A5072298339- Malaria Research and Control
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Complement system in diseases
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Vector-borne infectious diseases
- Drug Transport and Resistance Mechanisms
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Computational Drug Discovery Methods
- Hepatitis B Virus Studies
- Parasites and Host Interactions
- Animal Virus Infections Studies
- Invertebrate Immune Response Mechanisms
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Virology and Viral Diseases
- interferon and immune responses
- Immune cells in cancer
- Drug-Induced Hepatotoxicity and Protection
Agency for Science, Technology and Research
2016-2025
Nanyang Technological University
2015-2025
Singapore Immunology Network
2015-2024
Université Paris Cité
2006-2024
Inserm
2005-2024
Institut des Maladies Génétiques Imagine
2020-2024
Rockefeller University
2020-2024
Assistance Publique – Hôpitaux de Paris
2020-2024
Howard Hughes Medical Institute
2020-2024
Human Genetic of Infectious Diseases
2024
Recent reports of increased tolerance to artemisinin derivatives--the most recently adopted class antimalarials--have prompted a need for new treatments. The spirotetrahydro-beta-carbolines, or spiroindolones, are potent drugs that kill the blood stages Plasmodium falciparum and vivax clinical isolates at low nanomolar concentration. Spiroindolones rapidly inhibit protein synthesis in P. falciparum, an effect is ablated parasites bearing nonsynonymous mutations gene encoding P-type...
Mouse and human dendritic cells (DCs) are composed of functionally specialized subsets, but precise interspecies correlation is currently incomplete. Here, we showed that murine lung gut lamina propria CD11b+ DC populations were comprised two subsets: FLT3- IRF4-dependent CD24+CD64− DCs contaminating CSF-1R-dependent CD24−CD64+ macrophages. Functionally, loss CD24+CD11b+ abrogated CD4+ T cell-mediated interleukin-17 (IL-17) production in steady state after Aspergillus fumigatus challenge....
Langerhans cells (LCs) are the dendritic (DCs) of epidermis, forming one first hematopoietic lines defense against skin pathogens. In contrast to other DCs, LCs arise from precursors that seed before birth. However, origin these embryonic remains unclear. Using in vivo lineage tracing, we identify a wave yolk sac (YS)-derived primitive myeloid progenitors onset fetal liver hematopoiesis. YS migrate embryo proper, including prospective skin, where they give rise LC precursors, and brain...
Dendritic cell (DC)-mediated cross-presentation of exogenous antigens acquired in the periphery is critical for initiation CD8(+) T responses. Several DC subsets are described human tissues but migratory cross-presenting DCs have not been isolated, despite their potential importance immunity to pathogens, vaccines, and tumors tolerance self. Here, we identified a CD141(hi) present interstitial dermis, liver, lung that was distinct from majority CD1c(+) CD14(+) tissue superior at soluble...
An effective vaccine for malaria is urgently needed. Naturally acquired immunity to develops slowly, and induction of protection in humans can be achieved artificially by the inoculation radiation-attenuated sporozoites means more than 1000 infective mosquito bites.
Dendritic cells (DC) are professional antigen-presenting that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) cytometry by time-of-flight (CyTOF)-to identify blood CD123+CD33+CD45RA+ precursors (pre-DC). Pre-DC share surface markers with plasmacytoid (pDC) but have...
Abstract Given the ongoing SARS-CoV-2 pandemic, identification of immunogenic targets against coronavirus spike glycoprotein will provide crucial advances towards development sensitive diagnostic tools and potential vaccine candidate targets. In this study, using pools overlapping linear B-cell peptides, we report two IgG immunodominant regions on that are recognised by sera from COVID-19 convalescent patients. Notably, one is specific to SARS-CoV-2, which located in close proximity receptor...
Complicated abdominal aortic aneurysm (AAA) is a major cause of mortality in elderly men. Ang II–dependent TGF-β activity promotes progression experimental Marfan syndrome. However, the role models AAA has not been comprehensively assessed. Here, we show that systemic neutralization breaks resistance normocholesterolemic C57BL/6 mice to II–induced formation and markedly increases their susceptibility disease. These aneurysms displayed large spectrum complications on echography, including...
Studies have found different waning rates of neutralising antibodies compared with binding against SARS-CoV-2. The impact antibody rate at the individual patient level on longevity immunity remains unknown. We aimed to investigate peak levels and dynamics IgG avidity maturation over time, correlate this clinical parameters, cytokines, T-cell responses.We did a longitudinal study patients who had recovered from COVID-19 up day 180 post-symptom onset by monitoring changes in using previously...
Chikungunya virus (CHIKV) infection induces arthralgia. The involvement of inflammatory cytokines and chemokines has been suggested, but very little is known about their secretion profile in CHIKV-infected patients.A case-control longitudinal study was performed that involved 30 adult patients with laboratory-confirmed fever. Their profiles clinical disease, viral load, immune mediators were investigated.When segregated into high load low groups during the acute phase, those viremia had...
Background.Plasmodium falciparum and Plasmodium vivax are responsible for most of the global burden malaria. Although accentuated pathogenicity P. occurs because sequestration mature erythrocytic forms in microvasculature, this phenomenon has not yet been noted vivax. The increasing number severe manifestations infections, similar to those observed malaria, suggests that key pathogenic mechanisms (eg, cytoadherence) might be shared by 2 parasites. Methods. Mature vivax–infected erythrocytes...
Abstract Chikungunya virus (CHIKV) is an alphavirus that causes chronic and incapacitating arthralgia in humans. To date, interactions between the immune system different stages of life cycle remain poorly defined. We demonstrated for first time CHIKV Ags could be detected vivo monocytes acutely infected patients. Using vitro experimental systems, whole blood purified monocytes, we confirmed growth sustained. with other leukocytes, induced a robust rapid innate response production specific...
Abstract Chikungunya virus (CHIKV) is an alphavirus that causes chronic and incapacitating arthralgia in humans. Injury to the joint believed occur because of viral host immune-mediated effects. However, exact involvement different immune mediators CHIKV-induced pathogenesis unknown. In this study, we assessed roles T cells primary CHIKV infection, replication dissemination, persistence, as well mediation disease severity adult RAG2−/−, CD4−/−, CD8−/−, wild-type C57BL/6J mice depleted CD4+...
Blood neutrophil homeostasis is essential for successful host defense against invading pathogens. Circulating counts are positively regulated by CXCR2 signaling and negatively the CXCR4–CXCL12 axis. In particular, G-CSF, a known signaler, plerixafor, CXCR4 antagonist, have both been shown to correct neutropenia in human patients. G-CSF directly induces mobilization from bone marrow (BM) into blood, but mechanisms underlying plerixafor-induced neutrophilia remain poorly defined. Using...
ABSTRACT The epidemiology of malaria in “low-transmission” areas has been underestimated. Molecular detection methods have revealed higher prevalences than conventional microscopy or rapid diagnostic tests, but these typically evaluate finger-prick capillary blood samples (∼5 μl) and therefore cannot detect parasite densities <200/ml. Their use underestimates true carriage rates. To characterize the low-transmission settings plan elimination strategies, more sensitive quantitative PCR...