A5069719164- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Polysaccharides and Plant Cell Walls
- Fungal Infections and Studies
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Nail Diseases and Treatments
- Antifungal resistance and susceptibility
- Heat shock proteins research
- Antibiotic Resistance in Bacteria
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolism and Applications
- Vibrio bacteria research studies
- Studies on Chitinases and Chitosanases
- Plant Pathogens and Fungal Diseases
- Antimicrobial Peptides and Activities
- Agricultural and Food Sciences
- Plant-Microbe Interactions and Immunity
- Protein Structure and Dynamics
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Phytochemistry Medicinal Plant Applications
- Catalysis for Biomass Conversion
Universidade Estadual do Oeste do Paraná
2014-2025
Universidade de São Paulo
2000-2024
Universidade Estadual do Paraná
2020-2023
Zero to Three
2003
Universidade Estadual de Maringá
1997
Universidade Estadual Paulista (Unesp)
1995
The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable colonizing intercellular spaces grasses such as rice sugar cane. genome H. strain SmR1 was sequenced annotated by Paraná State Genome Programme—GENOPAR. composed a circular chromosome 5,513,887 bp contains total 4,804 genes. sequence revealed that highly versatile microorganism with capacity...
We studied the expression of DnaK and GroEL in Acinetobacter baumannii cells (strains ATCC 19606 RS4) under stress caused by heat shock or antibiotics. A Western blot assay showed that levels increased transiently more than 2-fold after exposure bacterial to for 20 min at 50 degrees C. Heat induction was blocked completely when an inhibitor transcription, rifampicin, added 1 before a temperature upshift C, suggesting these chaperones depends on transcription. A. pretreated 45 C 30 were...
The world practice of using agrochemicals for long periods, in an indiscriminated and abusive way, has been a concern the authorities involved public health sustainability natural resources, as consequence environmental contamination. Agrochemicals refer to broad range insecticides, fungicides herbicides, among them stands out atrazine, herbicide intensively used sugarcane, corn sorghum cultures, others. Researches have demonstrated that atrazine toxic effects algae, aquatic plants, insects,...
Abstract The filamentous Thermoascus aurantiacus fungus characterized by its thermophilic nature, is recognized as an exceptional producer of various enzymes with biotechnological applications. This study aimed to explore applications using polygalacturonase (PG) derived from the PI3S3 strain. PG production was achieved through submerged fermentation and subsequent purification via ion-exchange chromatography gel filtration methods. crude extract exhibited a diverse spectrum enzymatic...
ABSTRACT Wickerhamomyces anomalus is a yeast-producing mycocins and has antimicrobial action. Escherichia coli the predominant bacterium of coliforms group; its presence in water indicates fecal contamination, being used as an indicator microbiological analyses. The immobilization cells substances demonstrates great potential for biotechnological applications. This work aimed to assess activity free immobilized mycocins, obtained from W. anomalus, against E. strains coliforms. were different...
β-Xylosidases (EC 3.2.1.37) are among the principal glycosyl hydrolases involved in breakdown of hemicelluloses, catalyzing reduction xylooligosaccharides to free xylose. All GH39 β-xylosidases structurally characterized date display a modular multi-domain organization that assembles tetrameric quaternary structure. In this work, crystal structure and SAXS molecular envelope new β-xylosidase from Caulobacter crescentus (CcXynB2) have been determined. Interestingly, CcXynB2 is monomer...
A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues performed. Using statistical approach as a strategy for optimization, induction activity evaluated residual corn straw, improved so that optimum condition achieved high activities 1003U/mL. According our known, this study is first show levels induction. In addition, application an experimental design with microorganism induce has not...
The aim of this work was to characterize and apply a polygalacturonase Penicillium janthinellum new strain VI2R3M. obtained from P. VI2R3M incubated in cultures passion fruit peel partially purified by ion‐exchange chromatography gel filtration. enzyme showed relative molecular mass 102·0 kDa, maximum activity at pH 5·0, temperature 50°C, 100% stablity 50°C 80% 3·0–5·0. apparent Km, Vmax Kcat values for hydrolyzing polygalacturonic acid were 2·56 mg ml−1, 163·1 U mg−1 277 s−1 respectively....
Staphylococcus aureus is among the major pathogens involved in food poisoning, and meat contaminated with S. coagulase positive considered a public health risk because bacterium resistant to several conventional antimicrobials. Mycocins are substances produced by yeasts that secrete glycoproteins, which can also be called toxin killers, they have an inhibitory action on other microorganisms. The Wickerhamomyces anomalus, one of microorganisms capable producing these mycocins, resulting...
Expression of heat shock genes in Gram-negative proteobacteria is positively modulated by the transcriptional regulator RpoH, sigma(32) subunit RNA polymerase (RNAP). In this study we investigated chaperones DnaK/DnaJ and GroES/GroEL as possible modulators response Caulobacter crescentus. We have shown that cells overexpressing DnaK show poor induction protein (HSP) synthesis, even though levels present a normal transient increase upon stress. On other hand, depletion led to higher increased...