A5020916833- Immunotherapy and Immune Responses
- Immune cells in cancer
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Malaria Research and Control
- Neurogenesis and neuroplasticity mechanisms
- Reproductive System and Pregnancy
- Mosquito-borne diseases and control
- vaccines and immunoinformatics approaches
- Dermatology and Skin Diseases
- Phagocytosis and Immune Regulation
- HIV Research and Treatment
- Chemokine receptors and signaling
- Science Education and Perceptions
- Cardiomyopathy and Myosin Studies
- Angiogenesis and VEGF in Cancer
- Cardiovascular Health and Disease Prevention
- Muscle Physiology and Disorders
- Social Media in Health Education
- Immune responses and vaccinations
- Extracellular vesicles in disease
Agency for Science, Technology and Research
2014-2023
Singapore Immunology Network
2013-2023
Institute of Molecular and Cell Biology
2022-2023
Microglia are the resident macrophages of central nervous system and associated with pathogenesis many neurodegenerative brain inflammatory diseases; however, origin adult microglia remains controversial. We show that postnatal hematopoietic progenitors do not significantly contribute to homeostasis in brain. In contrast macrophage populations, we develop mice lack colony stimulating factor-1 (CSF-1) but absent CSF-1 receptor-deficient mice. vivo lineage tracing studies established derive...
Tissue macrophages have a split personality Resident tissue (RTMs) reside in various tissue-specific niches during development. They evince microenvironment-directed phenotypes that support host defense and homeostasis. Chakarov et al. used single-cell RNA sequencing fate-mapping of murine lung RTMs to interrogate RTM-subset heterogeneity, interrelationships, ontogeny (see the Perspective by Mildner Yona). In addition alveolar macrophages, they identified two different interstitial...
Mouse and human dendritic cells (DCs) are composed of functionally specialized subsets, but precise interspecies correlation is currently incomplete. Here, we showed that murine lung gut lamina propria CD11b+ DC populations were comprised two subsets: FLT3- IRF4-dependent CD24+CD64− DCs contaminating CSF-1R-dependent CD24−CD64+ macrophages. Functionally, loss CD24+CD11b+ abrogated CD4+ T cell-mediated interleukin-17 (IL-17) production in steady state after Aspergillus fumigatus challenge....
Langerhans cells (LCs) are the dendritic (DCs) of epidermis, forming one first hematopoietic lines defense against skin pathogens. In contrast to other DCs, LCs arise from precursors that seed before birth. However, origin these embryonic remains unclear. Using in vivo lineage tracing, we identify a wave yolk sac (YS)-derived primitive myeloid progenitors onset fetal liver hematopoiesis. YS migrate embryo proper, including prospective skin, where they give rise LC precursors, and brain...
Dendritic cell (DC)-mediated cross-presentation of exogenous antigens acquired in the periphery is critical for initiation CD8(+) T responses. Several DC subsets are described human tissues but migratory cross-presenting DCs have not been isolated, despite their potential importance immunity to pathogens, vaccines, and tumors tolerance self. Here, we identified a CD141(hi) present interstitial dermis, liver, lung that was distinct from majority CD1c(+) CD14(+) tissue superior at soluble...
Dendritic cells (DC) are professional antigen-presenting that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) cytometry by time-of-flight (CyTOF)-to identify blood CD123+CD33+CD45RA+ precursors (pre-DC). Pre-DC share surface markers with plasmacytoid (pDC) but have...
Patients with pancreatic ductal adenocarcinoma (PDAC) face a clinically intractable disease poor survival rates, attributed to exceptionally high levels of metastasis. Epithelial-to-mesenchymal transition (EMT) is pronounced at inflammatory foci within the tumor; however, immunological mechanisms promoting tumor dissemination remain unclear. It well established that tumors exhibit Warburg effect, preferential use glycolysis for energy production, even in presence oxygen, support rapid...
Microglia, the brain resident macrophages, critically shape forebrain neuronal circuits. However, their precise function in cerebellum is unknown. Here we show that human and mouse cerebellar microglia express a unique molecular program distinct from microglia. Cerebellar microglial identity was driven by CSF-1R ligand CSF-1, independently of alternate ligand, IL-34. Accordingly, CSF-1 depletion Nestin+ cells led to severe transcriptional alterations microglia, while remained intact....
Background Infection with Plasmodium berghei ANKA (PbA) in susceptible mice induces a syndrome called experimental cerebral malaria (ECM) severe pathologies occurring various mouse organs. Immune mediators such as T cells or cytokines have been implicated the pathogenesis of ECM. Red blood infected PbA parasites shown to accumulate brain and other tissues during infection. This accumulation is thought be involved PbA–induced pathologies, which mechanisms are poorly understood. Methods...
It is well established that Ly6Chi monocytes develop from common monocyte progenitors (cMoPs) and reside in the bone marrow (BM) until they are mobilized into circulation. In our study, we found BM not a homogenous population, as current data would suggest. Using computational analysis approaches to interpret multidimensional datasets, demonstrate consist of two distinct subpopulations (CXCR4hi CXCR4lo subpopulations) both mice humans. Transcriptome studies vivo assays revealed functional...
Zika virus (ZIKV) infections have been linked with neurological complications and congenital syndrome. Given the high level of homology between ZIKV related flavivirus dengue (DENV), we investigated cross-reactivity using a panel DENV human mAbs. A majority mAbs showed binding to virions, several exhibiting neutralizing capacities against in vitro. Three best ZIKV-neutralizing were found recognize diverse epitopes on envelope (E) glycoprotein: highly conserved fusion-loop peptide,...
The diversity of mononuclear phagocyte (MNP) subpopulations across tissues is one the key physiological characteristics immune system. Here, we focus on understanding metabolic variability MNPs through network analysis applied to three large-scale transcriptional datasets: introduce (1) an ImmGen MNP open-source dataset 337 samples 26 tissues; (2) a myeloid subset Phase I (202 samples); and (3) mouse single-cell RNA sequencing (scRNA-seq) (51,364 cells) assembled based Tabula Muris Senis. To...
The human dendritic cell (DC) lineage has recently been unraveled by high-dimensional mapping, revealing the existence of a discrete new population blood circulating DC precursors (pre-DCs). Whether this possesses specific functional features as compared to other subset upon pathogen encounter remained be evaluated. A unique feature pre-DCs among DCs is their constitutive expression viral adhesion receptor Siglec-1. Here, we show that pre-DCs, but not subsets, are susceptible infection HIV-1...
The dendritic cells (DCs) present in lymphoid and non-lymphoid organs are generated from progenitors with myeloid-restricted potential. However, the thymus a major subset of DCs expressing CD8α langerin (CD207) appears to stand apart all other that it is thought derive Using mice fluorescent reporter diphtheria toxin receptor under control cd207 gene, we demonstrated CD207(+) CD8α(+) thymic do not share common origin T but originate intrathymic precursors express markers normally on...