisms controlling establishment N2-fixing associations developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction...
Plant-bacteria associations have been extensively studied for their potential in increasing crop productivity a sustainable manner. Serratia marcescens is species of Enterobacteriaceae found wide range environments, including soil.Here we describe the genome sequencing and assessment plant growth-promoting abilities S. UENF-22GI, strain isolated from mature cattle manure vermicompost. In vitro, UENF-22GI able to solubilize P Zn, produce indole compounds (likely IAA), colonize hyphae counter...
Background Podocarpus lambertii (Podocarpaceae) is a native conifer from the Brazilian Atlantic Forest Biome, which considered one of 25 biodiversity hotspots in world. The advancement next-generation sequencing technologies has enabled rapid acquisition whole chloroplast (cp) genome sequences at low cost. Several studies have proven potential cp genomes as tools to understand enigmatic and basal phylogenetic relationships different taxonomic levels, well further probe structural functional...
Background Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing protocols for conifer species have based mainly on long-range PCR, which known to be time-consuming difficult implement. Methodology/Principal Findings We developed protocol cpDNA using three...
Abstract Despite the development in DNA sequencing technology, improving number and length of reads, process reconstruction complete genome sequences, so called assembly, is still complex. Only 13% prokaryotic projects have been completed. Draft sequences deposited public databases are fragmented contigs may lack full gene complement. The aim present work to identify assembly errors improve bacterial genomes. biological patterns observed genomic application a priori information can allow...