A5014898667- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Phagocytosis and Immune Regulation
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Cell Image Analysis Techniques
- Cancer Mechanisms and Therapy
- Microfluidic and Bio-sensing Technologies
- Cancer Research and Treatments
- Cellular Mechanics and Interactions
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Endometrial and Cervical Cancer Treatments
- Ferroptosis and cancer prognosis
- Hippo pathway signaling and YAP/TAZ
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Renal cell carcinoma treatment
National Taiwan University
2004-2025
National University of Singapore
2016-2025
National University Cancer Institute, Singapore
2014-2024
Min Sheng General Hospital
2024
Linkou Chang Gung Memorial Hospital
2023
National University Health System
2013-2021
National University Hospital
2009-2020
Clinical Nutrition Research Centre
2016
Zero to Three
2015
University Health System
2015
The phenotypic transformation of well-differentiated epithelial carcinoma into a mesenchymal-like state provides cancer cells with the ability to disseminate locally and metastasise. Different degrees epithelial–mesenchymal transition (EMT) have been found occur in carcinomas from breast, colon ovarian (OC), among others. Numerous studies focused on bona fide mesenchymal states but rarely intermediate states. In this study, we describe model system for appraising spectrum EMT using 43...
Patients with pancreatic ductal adenocarcinoma (PDAC) face a clinically intractable disease poor survival rates, attributed to exceptionally high levels of metastasis. Epithelial-to-mesenchymal transition (EMT) is pronounced at inflammatory foci within the tumor; however, immunological mechanisms promoting tumor dissemination remain unclear. It well established that tumors exhibit Warburg effect, preferential use glycolysis for energy production, even in presence oxygen, support rapid...
Epithelial-mesenchymal transition (EMT) plays a critical role in the early stages of dissemination carcinoma leading to metastatic tumors, which are responsible for over 90% all cancer-related deaths. Current therapeutic regimens, however, have been ineffective cure cancer, thus an urgent need exists revisit existing protocols and improve efficacy newly developed therapeutics. Strategies based on preventing EMT could potentially contribute improving outcome advanced stage cancers. To achieve...
Overexpression of chemokine receptor type 4 (CXCR4) has been found to be associated with increased cell proliferation, metastasis and also act as an indicator poor prognosis in patients breast cancer. Therefore, new agents that can abrogate CXCR4 expression have potential against cancer metastasis. In this study, we examined the effect thymoquinone (TQ), derived from seeds Nigella sativa, on regulation cells. TQ was inhibit MDA-MB-231 triple negative (TNBC) cells a dose- time-dependent...
Abstract Epithelial-mesenchymal transition (EMT), a biological process by which polarized epithelial cells convert into mesenchymal phenotype, has been implicated to contribute the molecular heterogeneity of ovarian cancer (EOC). Here we report that transcription factor—Grainyhead-like 2 (GRHL2) maintains phenotype. EOC tumours with lower GRHL2 levels are associated Mes/Mesenchymal subtype and poorer overall survival. shRNA-mediated knockdown in an phenotype results EMT changes, increased...
Ovarian cancer (OC) can be classified into five biologically distinct molecular subgroups: epithelial-A (Epi-A), Epi-B, mesenchymal (Mes), Stem-A and Stem-B. Among them, expresses genes relating to stemness is correlated with poor clinical prognosis. In this study, we show that frizzled family receptor 7 (FZD7), a for Wnt signalling, overexpressed in the subgroup. To elucidate functional roles of FZD7, used an RNA interference gene knockdown approach three cell lines: CH1, PA1 OV-17R....
AXL inhibitors may prolong survival in a subset of patients with advanced ovarian cancer.
Although cancer-associated fibroblasts (CAFs) are viewed as a promising therapeutic target, the design of rational therapy has been hampered by two key obstacles. First, attempts to ablate CAFs have resulted in significant toxicity because currently used biomarkers cannot effectively distinguish activated from non-cancer associated and mesenchymal progenitor cells. Second, it is unclear whether different organs molecular functional properties that necessitate organ-specific designs. Our...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus infection in pregnancy is associated with higher incidence of placental dysfunction, referred to by a few studies as 'preeclampsia-like syndrome'. However, the mechanisms underpinning SARS-CoV-2-induced malfunction are still unclear. Here, we investigated whether transcriptional architecture placenta altered response SARS-CoV-2 infection.
Microwell technology has revolutionized many aspects of in vitro cellular studies from 2D traditional cultures to 3D vivo‐like functional assays. However, existing lithography‐based approaches are often costly and time‐consuming. This study presents a rapid, low‐cost prototyping method CO 2 laser ablation conventional untreated culture dish create concave microwells used for generating multicellular aggregates, which can be readily available general laboratories. Polymethylmethacrylate...